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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/52101
Title: Dissemination of carbapenem-resistance among multidrug resistant Pseudomonas aeruginosa carrying metallo-beta-lactamase genes, including the novel bla<inf>IMP-65</inf> gene in Thailand
Authors: Piyatip Khuntayaporn
Wariya Yamprayoonswat
Montri Yasawong
Mullika Traidej Chomnawang
Mahidol University
Chulabhorn Royal Academy
Keywords: Medicine
Issue Date: 1-Jan-2019
Citation: Infection and Chemotherapy. Vol.51, No.2 (2019), 107-118
Abstract: Copyright © 2019 by The Korean Society of Infectious Diseases and Korean Society for Antimicrobial Therapy. Background: Pseudomonas aeruginosa is considered as one of the most emerging threats in this century. Serious infections caused by this pathogen are often treated by carbapenems which are the last resource of antibiotics. Metallo-beta-lactamases (MBLs) production is one of the most important carbepenem resistance mechanisms and is usually related with nosocomial infections caused by P. aeruginosa. This study was aimed to determine the prevalence of MBL genes and distribution pattern of MBLs producing P. aeruginosa strains in Thailand. Materials and Methods: Specific primers were designed to detect MBL genes including IMP-, VIM-, and NDM-type MBL genes. Multilocus sequence typing method was used to determine the dissemination pattern of carbapenem-resistance among multidrug-resistant (CR-MDR) P. aeruginosa. Results: A total of 153 P. aeruginosa clinical isolates were characterized as CR-MDR. Among those, 31 P. aeruginosa clinical isolates (20.3%) presented metallo-beta-lactamase genes which could be divided into VIM-type (14 strains) and IMP-type (17 strains). blaIMP-1, blaIMP-13, blaIMP-14a, and blaVIM-2 genes were detected. Moreover, a novel IMP-type MBL, blaIMP-65 was discovered and it was demonstrated to be the unique group of MBLs in Thailand. It was of interest that ST235 was the major ST type in Thailand followed by ST964 and ST111 and ST235 was detected in both MBL harboring and non-MBL harboring strains. Conclusion: This study reported the dissemination of MBL gene including novel MBL, blaIMP-65. This study was also demonstrated major ST of P. aeruginosa which was ST235, followed by ST964 and ST111. Moreover, it is also the first report on many P. aeruginosa STs in Thailand: ST273, ST292, ST621, ST1584, and ST1816 which emphasized the dissemination trait difference of MBLs harboring P. aeruginosa in Thailand.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/52101
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85068852861&origin=inward
ISSN: 20926448
20932340
Appears in Collections:Scopus 2019

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