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Title: Identification of apigenin and luteolin in Artemisia annua l. for the quality control
Authors: Rattana Phadungrakwittaya
Sirikul Chotewuttakorn
Manoon Piwtong
Onusa Thamsermsang
Tawee Laohapand
Pravit Akarasereenont
Faculty of Medicine, Siriraj Hospital, Mahidol University
Keywords: Medicine
Issue Date: 1-Jan-2019
Citation: Siriraj Medical Journal. Vol.71, No.3 (2019), 240-245
Abstract: © 2019, Siriraj Medical Journal. Objective: To identify active compounds and establish the chemical fingerprint of Artemisia annua L. for the quality control. Methods: Thin-layer chromatography (TLC) conditions were developed to screen for 2 common flavonoids (apigenin and luteolin). Three mobile phases were used to isolate these flavonoids in 80% ethanolic extract of A. annua. Hexane: ethyl acetate: acetic acid (31:14:5, v/v) and toluene: 1,4-dioxane: acetic acid (90:25:4, v/v) were used in normal phase TLC (NP-TLC), and 5.5% formic acid in water: methanol (50:50, v/v) were used in reverse phase TLC (RP-TLC). Chromatograms were visualized under visible light after spraying with Fast Blue B Salt. Apigenin and luteolin bands were checked by comparing their Rf values and UV-Vis absorption spectra with reference markers. Results: Apigenin and luteolin were simultaneously detected with good specificity in RP-TLC condition, while only apigenin was detected in NP-TLC condition. Apigenin band intensity was higher than luteolin band intensity in both conditions. Conclusion: This knowledge can be applied to the development of quality control assessments to ensure product efficacy and consistency.
ISSN: 22288082
Appears in Collections:Scopus 2019

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