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dc.contributor.authorJirawan Panachanen_US
dc.contributor.authorDaranee Chokchaichamnankiten_US
dc.contributor.authorChurat Weeraphanen_US
dc.contributor.authorChantragan Srisomsapen_US
dc.contributor.authorPatarabutr Masaratanaen_US
dc.contributor.authorSuneerat Hatairakthamen_US
dc.contributor.authorNarumol Panichkulen_US
dc.contributor.authorJisnuson Svastien_US
dc.contributor.authorRuchaneekorn W. Kalpravidhen_US
dc.contributor.otherChulabhorn Research Instituteen_US
dc.contributor.otherFaculty of Medicine, Siriraj Hospital, Mahidol Universityen_US
dc.contributor.otherPrince of Songkla Universityen_US
dc.identifier.citationHematology (United Kingdom). Vol.24, No.1 (2019), 300-307en_US
dc.description.abstract© 2019, © 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. Objective: Iron overload and oxidative stress are the major causes of serious complications and mortality in thalassemic patients. Our previous work supports the synergistic effects of antioxidant cocktails (curcuminoids or vitamin E, N-acetylcysteine, and deferiprone) in treatment of β-thalassemia/Hb E patients. This further 2-DE-based proteomic study aimed to identify the plasma proteins that expressed differentially in response to antioxidant cocktails. Methods: Frozen plasma samples of ten normal subjects and ten β-thalassemia/Hb E patients at three-time points (baseline, month 6, and month 12) were reduced the dynamic range of proteome using ProteoMiner kit and separated proteins by two-dimensional gel electrophoresis. Differentially expressed proteins were identified using tandem mass spectrometry. Several plasma proteins were validated by ELISA and Western blot analysis. Results: Thirteen and 11 proteins were identified with altered expression levels in the curcuminoids- and vitamin E cocktail groups, respectively. The associations between vitronectin (VTN) expression and total bilirubin levels, as well as between serum paraoxonase/arylesterase 1 (PON1) expression and blood reactive oxygen species were observed. Validation results were consistent with proteomics results. Discussion and conclusions: These plasma proteins may provide better understanding of the mechanisms underlying the therapeutic effects of antioxidant cocktails in thalassemic patients.en_US
dc.rightsMahidol Universityen_US
dc.titleDifferentially expressed plasma proteins of β-thalassemia/hemoglobin E patients in response to curcuminoids/vitamin E antioxidant cocktailsen_US
Appears in Collections:Scopus 2019

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