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Title: Detection and identification of Escherichia coli and Staphylococcus aureus by Raman spectroscopy
Authors: Sirichayaporn Huayhongthong
Piyatip Khuntayaporn
Pagakrong Wanapaisan
Krit Thirapanmethee
Chutima Phechkrajang
Nattawut Chareonthai
Mullika Traidej Chomnawang
ปิยทิพย์ ขันตยาภรณ์
ผกากรอง วนไพศาล
กฤษณ์ ถิรพันธุ์เมธี
ชุติมา เพชรกระจ่าง
ณัฐวุฒิ เจริญไทย
มัลลิกา (ไตรเดช) ชมนาวัง
Mahidol University. Faculty of Pharmacy. Department of Microbiology
Mahidol University. Faculty of Pharmacy. Department of Pharmaceutical Chemistry
Mahidol University. Faculty of Pharmacy. Department of Manufacturing Pharmacy
Keywords: Raman spectroscopy;Pathogen;Detection;Principal component analysis;Food
Issue Date: 2019
Abstract: Food-borne infection is one of the main reasons affecting public health problem worldwide. Therefore, food safety is highly concerned for the food production system and the rapid-testing process is still required in order to prevent contamination before commercially distributed to consumers. Bacterial detection process is becoming one of the most critical steps in the food production chain. Raman spectroscopy is considered as an alternative technique to rapidly detect and identify the microorganism. In this study, Raman spectroscopy was applied to detect and identify the food-borne pathogens, including Gram-positive and Gram-negative bacteria. The multivariate statistical analysis of principal component analysis (PCA) was used to distinguish these microorganisms based on their Raman spectroscopic fingerprints. Our study demonstrated that the physiological state of microorganism species had an influence on the identification sensitivity. In addition, there were significant differences between Raman spectra of Gram-negative and Gram-positive bacteria. The resultant plots showed that the bacterial spectra displayed degree of discrimination and accuracy identification in calculating accounted for 73.39% of total variance of the first and second principal components (PC-1 and PC-2) of the analyzed data sets. In conclusion, the study suggested that Raman spectroscopy could be utilized for food quality control with reliable and rapid identification.
Description: The 1st Pharmaceutical Sciences Asia Conference 2019 Theme : Pharmaceutical Sciences toward Health Innovation in the Disruptive Era. Bangkok Midtown Hotel, Thailand. August 22, 2019, page 45-48
Appears in Collections:PY-Proceeding Document

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