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Title: Exosome aggregation mediated stop-flow paper-based portable device for rapid exosome quantification
Authors: Boonta Chutvirasakul
Nantana Nuchtavorn
Leena Suntornsuk
Yong Zeng
University of Kansas, Lawrence
Mahidol University
University of Kansas Cancer Center
Srinakharinwirot University
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry
Issue Date: 1-Mar-2020
Citation: Electrophoresis. Vol.41, No.5-6 (2020), 311-318
Abstract: © 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim Exosome quantification is important for estimation of informative messengers (e.g., proteins, lipids, RNA, etc.) involving physiological and pathological effects. This work aimed to develop a simple and rapid distance-based paper portable device using exosome-capture vesicles (polydiacetylene conjugated with antiCD81) for exosome quantification in cell cultures. This novel concept relied on distinct aggregation of exosomes and exosome-capture vesicles leading to different solvent migration. Distances of the migration were used as signal readouts, which could be detected by naked eye. PDA-antiCD81 as exosome-capture vesicles were optimized (e.g., size, reaction ratio, and concentration) and the paper designs were investigated (e.g., diameter of sample reservoir and lamination layer) to enhance the solvent stop-flow effects. Finally, exosome screening on three cell culture samples (COLO1, MDA-MB-231, and HuR-KO1 subclone) was demonstrated. The method could linearly measure exosome concentrations in correlation with solvent migration distances in the range of 106–1010 particles/mL (R2 > 0.98) from the cell culture samples. The exosome concentration measurements by the developed device were independently assessed by nanoparticle tracking analysis. Results demonstrated no statistically significant difference (p > 0.05) by t-test. This low-cost and rapid device allows a portable platform for exosome quantification without the requirement of expensive equipment and expertise of operation. The developed device could potentially be useful for quantification of other biomarker-related extracellular vesicles.
ISSN: 15222683
Appears in Collections:Scopus 2020

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