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Title: Sensitive detection of albuminuria by graphene oxide-mediated fluorescence quenching aptasensor
Authors: Wireeya Chawjiraphan
Chayachon Apiwat
Khoonsake Segkhoonthod
Kiatnida Treerattrakoon
Preedee Pinpradup
Nuankanya Sathirapongsasuti
Prapasiri Pongprayoon
Patraporn Luksirikul
Patcharee Isarankura-Na-Ayudhya
Deanpen Japrung
Kasetsart University
Thailand National Nanotechnology Center
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Thammasat University
Keywords: Chemistry;Physics and Astronomy
Issue Date: 15-Apr-2020
Citation: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. Vol.231, (2020)
Abstract: © 2020 Elsevier B.V. Albuminuria is a pathological condition wherein the human serum albumin (HSA) protein is present in abnormally excess amounts in the urine. A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albumin in urine samples and HSA in serum samples. The aptamer-bound HSA used in this aptasensor has hairpin structures, which are characteristic of the aptamer binding site. The limit of detection of the developed platform is 0.05 μg·mL−1 and the detection range is 0.1–14.0 μg·mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with kidney diseases. This approach can be modified to measure HSA using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for other standard automated methods. Therefore, this aptasensor has significant potential for commercialization and wide-scale public use.
ISSN: 13861425
Appears in Collections:Scopus 2020

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