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Title: Venomics and cellular toxicity of Thai pit vipers (Trimeresurus macrops and T. hageni)
Authors: Supeecha Kumkate
Lawan Chanhome
Tipparat Thiangtrongjit
Jureeporn Noiphrom
Panithi Laoungboa
Orawan Khow
Taksa Vasaruchapong
Siravit Sitprija
Narongsak Chaiyabutr
Onrapak Reamtong
Thai Red Cross Agency
Mahidol University
Queen Saovabha Memorial Institute
Keywords: Environmental Science;Pharmacology, Toxicology and Pharmaceutics
Issue Date: 1-Jan-2020
Citation: Toxins. Vol.12, No.1 (2020)
Abstract: © 2020 by the authors. The two venomous pit vipers, Trimeresurus macrops and T. hageni, are distributed throughout Thailand, although their abundance varies among different areas. No species-specific antivenom is available for their bite victims, and the only recorded treatment method is a horse antivenom raised against T. albolabris crude venom. To facilitate assessment of the cross-reactivity of heterologous antivenoms, protein profiles of T. macrops and T. hageni venoms were explored using mass-spectrometry-based proteomics. The results show that 185 and 216 proteins were identified from T. macrops and T. hageni venoms, respectively. Two major protein components in T. macrops and T. hageni venoms were snake venom serine protease and metalloproteinase. The toxicity of the venoms on human monocytes and skin fibroblasts was analyzed, and both showed a greater cytotoxic effect on fibroblasts than monocytic cells, with toxicity occurring in a dose-dependent rather than a time-dependent manner. Exploring the protein composition of snake venom leads to a better understanding of the envenoming of prey. Moreover, knowledge of pit viper venomics facilitates the selection of the optimum heterologous antivenoms for treating bite victims.
ISSN: 20726651
Appears in Collections:Scopus 2020

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