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Title: Novel salivary gland allergens from tropical mosquito species and IgE reactivity in allergic patients
Authors: Anunya Opasawatchai
Watchareewan Yolwong
Walairat Thuncharoen
Nanthicha Inrueangsri
Sulak Itsaradisaikul
Cherapat Sasisakulporn
Wanlapa Jotikasthira
Oranart Matangkasombut
Onrapak Reamtong
Wiparat Manuyakorn
Wisuwat Songnuan
Ponpan Matangkasombut
Chulalongkorn University
Chulabhorn Research Institute
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Feb-2020
Citation: World Allergy Organization Journal. Vol.13, No.2 (2020)
Abstract: © 2020 The Authors Background: Mosquito allergy is common in tropical countries but remains under-diagnosed. This may be due to the lack of knowledge and diagnostic tools for tropical mosquito allergens. Objective: We aimed to characterize allergens from tropical mosquito species and investigate IgE reactivity in mosquito-allergic patients to the salivary gland proteins from these mosquitoes. Methods: Salivary gland extract (SGE) from 4 mosquito species, highly distributed in the tropics, including Aedes aegypti, Aedes albopictus, Culex quinquefasciatus, and Anopheles dirus b, were studied. SGE-specific IgE and IgG ELISA were developed, and serum from 64 mosquito-allergic and 22 non-allergic healthy control subjects was assayed. Further investigations using IgE-immunoblots followed by mass spectrometry analysis were performed to identify and characterize allergens from each species. Results: Mosquito-allergic subjects have detectable serum IgE to SGE derived from local mosquito species, while the IgE levels to Aedes communis using commercially available ELISA were mostly minimal. IgE-immunoblot analysis and mass spectrometry identified 5 novel mosquito allergens from A. albopictus (Aed al 2, Aed al 3), C. quinquefasciatus (Cul q 2.01, Cul q 3), and A. dirus b (Ano d 2). Interestingly, 4 of the 5 new allergens belong to the D7 protein family. Conclusions & clinical relevance: Five novel allergens from 3 tropical mosquito species were characterized. The majority of mosquito-allergic subjects who live in the tropics have IgE reactivity to these allergens. Our study paves the way for the development of diagnostic tests, component-resolved diagnostics, and future immunotherapy for mosquito allergy in tropical countries.
ISSN: 19394551
Appears in Collections:Scopus 2020

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