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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/53928
Title: In situ identification of polyhydroxyalkanoate (PHA)-accumulating microorganisms in mixed microbial cultures under feast/famine conditions
Authors: Donhatai Sruamsiri
Parinda Thayanukul
Benjaporn Boonchayaanant Suwannasilp
Chulalongkorn University
Mahidol University
King Mongkut s University of Technology Thonburi
Research Network of NANOTEC-CU (RNN)
Keywords: Multidisciplinary
Issue Date: 1-Dec-2020
Citation: Scientific Reports. Vol.10, No.1 (2020)
Abstract: © 2020, The Author(s). The accumulation of plastic waste in the environment has become a serious environmental problem worldwide. Biodegradable plastics, such as polyhydroxyalkanoate (PHA), could serve as green alternatives to petroleum-based plastics. In this study, a mixed microbial culture was enriched under feast/famine conditions using a sequencing batch reactor (SBR) with acetate as a carbon source. The enrichment could accumulate a maximum PHA concentration of 32.3% gPHA/g mixed liquor suspended solids (MLSS) in the 12th cycle of SBR operation. The microbial community in this sludge sample was analyzed using 16 S rRNA gene amplicon sequencing (MiSeq). The results showed the dominance of Proteobacteria, represented by Alphaproteobacteria (13.26% of total sequences), Betaproteobacteria (51.37% of total sequences), and Gammaproteobacteria (23.44% of total sequences). Thauera (Betaproteobacteria) had the highest relative abundance, accounting for 48.88% of the total sequences. PHA-accumulating microorganisms in the enrichment were detected using fluorescence in situ hybridization (FISH) and a fluorescent dye, Nile blue A. Alphaproteobacteria and Betaproteobacteria were capable of accumulating PHA, while no Gammaproteobacteria were detected. Thauera spp. from Betaproteobacteria constituted 80.3% of the total PHA accumulating cells.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/53928
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85081003030&origin=inward
ISSN: 20452322
Appears in Collections:Scopus 2020

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