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dc.contributor.authorParinyachat Somchaien_US
dc.contributor.authorKriengkrai Phongkitkarunen_US
dc.contributor.authorPatipark Kueanjindaen_US
dc.contributor.authorSupawan Jamnongsongen_US
dc.contributor.authorKulthida Vaeteewoottacharnen_US
dc.contributor.authorVor Luviraen_US
dc.contributor.authorSeiji Okadaen_US
dc.contributor.authorSiwanon Jirawatnotaien_US
dc.contributor.authorSomponnat Sampattavanichen_US
dc.contributor.otherFaculty of Medicine, Khon Kaen Universityen_US
dc.contributor.otherKumamoto Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherFaculty of Medicine, Siriraj Hospital, Mahidol Universityen_US
dc.identifier.citationScientific Reports. Vol.10, No.1 (2020)en_US
dc.description.abstract© 2020, The Author(s). Wound healing assay is a simple and cost-effective in vitro assay for assessing therapeutic impacts on cell migration. Its key limitation is the possible confoundment by other cellular phenotypes, causing misinterpretation of the experimental outcome. In this study, we attempted to address this problem by developing a simple analytical approach for scoring therapeutic influences on both cell migration and cell death, while normalizing the influence of cell growth using Mitomycin C pre-treatment. By carefully mapping the relationship between cell death and wound closure rate, contribution of cell death and cell migration on the observed wound closure delay can be quantitatively separated at all drug dosing. We showed that both intrinsic cell motility difference and extrinsic factors such as cell seeding density can significantly affect final interpretation of therapeutic impacts on cellular phenotypes. Such discrepancy can be rectified by using the actual wound closure time of each treatment condition for the calculation of phenotypic scores. Finally, we demonstrated a screen for strong pharmaceutical inhibitors of cell migration in cholangiocarcinoma cell lines. Our approach enables accurate scoring of both migrastatic and cytotoxic effects, and can be easily implemented for high-throughput drug screening.en_US
dc.rightsMahidol Universityen_US
dc.titleNovel Analytical Platform For Robust Identification of Cell Migration Inhibitorsen_US
Appears in Collections:Scopus 2020

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