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|Title:||A rapid and simple method for routine determination of antibiotic sensitivity to biofilm populations of Pseudomonas aeruginosa|
|Authors:||Dhammika Leshan Wannigama|
Robin James Storer
University of Western Australia
QIMR Berghofer Medical Research Institute
Mae Fah Luang University
The Westmead Institute for Medical Research
|Citation:||Annals of Clinical Microbiology and Antimicrobials. Vol.19, No.1 (2020)|
|Abstract:||© 2020 The Author(s). Treatment of infections by Pseudomonas aeruginosa forming biofilms after antimicrobial testing on planktonic bacteria can result in substantial failure. Therefore, we offer a robust and simple experimental platform to test the impact of antimicrobials on biofilms. Antibiotic response patterns varied uniquely within biofilm formation capacity and minimal biofilm eradication concentrations (MBECs) has a significantly better discriminatory power than minimum inhibitory concentrations (MICs) to differentiate the overall efficiency of antibiotics to eradicate biofilm. Our resazurin-based 96-well-plate platform is able to emulate bacterial responses to antibiotics under biofilm conditions in a fast, simple, and cost-effective screening method adaptable to automation, and warrants trials in the clinic.|
|Appears in Collections:||Scopus 2020|
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