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dc.contributor.authorArinta Agnie Dewantarien_US
dc.contributor.authorNattha Yongwattanaen_US
dc.contributor.authorPanwajee Payongsrien_US
dc.contributor.authorSawinee Seemakhanen_US
dc.contributor.authorSuparerk Borwornpinyoen_US
dc.contributor.authorAkio Ojidaen_US
dc.contributor.authorJirarut Wongkongkatepen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherKyushu Universityen_US
dc.identifier.citationMolecules. Vol.25, No.9 (2020)en_US
dc.description.abstract© 2020 by the authors. A rapid, sensitive and reliable indicator displacement assay (IDA) for specific detection of 2'- and 3'-deoxyadenosine (2'-dAde and 3'-dAde), the latter is also known as cordycepin, was established. The formation of inclusion complex between protonated acridine orange (AOH+) and cucurbit[7]uril (CB7) resulted in the hypochromic shift of fluorescent emission from 530 nm to 512 nm. Addition of cordycepin to the highly fluorescent AOH+/CB7 complex resulted in a unique tripartite AOH+/CB7/dAde complex with diminished fluorescence, and such reduction in emission intensity serves as the basis for our novel sensing system. The detection limits were 11 and 82 µM for 2'- and 3'-deoxyadenosine, respectively. The proposed method also demonstrated high selectivity toward 2'- and 3'-deoxyadenosine, owing to the inability of other deoxynucleosides, nucleosides and nucleotides commonly found in Cordyceps spp. to displace the AOH+ from the AOH+/CB7 complex, which was confirmed by isothermal titration calorimetry (ITC), UV-Visible and proton nuclear magnetic resonance (1H-NMR) spectroscopy. Our method was successfully implemented in the analysis of cordycepin in commercially available Ophiocordyceps and Cordyceps supplements, providing a novel and effective tool for quality assessment of these precious fungi with several health benefits.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectPharmacology, Toxicology and Pharmaceuticsen_US
dc.titleFluorescence detection of deoxyadenosine in Cordyceps spp. By indicator displacement assayen_US
Appears in Collections:Scopus 2020

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