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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/56218
Title: Development of Nested PCR-Heteroduplex Mobility Assay for Determination of Genetic Diversity in the Block 2 Region of the Plasmodium falciparum Merozoite Surface Protein 1 Gene
Authors: Kanyanan Kritsiriwuthinan
Warunee Ngrenngarmlert
Sakone Sunantaraporn
Anna Jehmah
Chulalongkorn University
Rangsit University
Mahidol University
Maelan Hospital
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jan-2020
Citation: Journal of Parasitology Research. Vol.2020, (2020)
Abstract: © 2020 Kanyanan Kritsiriwuthinan et al. Genetic diversity of Plasmodium parasite has significantly related to malaria control and vaccine development. The P. falciparum merozoite surface protein 1 (Pfmsp1) gene is a commonly used molecular marker to differentiate genetic diversity. This study is aimed at developing a nested PCR-Heteroduplex Mobility Assay (nPCR-HMA) for determination of the block 2 of the Pfmsp1 gene. The MAD20 family allele of P. falciparum was used as a control for optimization of the annealing and polyacrylamide gel electrophoresis conditions. In order to evaluate the developed nPCR-HMA, 8 clinical P. falciparum isolates were examined for allelic variants. The results revealed 9 allelic variants. Our study indicated that the successful nPCR-HMA with good precision and accuracy offers a more rapid, efficient, and cheap method for large-scale molecular epidemiological studies as compared to nucleotide sequencing.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/56218
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85084078248&origin=inward
ISSN: 20900031
20900023
Appears in Collections:Scopus 2020

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