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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/57584
Title: New records and DNA barcoding of deer flies, Chrysops (Diptera: Tabanidae) in Thailand
Authors: Tanasak Changbunjong
Thekhawet Weluwanarak
Poonyapat Sedwisai
Jiraporn Ruangsittichai
Gerard Duvallet
Theeraphap Chareonviriyaphap
Kasetsart University
Mahidol University
Universite Paul-Valery Montpellier III
Keywords: Agricultural and Biological Sciences;Immunology and Microbiology;Medicine;Veterinary
Issue Date: 1-Oct-2020
Citation: Acta Tropica. Vol.210, (2020)
Abstract: © 2020 Elsevier B.V. Chrysops spp. or deer flies (Diptera: Tabanidae) are hematophagous flies of medical and veterinary importance and some species are important vectors of Trypanosoma evansi, the causative agent of surra in Thailand. However, data regarding deer fly species and their molecular identification are limited. Accurate species identification will indicate the appropriate control measures. In this study, an entomological survey of deer flies from different sites in Thailand between May 2018 and June 2019 were conducted. In addition, mitochondrial cytochrome oxidase subunit I (COI) barcoding region was used for species identification. A total of 82 females were collected and 6 species were identified. Of these, three species are new records for Thailand: C. designatus, C. fuscomarginalis and C. vanderwulpi bringing the species total found in Thailand to nine. The COI sequences revealed an intraspecific divergence of 0.0%–2.65% and an interspecific divergence of 7.03%–13.47%. Phylogenetic analysis showed that all deer fly species were clearly separated into distinct clusters according to morphologically identified species. These results indicated that COI barcodes were capable in discriminating between deer fly species on the basis of the barcoding gap and phylogenetic analysis. Therefore, DNA barcoding is a valuable tool for species identification of deer flies in Thailand.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/57584
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85086118811&origin=inward
ISSN: 18736254
0001706X
Appears in Collections:Scopus 2020

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