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Title: Effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs. In vitro cultures: A comparative analysis
Authors: Afifa Zaeem
Samantha Drouet
Sumaira Anjum
Razia Khurshid
Muhammad Younas
Jean Philippe Blondeau
Duangjai Tungmunnithum
Nathalie Giglioli‐guivarc’h
Christophe Hano
Bilal Haider Abbasi
Biomolécules et Biotechnologies Végétales (BBV)
CRMHT Centre de Recherches sur les Matériaux à Haute Température
Quaid-i-Azam University
Universite d'Orleans
Mahidol University
Virtual University of Pakistan
Kinnaird College for Women
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jun-2020
Citation: Biomolecules. Vol.10, No.6 (2020), 1-16
Abstract: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Linum usitatissimum biosynthesizes lignans and neolignans that are diet and medicinally valuable metabolites. In recent years, zinc oxide nanoparticles (ZnONPs) have emerged as potential elicitors for the enhanced biosynthesis of commercial secondary metabolites. Herein, we investigated the influence of biogenic ZnONPs on both seedlings and stem‐derived callus of L. usitatissimum. Seedlings of L. usitatissimum grown on Murashige and Skoog (MS) medium supplemented with ZnONPs (1–1000 mg/L) presented the highest antioxidant activity, total phenolic content, total flavonoid content, peroxidase and superoxide dismutase activities at 500 mg/L, while the maximum plantlet length was achieved with 10 mg/L. Likewise, the high-performance liquid chromatography (HPLC) analysis revealed the enhanced production of secoisolariciresinol diglucoside, lariciresinol diglucoside, dehydrodiconiferyl alcohol glucoside and guaiacylglycerol‐β‐coniferyl alcohol ether glucoside in the plantlets grown on the 500 mg/L ZnONPs. On the other hand, the stem explants were cultured on MS media comprising 1‐ naphthaleneacetic acid (1 mg/L) and ZnONPs (1–50 mg/L). The highest antioxidant and other activities with an enhanced rooting effect were noted in 25 mg/L ZnONP‐treated callus. Similarly, the maximum metabolites were also accumulated in 25 mg/L ZnONP‐treated callus. In both systems, the dose‐dependent production of reactive oxygen species (ROS) was recorded, resulting in oxidative damage with a more pronounced toxic effect on in vitro cultures. Altogether, the results from this study constitute a first comprehensive view of the impact of ZnONPs on the oxidative stress and antioxidant responses in seedlings vs. in vitro cultures.
ISSN: 2218273X
Appears in Collections:Scopus 2020

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