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dc.contributor.authorAttaphon Chamchoien_US
dc.contributor.authorSirada Srihirunen_US
dc.contributor.authorKittiphong Paiboonsukwongen_US
dc.contributor.authorThanaporn Sriwantanaen_US
dc.contributor.authorPeerawich Kongkaewen_US
dc.contributor.authorSuthat Fucharoenen_US
dc.contributor.authorKovit Pattanapanyasaten_US
dc.contributor.authorNathawut Sibmoohen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherFaculty of Medicine, Siriraj Hospital, Mahidol Universityen_US
dc.identifier.citationInternational Journal of Laboratory Hematology. (2020)en_US
dc.description.abstract© 2020 John Wiley & Sons Ltd Introduction: An increase in platelet activity is a contributing factor to vascular complications in hemoglobin E/β-thalassemia (HbE/β-thal). Plasma-free hemoglobin (Hb) increases in HbE/β-thal patients and correlates with platelet activation, but the levels of Hb-bound platelets have never been reported. In this study, we aimed to investigate the levels of Hb-bound platelets and its association with platelet activity in HbE/β-thal patients. Methods: Hb-bound platelets were measured by flow cytometry in 22 healthy subjects and 26 HbE/β-thal patients (16 nonsplenectomized and 10 splenectomized HbE/β-thal patients). Plasma Hb was measured by the chemiluminescence method based on the consumption of nitric oxide (NO) by Hb. Expression of P-selectin and activated glycoprotein (aGP) IIb/IIIa on platelets was measured by flow cytometry as a marker of platelet activity. Results: Both nonsplenectomized and splenectomized HbE/β-thal patients had higher levels of Hb-bound platelets and plasma Hb than healthy subjects. In vitro incubation of dialyzed Hb from patients with platelets of healthy subjects caused an increase in Hb-bound platelets, which was partially inhibited by anti-GPIbα antibody. Plasma Hb positively correlated with Hb-bound platelets. Platelet P-selectin expression at baseline and in response to adenosine diphosphate (ADP, 1 µM) stimulation was higher in nonsplenectomized and splenectomized HbE/β-thal patients than healthy subjects. The ADP-induced aGPIIb/IIIa expression on platelets was also higher in HbE/β-thal patients than healthy subjects. Hb-bound platelets correlated with baseline P-selectin expression and ADP-induced P-selectin expression. Conclusion: HbE/β-thal patients have increased Hb-bound platelets, which is associated with increased baseline platelet activation and reactivity.en_US
dc.rightsMahidol Universityen_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleHemoglobin-bound platelets correlate with the increased platelet activity in hemoglobin E/β-thalassemiaen_US
Appears in Collections:Scopus 2020

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