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Title: Whole-genome sequencing analysis of multidrug-resistant Mycobacterium tuberculosis from Java, Indonesia
Authors: Tryna Tania
Pratiwi Sudarmono
R. Lia Kusumawati
Andriansjah Rukmana
Wahyu Agung Pratama
Sanjib Mani Regmi
Orawee Kaewprasert
Angkana Chaiprasert
Virasakdi Chongsuvivatwong
Kiatichai Faksri
Universitas Sumatera Utara
University of Indonesia, RSUPN Dr. Cipto Mangunkusumo
Khon Kaen University
Faculty of Medicine, Siriraj Hospital, Mahidol University
Prince of Songkla University
Gandaki Medical College Teaching Hospital
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Jul-2020
Citation: Journal of medical microbiology. Vol.69, No.7 (2020), 1013-1019
Abstract: Introduction. Multidrug-resistant tuberculosis (MDR-TB) is a major public health problem globally, including in Indonesia. Whole-genome sequencing (WGS) analysis has rarely been used for the study of TB and MDR-TB in Indonesia.Aim. We evaluated the use of WGS for drug-susceptibility testing (DST) and to investigate the population structure of drug-resistant Mycobacterium tuberculosis in Java, Indonesia.Methodology. Thirty suspected MDR-TB isolates were subjected to MGIT 960 system (MGIT)-based DST and to WGS. Phylogenetic analysis was done using the WGS data. Results obtained using MGIT-based DST and WGS-based DST were compared.Results. Agreement between WGS and MGIT was 93.33 % for rifampicin, 83.33 % for isoniazid and 76.67 % for streptomycin but only 63.33 % for ethambutol. Moderate WGS-MGIT agreement was found for second-line drugs including amikacin, kanamycin and fluoroquinolone (73.33-76.67 %). MDR-TB was more common in isolates of the East Asian Lineage (63.3%). No evidence of clonal transmission of DR-TB was found among members of the tested population.Conclusion. Our study demonstrated the applicability of WGS for DST and molecular epidemiology of DR-TB in Java, Indonesia. We found no transmission of DR-TB in Indonesia.
ISSN: 14735644
Appears in Collections:Scopus 2020

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