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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/59905
Title: Albuminuria detection using graphene oxide-mediated fluorescence quenching aptasensor
Authors: Wireeya Chawjiraphan
Chayachon Apiwat
Khoonsake Segkhoonthod
Kiatnida Treerattrakoon
Preedee Pinpradup
Nuankanya Sathirapongsasuti
Prapasiri Pongprayoon
Patraporn Luksirikul
Patcharee Isarankura-Na-Ayudhya
Deanpen Japrung
University of Strathclyde
Kasetsart University
Thailand National Nanotechnology Center
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Thammasat University
Keywords: Biochemistry, Genetics and Molecular Biology;Health Professions
Issue Date: 1-Jan-2020
Citation: MethodsX. Vol.7, (2020)
Abstract: © 2020 A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of human serum albumin in urine. The limit of detection of the developed platform is 0.05 µg.mL−1 and the detection range is 0.1–600 µg.mL−1, which covers the albuminuria concentration range present in normal human urine and the urine of the patient with chronic kidney disease. This approach can be modified to measure albuminuria using a high-throughput quantification platform and portable point of care testing. In addition, the production cost for one reaction is cheaper than those for the standard automated method. Therefore, this aptasensor has significant potential for commercialization and public use. • Our protocol is customized by using the fluorescence quenching property of graphene oxide and specific binding property of human serum albumin aptamer to detect human serum albumin in urine sample • The limit of detection of our developed platform is 0.05 µg.mL−1 • The detection range of our aptasensor is 0.1–600 µg.mL−1
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/59905
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85094575071&origin=inward
ISSN: 22150161
Appears in Collections:Scopus 2020

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