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dc.contributor.authorYamaratee Jaisinen_US
dc.contributor.authorPiyanee Ratanachamnongen_US
dc.contributor.authorOrapin Wongsawatkulen_US
dc.contributor.authorAtthaboon Watthammawuten_US
dc.contributor.authorKittiya Malaniyomen_US
dc.contributor.authorSutthibhon Natewongen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherSrinakharinwirot Universityen_US
dc.date.accessioned2020-12-28T04:01:21Z-
dc.date.available2020-12-28T04:01:21Z-
dc.date.issued2020-12-15en_US
dc.identifier.citationLife Sciences. Vol.263, (2020)en_US
dc.identifier.issn18790631en_US
dc.identifier.issn00243205en_US
dc.identifier.other2-s2.0-85096213412en_US
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/60380-
dc.description.abstract© 2020 Elsevier Inc. The increase in intracellular reactive oxygen and nitrogen species plays a key role in ultraviolet B (UV-B)-induced inflammatory responses in the human skin. Piperine exhibits many pharmacological benefits. In the present study, the photoprotective effects and the possible underlying mechanisms of the anti-inflammatory effects of piperine on UV-B-irradiated keratinocytes were investigated. Piperine exerted strong, direct scavenging effects on DPPH radicals and exhibited free radical scavenging capabilities as demonstrated by the DCFH-DA and Griess assays. Consistent with these results, 10, 20, and 40 μM piperine pretreatments attenuated UV-B irradiation-induced keratinocyte cytotoxicity as reported by the resazurin assay. The highest concentration of piperine inhibited UV-B irradiation-induced cell apoptosis, as revealed by Hoechst 33342 staining. Moreover, we demonstrated the anti-inflammatory effects of piperine using western blot analysis, real-time PCR, and ELISA. Pretreatment with piperine suppressed the activation of phosphorylated p38, JNK, and AP-1 as well as the levels of COX-2/PGE2 and iNOS synthesis, while UV-B-irradiated cells triggered the induction of these signaling molecules. These results indicated that the inhibition of these inflammatory signaling pathways might play a key role in the regulation of the anti-inflammatory effects of piperine. In addition, piperine showed stronger anti-inflammatory effects than celecoxib which served as a positive control at the same concentration. All these results suggested that the anti-inflammatory properties of piperine protected keratinocytes from UV-B-induced damage, which might be due to its antioxidant properties. Therefore, piperine may be an effective therapeutic candidate compound for the treatment of UV irradiation-induced skin inflammation.en_US
dc.rightsMahidol Universityen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85096213412&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleAntioxidant and anti-inflammatory effects of piperine on UV-B-irradiated human HaCaT keratinocyte cellsen_US
dc.typeArticleen_US
dc.rights.holderSCOPUSen_US
dc.identifier.doi10.1016/j.lfs.2020.118607en_US
dc.identifier.urlhttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85096213412&origin=inwarden_US
Appears in Collections:Scopus 2020

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