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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/60458
Title: An integrative analysis of genome-wide methylation and expression in ameloblastoma: A pilot study
Authors: Monnat Pongpanich
Sirima Sanguansin
Sudaporn Kengkarn
Arkom Chaiwongkot
Boworn Klongnoi
Nakarin Kitkumthorn
Chulalongkorn University
Rangsit University
Mahidol University
Keywords: Dentistry
Issue Date: 1-Jan-2020
Citation: Oral Diseases. (2020)
Abstract: © 2020 Wiley Periodicals LLC Objective: DNA methylation regulates the expression of various genes involved in tumorigenesis. Ameloblastoma is a benign odontogenic jaw tumor. It is locally aggressive with a high level of recurrence. A delay in treatment can lead to severe facial disfigurement. To the best of our knowledge, this is the first integrated analysis of DNA methylation and gene expression in ameloblastoma with the aim to identify genes that may be regulated by DNA methylation. Materials and Methods: We used an Infinium MethylationEPIC array to measure genome-wide methylation and the Illumina HiSeq platform to obtain gene expression data in ameloblastoma tissues from five patients and dental follicles from three healthy subjects. An integration analysis was performed using City of Hope CpG Island Analysis Pipeline software. Results: We identified 25,255 differentially methylated CpG sites and 17 differentially methylated CpG islands; six of the islands were negatively correlated with the expression of BAIAP2, DUSP6, FGFR2, FOXF2, NID2, and PAK6. Pyrosequencing and immunostaining techniques were further used to validate FGFR2, NID2, and PAK6. Conclusions: This analysis identifies a group of novel genes that may be regulated by DNA methylation and will possibly lead to new insights into the pathology and invasion mechanism of ameloblastoma.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/60458
metadata.dc.identifier.url: https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85097030068&origin=inward
ISSN: 16010825
1354523X
Appears in Collections:Scopus 2020

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