Simple jQuery Dropdowns
Please use this identifier to cite or link to this item:
Title: Colorimetric reverse transcription loop-mediated isothermal amplification (RT-LAMP) as a visual diagnostic platform for the detection of the emerging coronavirus SARS-CoV-2
Authors: Kawin Nawattanapaiboon
Ekawat Pasomsub
Photchanathorn Prombun
Akanit Wongbunmak
Akarawit Jenjitwanich
Pantanat Mahasupachai
Purichaya Vetcho
Cholticha Chayrach
Natthapon Manatjaroenlap
Chonchanok Samphaongern
Treewat Watthanachockchai
Phonthanat Leedorkmai
Suwimon Manopwisedjaroen
Radeekorn Akkarawongsapat
Arunee Thitithanyanont
Matthew Phanchana
Watanalai Panbangred
Somchai Chauvatcharin
Toemsak Srikhirin
Faculty of Medicine, Ramathibodi Hospital, Mahidol University
Mahidol University
Zenostic Co.
Keywords: Biochemistry, Genetics and Molecular Biology;Chemistry;Environmental Science
Issue Date: 21-Jan-2021
Citation: Analyst. Vol.146, No.2 (2021), 471-477
Abstract: © 2021 The Royal Society of Chemistry. COVID-19, caused by the infection of SARS-CoV-2, has emerged as a rapidly spreading infection. The disease has now reached the level of a global pandemic and as a result a more rapid and simple detection method is imperative to curb the spread of the virus. We aimed to develop a visual diagnostic platform for SARS-CoV-2 based on colorimetric RT-LAMP with levels of sensitivity and specificity comparable to that of commercial qRT-PCR assays. In this work, the primers were designed to target a conserved region of the RNA-dependent RNA polymerase gene (RdRp). The assay was characterized for its sensitivity and specificity, and validated with clinical specimens collected in Thailand. The developed colorimetric RT-LAMP assay could amplify the target gene and enabled visual interpretation in 60 min at 65 °C. No cross-reactivity with six other common human respiratory viruses (influenza A virus subtypes H1 and H3, influenza B virus, respiratory syncytial virus types A and B, and human metapneumovirus) and five other human coronaviruses (MERS-CoV, HKU-1, OC43, 229E and NL63) was observed. The limit of detection was 25 copies per reaction when evaluated with contrived specimens. However, the detection rate at this concentration fell to 95.8% when the incubation time was reduced from 60 to 30 min. The diagnostic performance of the developed RT-LAMP assay was evaluated in 2120 clinical specimens and compared with the commercial qRT-PCR. The results revealed high sensitivity and specificity of 95.74% and 99.95%, respectively. The overall accuracy of the RT-LAMP assay was determined to be 99.86%. In summary, our results indicate that the developed colorimetric RT-LAMP provides a simple, sensitive and reliable approach for the detection of SARS-CoV-2 in clinical samples, implying its beneficial use as a diagnostic platform for COVID-19 screening.
ISSN: 13645528
Appears in Collections:Scopus 2021

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.