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dc.contributor.authorRuchuros Inkomlueen_US
dc.contributor.authorNoppadol Larbcharoensuben_US
dc.contributor.authorPatcharee Karnsombuten_US
dc.contributor.authorTassanee Lerksuthiraten_US
dc.contributor.authorRangsima Aroonrochen_US
dc.contributor.authorTassanee Lohnooen_US
dc.contributor.authorWanta Yingyongen_US
dc.contributor.authorPitak Santaniranden_US
dc.contributor.authorLalana Sansophaen_US
dc.contributor.authorTheerapong Krajaejunaen_US
dc.contributor.otherMahidol University. Faculty of Medicine, Ramathibodi Hospital. Department of Pathologyen_US
dc.contributor.otherMahidol University. Faculty of Medicine, Ramathibodi Hospital. Research Centeren_US
dc.contributor.otherChulalongkorn University. Faculty of Medicine. Department of Pathologyen_US
dc.contributor.otherMahidol University. Faculty of Science. Multidisciplinary Unit, Molecular Medicine Programen_US
dc.identifier.citationJournal of Clinical Microbiology. Vol.54, No.1 (Jan 2016), 43-48en_US
dc.description.abstractPythiosis is an emerging and life-threatening infectious disease of humans and animals living in tropical and subtropical countries and is caused by the fungus-like organism Pythium insidiosum. Antifungals are ineffective against this pathogen. Most patients undergo surgical removal of the infected organ, and many die from advanced infections. Early and accurate diagnosis leads to prompt management and promotes better prognosis for affected patients. Immunohistochemical assays (IHCs) have been developed using rabbit antibodies raised against P. insidiosum crude extract, i.e., culture filtrate antigen (CFA), for the histodiagnosis of pythiosis, but cross-reactivity with pathogenic fungi compromises the diagnostic performance of the IHC. Therefore, there is a need to improve detection specificity. Recently, the elicitin protein, ELI025, was identified in P. insidiosum, but it was not identified in other human pathogens, including true fungi. The ELI025-encoding gene was successfully cloned and expressed as a recombinant protein in Escherichia coli. This study aims to develop a new IHC using the rabbit anti-ELI025 antibody (anti-ELI) and to compare its performance with the previously reported anti-CFA-based IHC. Thirty-eight P. insidiosum histological sections stained positive by anti-ELI-based and anti-CFA-based IHCs indicating 100% detection sensitivity for the two assays. The anti-ELI antibody stained negative for all 49 negative-control sections indicating 100% detection specificity. In contrast, the anti-CFA antibody stained positive for one of the 49 negative controls (a slide prepared from Fusarium-infected tissue) indicating 98% detection specificity. In conclusion, the anti-ELI based IHC is sensitive and specific for the histodiagnosis of pythiosis and is an improvement over the anti-CFA-based assay.en_US
dc.rightsMahidol Universityen_US
dc.subjectImmunohistochemical assaysen_US
dc.titleDevelopment of an Anti-Elicitin Antibody-Based Immunohistochemical Assay for Diagnosis of Pythiosisen_US
dc.typeResearch Articleen_US
dc.rights.holderAmerican Society for Microbiologyen_US
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