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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/61245
Title: Effect of Clausena harmandiana root extract, Garcinia mangostana Linn rind extract and whole Andrographis paniculata extracts on growth of Pythium insidiosum
Authors: Nujarin Jongruja
Tassanee Lohnoo
Wanta Yingyong
Yothin Kumsang
Thidarat Rujirawat
Tassanee Lerksuthirat
Kovit Pattanapanyasat
King Mongkut’s University of Technology Thonburi. Faculty of Science. Department of Microbiology
Mahidol University. Faculty of Medicine, Ramathibodi Hospital. Research Center
Mahidol University. Faculty of Medicine, Ramathibodi Hospital. Department of Radiology
Mahidol University. Faculty of Science. Multidisciplinary Unit
Mahidol University. Faculty of Medicine, Siriraj Hospital
Keywords: Pythium insidiosum;Pythiosis;Garcinia mangostana Linn;Andrographis paniculata;Clausena harmandiana
Issue Date: 2016
Abstract: Pythium insidiosum is categorized into a group of oomycetes, that are filamentous eukaryotic microorganisms. P. insidiosum is the only species reported e ie a human and animal pathogen. The disease caused by the pathogen is called pythiosis. Pythiosis is one of the life-threatening infectious diseases, because it may lead to the loss of infected tissues, organs or mortality. Although the disease severity is relatively high, effective and suitable medicines remain unavailable. This study aimed to evaluate the effective inhibitory activity of extracts from Garcinia mangostana Linn fruit rind and Andrographis paniculata against various isolates of P. insidiosum and their inhibitory activity compared to the root extract of Clausena harmandiana. The crude extracts were tested for antimicrobial aaeahaey against twenty clinical and environmental isolates of P. insidiosum by determination of their minimum inhibitory concentration (MIC) values by agar well diffusion and disc diffusion assays. The present study indicated that extracts from the garcinia fruit rind had inhibitory activity against clinical isolates of P. insidiosum. Every isolate of P. insidiosum was inhibited by 2 mg/ml of the mangosteen fruit rind extract, whereas whole Andrographis paniculata and Clausena harmandiana root extracts exhibited inhibitory activity against all isolates at 5 mg/ml and 10 mg/ml, respectively. Results from the agar well diffusion and disc diffusion assays also indicated that the extracts from Garcinia mangostana Linn fruit rind might be effective and were promising for future studies as one of the medicines for treating pythiosis. The ethanolic extract of the mangosteen rind had a slightly higher phenolic content than the aqueous extract. Therefore, the ethanolic extract from G. mangostana Linn fruit rind was considered to be appropriate for further studies for drug discovery and development for treatment of pythiosis disease.
Description: The 5th International Biochemistry and Molecular Biology Conference 2016 (BMB2016). Departments of Molecular Biology and Bioinformatics and the Department of Biochemistry, Faculty of Science, Prince of Songkla University. May 26 to 27, 2016. p.439-442.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/61245
Appears in Collections:RA-Proceeding Document

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