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Title: cDNA expression library and immunoscreening of thai paragonimus heterotremus
Authors: Kanokkarn Pothong
กนกกาญจน์ โพธิ์ทอง
Paron Dekumyoy
พารณ ดีคำย้อย
Thareerat Kalambaheti
ธารีรัตน์ กะลัมพะเหติ
Dorn Watthanakulpanich
ดร วัฒนกุลพานิชย์
Chalit Komalmisra
ชลิต โกมลมิศร์
Timothy P Yoshino
Mahidol University. Faculty of Tropical Medicine. Department of Helminthology
Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology
Keywords: Paragonimus heterotemus;cDNA
Issue Date: 2014
Abstract: A cDNA expression library of Paragonimus heterotremus, the main causative agent of human paragonimiasis in Thailand, was performed to produce recombinant antigens. To identify the antigenic clones, immunized sera were tested using immunoscreening. Ten positive clones were selected, and the inserted genes partially sequenced. The sequencing data were analyzed using BLASTp searches. Six clones were found to contain a gene insert that encoded a protein similar to that found in helminthic worms (Clonorchis sinensis, Schistosoma mansoni, Schistosoma japonicum, Echinococcus multilocularis, Brugia malayi), two clones similar to Toxoplasma gondii, while two more clones were shown to encode proteins similar to that of Plasmodium chabaudi chabaudiand Microcystis spp. (uncultured). The clones will be further studied in term of protein expression through recombinant antigen evaluation using human paragonimiasis and other parasitic infection sera.
Description: Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 278.
Appears in Collections:TM-Proceeding Document

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