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dc.contributor.authorK Chaichanaen_US
dc.contributor.authorJitra Waikagulen_US
dc.contributor.authorจิตรา ไวคกุลen_US
dc.contributor.authorMalinee Thairungrojen_US
dc.contributor.authorมาลินี ไทยรุ่งโรจน์en_US
dc.contributor.authorParon Dekumyoyen_US
dc.contributor.authorพารณ ดีคำย้อยen_US
dc.contributor.authorChalit Komalamisraen_US
dc.contributor.authorชลิต โกมลมิศร์en_US
dc.contributor.authorViroj Kitikoonen_US
dc.contributor.authorวิโรจน์ กิติคุณen_US
dc.contributor.otherMahidol University. Faculty of Tropical Medicine. Department of Helminthologyen_US
dc.contributor.otherMahidol University. Faculty of Tropical Medicine. Department of Social and Environmental Medicineen_US
dc.date.accessioned2016-01-18T06:45:04Z
dc.date.accessioned2021-08-30T15:35:45Z-
dc.date.available2016-01-18T06:45:04Z
dc.date.available2021-08-30T15:35:45Z-
dc.date.created2016-01-18
dc.date.issued2001
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/63349-
dc.descriptionJoint International Tropical Medicine Meeting 2001: Century Pard Hotel, Bangkok, Thailand 8-10 August 2001: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2001. p.219en
dc.description.abstractThis study aimed to develop a sensitive and specific immunodiagnosis method for Opisthorchis viverrini infection using purified Bithynia goniomphalos snails as a cocktail antigen by gel filtration chromatography and an eluted antigen by SDS-polyacrylamide gel electrophoresis and electroelution method. Effectiveness and specificity in the serological diagnosis of opisthorchiasis using these wwo prepared antigens were tested by indirect ELISA against sera of 61 cases with opisthorchiasis, 125 cases with other parasitic infections and 30 normal healthy sera. The sensitivity, specificity,, positive and negative predictive values of cocktail antigen were 95.1%, 79.4%, 64.4% and 97.6%, respectively, at the cut off OD of 0.786. Cross reactions were observed with trichuriasis (1/7), trichinellosis (5/10), hookworm infection (5/9), angiostrongvliasis (2/9), toxocariasis (1/5), ascariasis (1/8), filariasis (1/6), taeniasis (1/9), hymenolepiasis (1/4), hydatidosis (2/3 and gnathostomiasis (7/9). No cross-reaction was found with normal healthy sera. The sensitivity, specificity, positive and negative predictive values of eluted antigen (53 kDa) were 96.7% , 92.9%, 84.3, and 98.6%, respectively, at the cut off OD of 0.814. Cross reactions were observed with strongyloidiasis (4/10), angiostrongbliasis (1/9), gnathostomiasis (3/9), hymenolepiasis (1/4) and paragonimiasis (2/4). No cross-reaction was found with normal healthy sera. Further studies on improvement of cocktail and eluted antigen preparation may provide a better specific diagnosis of opisthorchiasis.en_US
dc.language.isoenen_US
dc.rightsMahidol Universityen_US
dc.subjectOpisthorchis viverrinien_US
dc.subjectParasiticen_US
dc.titleDiagnosis of human opisthorchiasis with cocktail and eluted Bithynia goniomphalos snail antigens by indirect elisaen_US
dc.typeProceeding Posteren_US
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