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Title: The role of cycle inhibiting factor in burkholderia pseudomallei
Authors: Pornpan Pumirat
พรพรรณ ภูมิรัตน์
Onrapak Reamtong
อรภัค เลี่ยมทอง
Narisara Chantratita
นริศรา จันทราทิตย์
Sunee Korbsrisate
Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology
Mahidol University. Faculty of Tropical Medicine. Department of Molecular Tropical Medicine and Genetics
Mahidol University. Faculty of Tropical Medicine. Mahidol Oxford Research Unit
Keywords: B. pseudomallei;Cycle-inhibiting factor;Pathogenesis
Issue Date: 2014
Abstract: Burkholderia pseudomallei, a causative agent of melioidosis, harbor several virulence factors crucial for its pathogenesis. A cycle-inhibiting factor (Cif) is one of bacterial effector molecules produced by B. pseudomallei, however the role of this protein is still unclear. In our previous study, we constructed a B. pseudomallei cif mutant to explore the function of Cif in B. pseudomallei infection. Infection of U937 macrophage with B. pseudomallei revealed that host cell contact was required for the secretion of Cif. Interestingly, Cif was secreted in the host cell in a manner dependent on the Bsa type III secretion system. Moreover, the responsibility of Cif is likely important for B. pseudomallei pathogenesis as we found B. pseudomallei cif mutant showed significant defects in abilities to induce plaque formation and cytotoxicity of HeLa cells when compared to the wild type. We further investigated the host protein expression profiles of infected HeLa cells using mass spectrometry. Investigation by this technique identified 97 up-regulated and 166 down-regulated proteins that significantly altered in HeLa cells infected with chbP mutant compared with that of B. pseudomallei wild type strain. Among these, some are associated with metabolic process, catalytic activity and signaling pathways. Thus, based upon our findings, we indicate a requirement of Cif that deliver via Bsa apparatus in B. pseudomallei infection, and the role of Cif might be involved with regulation of the host cell expression to alter the host cell functions which benefit for the pathogenesis of B. pseudomallei.
Description: Joint International Tropical Medicine Meeting 2014: 3D perspectives on tropical medicine: drivers, diversity and determination the 8th seminar on food-and water-borne parasitic zoonoses: 2-4 December 2014: Centara Grand Bangkok Convention Center at Central World, Bangkok, Thailand. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2014. p. 211.
Appears in Collections:TM-Proceeding Document

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