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|Title:||Rare or unrecognized evidence of human dirofilariasis in Thailand: possible immonoblot dianosis of one Thai patient|
Mahidol University. Faculty of Tropical Medicine. Department of Helminthology
|Keywords:||Dirofilaria immitis female;IgGimmunolot|
|Abstract:||Human dirofilariasis is a rare or unrecognized zoonotic disease but a high incidence has been reported in domestic dogs in the North of Thailand, and unreported in other parts. This disease has been widely reported in canine and human cases in Australia, Europe, and the Americas. Many species of mosquitoes carry the infective larva of Dirofilaria immitis, which are also present in the Bangkok metropolitan area. Cases usually present as symptomatic, but symptomatic patients commonly show chest pain, cough, or hemoptysis. A peripheral coin lesion in the lung is the typical characteristic of human dirofilariasis, but this pulmonary nodule may simulate primary or metastatic lung tumor. One infection of a Thai patient with D. immitis was presented by a cyst in the lung; a series of cystic sections showed particular characteristics of a dog’s heartworm. This study aimed to sero-differentiate one human dirofilariasis from other infections using female and male crude antigens. Serum antibodies were derived from nematodiases, cestodiases, trematodiases, protozoan infections, and healthy controls. The antibody of this patient reacted with 24.5 kDa female crude antigen, which does not react with antibodies of other serum samples and healthy controls. This antigen reacted with antibody against one Myanmar patient infected with Wuchereria bancrofti microfilariae. The antigens of male crude extract reacted with antibody of this case at 32 and 29 kDa, of which only 32 kDa reacted weakly with antibodies to some cases of cystic echinococcosis, sparganosis, paragonimiasis heterotremus, and minute intestinal fluke. The 29 kDa male crude extract was of interest for its diagnostic bands, but only one healthy control from Australia cross-reacted with this antigen. Serum antibody of this case showed negative to ICT (immunochromatographic tests to detect Bancroftian filariasis) and high titers with individual female and male crude antigens of D. immitis at 1:6,400 by IgG-ELISA. This research found two interesting antigens, 24.5 kDa female worm extract, and 29 kDa male worm extract, as differential diagnoses. Further study should conclude many cases of human dirofilariasis, and immunoblot testing should be further refined for greater specificity the 24.5 and 29 kDa bands for human dirofilariasis, and/or to produce ELISA-screening tests using a combination of both antigens with many lung infections and other diseases.|
|Description:||Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.182.|
|Appears in Collections:||TM-Proceeding Document|
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