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dc.contributor.authorKrasae Kanakupten_US
dc.contributor.authorกระแสร์ คณาคุปต์en_US
dc.contributor.authorMalinee Thairungroj Anantaphrutien_US
dc.contributor.authorมาลินี ไทยรุ่งโรจน์ อนันต์พฤกษ์en_US
dc.contributor.authorTran Thi Hongen_US
dc.contributor.authorJitra Waikagulen_US
dc.contributor.authorจิตรา ไวคกุลen_US
dc.contributor.authorParon Dekumyoyen_US
dc.contributor.authorพารณ ดีคำย้อยen_US
dc.contributor.authorWichit Rojekitikhunen_US
dc.contributor.authorวิชิต โรจน์กิตติคุณen_US
dc.contributor.authorYaowapa Maneeraten_US
dc.contributor.authorSuwannee Nithiuthaien_US
dc.contributor.otherMahidol University. Faculty of Tropical Medicine. Department of Helminthologyen_US
dc.date.accessioned2015-12-18T04:19:00Z
dc.date.accessioned2021-08-30T15:42:21Z-
dc.date.available2015-12-18T04:19:00Z
dc.date.available2021-08-30T15:42:21Z-
dc.date.created2015-12-18
dc.date.issued2005
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/63383-
dc.descriptionJoint International Tropical Medicine Meeting 2005: The Grand Hotel, Bangkok, Thailand 30 November – 2 December 2005: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2005. p.182.en
dc.description.abstractThis study aimed to evaluate antigens prepared from adult Toxocara canis worms against human toxocariasis. Individual female and male worms were provided as crude somatic extract, partially somatic extract by Sephacryl S-200 chromatography and excretory-secretory (ES) products. All antigens were evaluated for sensitivity and specificity by ELISA and Western blot. ELISA using crude somatic antigen showed 97.6% sensitivity, with poor specificity. The peak 1 sensitivity of female and male somatic antigens was 95.1% and 92.7%, respectively, with specificity of 46.7% and 39.8%, respectively. The male peak 2 antigens showed 51.2% sensitivity and 67.8% specificity. The female and male ES antigens showed 95.0% and 87.8% sensitivity, respectively, and with low specificity (42.6% and 39.9%, respectively). Immunoblot using female crude somatic antigen revealed 52 kDa, which was not found in negative control or heterologous sera. However it was found in only 4 of 15 toxocariasis sera. Female 33 kDa ES antigen was found in 7 of 15 toxocariasis sera, but it cross-reacted with gnathostomiasis, angiostrongyliasis, strongyloidiasis and capillariasis. Male crude somatic and ES antigens did not demonstrate any diagnostic band among the human sera tested. All antigens in this study needed more advanced purification techniques to obtain higher specificity.en_US
dc.language.isoenen_US
dc.rightsMahidol Universityen_US
dc.subjectAntigensen_US
dc.subjectToxocariasisen_US
dc.titleEvaluation of excretory-secretory and partially purified antigens of adult toxocara canis against human toxocariasis by elisa and immunobloten_US
dc.typeProceeding Posteren_US
Appears in Collections:TM-Proceeding Document

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