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Title: Observation of intracellular protozoan development by tissue culture on cover glasses and detection by fresh observation and staining methods
Authors: Chutatip Siripanth
จุฑาทิพ ศิริพันธุ์
Benjanee Punpoowong
เบญจนี พันธ์ภูวงศ์
Pornsawan Ammarapal
พรสวรรค์ อัมระปาล
Niramol Thima
Mahidol University. Faculty of Tropical Medicine. Department of Protozoology
Mahidol University. Faculty of Tropical Medicine. Department of Tropical Pathology
Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology
Keywords: Parasite
Issue Date: 2004
Abstract: Isospora belli, Cryptosporidium parvum sporozoites and Microsporidial spores were infected into culture cells. The developmental stages of these intracellular protozoa were described after the fresh observation and staining of infected cells on cover glasses. Paraffin tissue section was performed and compared to cover glass staining. Fresh observation was more advantages for the observation of the movement of I. belli merozoites but the development of merogony of Cryptosporidium could not be notified by fresh observation. The merogony stages of I. belli from paraffin tissue sections were not clear, compared to cover glass staining but the trophozoites of Cryptosporidium were clearly demonstrated by paraffin tissue sections. The typical characters of belt-like stripes of Microsporidial spores from culture were clearly observed from cover glass staining. In conclusion, cover glass staining could be used for an alternative method for the detection of intracellular protozoa from cultures. However, the properstaining methodsshould be selected for each protozoan parasite.
Description: Joint International Tropical Medicine Meeting 2004: Ambassador Hotel, Thailand 29 November-1 December 2004: abstract. Bangkok: Faculty of Tropical Medicine, Mahidol University; 2004. p.224.
Appears in Collections:TM-Proceeding Document

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