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dc.contributor.authorKesinee Chotivanichen_US
dc.contributor.authorเกศินี โชติวานิชen_US
dc.contributor.authorMathirut Mungthinen_US
dc.contributor.authorมฑิรุทธ มุ่งถิ่นen_US
dc.contributor.authorRonnatrai Ruengweerayuthen_US
dc.contributor.authorRachanee Udomsangpetcen_US
dc.contributor.authorรัชนีย์ อุดมแสงเพ็ชรen_US
dc.contributor.authorDondorp, Arjen M.en_US
dc.contributor.authorPratap Singhasivanonen_US
dc.contributor.authorประตาป สิงหศิวานนท์en_US
dc.contributor.authorSasithon Pukrittayakameeen_US
dc.contributor.authorศศิธร ผู้กฤตยาคามี
dc.contributor.authorWhite, Nicholas J.
dc.contributor.otherMahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit
dc.contributor.otherMahidol University. Faculty of Science. Department of Pathobiology
dc.date.accessioned2012-12-20T07:42:53Z
dc.date.accessioned2016-09-22T06:00:10Z-
dc.date.available2012-12-20T07:42:53Z
dc.date.available2016-09-22T06:00:10Z-
dc.date.copyright2012
dc.date.created2012-12-20
dc.date.issued2012-05-28
dc.identifier.citationChotivanich K, Mungthin M, Ruengweerayuth R, Udomsangpetch R, Dondorp AM, Singhasivanon P, et al. The effects of serum lipids on the in vitro activity of lumefantrine and atovaquone against Plasmodium falciparum. Malar J. 2012 May 28;11:177.en_US
dc.identifier.issn1475-2875 (electronic)
dc.identifier.urihttp://repository.li.mahidol.ac.th/dspace/handle/123456789/709-
dc.description.abstractBACKGROUND: Lumefantrine and atovaquone are highly lipophilic anti-malarial drugs. As a consequence absorption is increased when the drugs are taken together with a fatty meal, but the free fraction of active drug decreases in the presence of triglyceride-rich plasma lipoproteins. In this study, the consequences of lipidaemia on anti-malarial drug efficacy were assessed in vitro. METHODS: Serum was obtained from non-immune volunteers under fasting conditions and after ingestion of a high fat meal and used in standard Plasmodium falciparum in-vitro susceptibility assays. Anti-malarial drugs, including lumefantrine, atovaquone and chloroquine in five-fold dilutions (range 0.05 ng/ml-1 ug/mL) were diluted in culture medium supplemented with fasting or post-prandial 10% donor serum. The in-vitro drug susceptibility of parasite isolates was determined using the ³H-hypoxanthine uptake inhibition method and expressed as the concentration which gave 50% inhibition of hypoxanthine uptake (IC₅₀). RESULTS: Doubling plasma triglyceride concentrations (from 160 mg/dL to 320 mg/dL), resulted in an approximate doubling of the IC₅₀ for lumefantrine (191 ng/mL to 465 ng/mL, P < 0.01) and a 20-fold increase in the IC₅₀ for atovaquone (0.5 ng/mL to 12 ng/ml; P < 0.01). In contrast, susceptibility to the hydrophilic anti-malarial chloroquine did not change in relation to triglyceride content of the medium. CONCLUSIONS: Lipidaemia reduces the anti-malarial activity of lipophilic anti-malarial drugs. This is an important confounder in laboratory in vitro testing and it could have therapeutic relevance.en_US
dc.language.isoenen_US
dc.rightsMahidol Universityen_US
dc.subjectAnti-malarial drugsen_US
dc.subjectIn vitro-susceptibilityen_US
dc.subjectMalariaen_US
dc.subjectOpen Access articleen_US
dc.titleThe effects of serum lipids on the in vitro activity of lumefantrine and atovaquone against Plasmodium falciparumen_US
dc.typeResearch Articleen_US
dc.rights.holderBioMed Centralen_US
dc.contributor.correspondenceWhite, Nicholas J.en_US
dc.date.accepted2012-04-25
dc.identifier.doi10.1186/1475-2875-11-177
dc.identifier.urlhttp://www.malariajournal.com/content/pdf/1475-2875-11-177.pdf
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