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dc.contributor.authorSteenkeste, Nicolasen_US
dc.contributor.authorDillies, Marie-Agnèsen_US
dc.contributor.authorKhim, Nimolen_US
dc.contributor.authorSismeiro, Odileen_US
dc.contributor.authorChy, Sophyen_US
dc.contributor.authorLim, Pharathen_US
dc.contributor.authorCrameri, Andreasen_US
dc.contributor.authorBouchier, Christianeen_US
dc.contributor.authorMercereau-Puijalon, Odileen_US
dc.contributor.authorBeck, Hans-Peteren_US
dc.contributor.authorMallika Imwongen_US
dc.contributor.authorมัลลิกา อิ่มวงศ์en_US
dc.contributor.authorDondorp, Arjen M.en_US
dc.contributor.authorSocheat, Duongen_US
dc.contributor.authorRogier, Christopheen_US
dc.contributor.authorCoppée, Jean- Yvesen_US
dc.contributor.authorAriey, Frédéricen_US
dc.contributor.otherMahidol University. Faculty of Tropical Medicine
dc.identifier.citationSteenkeste N, Dillies MA, Khim N, Sismeiro O, Chy S, Lim P, et al. FlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance. Malar J. 2009 Oct 15;8:229.en_US
dc.identifier.issn1475-2875 (electronic)
dc.description.abstractBACKGROUND: A number of molecular tools have been developed to monitor the emergence and spread of anti-malarial drug resistance to Plasmodium falciparum. One of the major obstacles to the wider implementation of these tools is the absence of practical methods enabling high throughput analysis. Here a new Zip-code array is described, called FlexiChip, linked to a dedicated software program, which largely overcomes this problem. METHODS: Previously published microarray probes detecting single-nucleotide polymorphisms (SNP) associated with parasite resistance to anti-malarial drugs (ResMalChip) were adapted for a universal microarray FlexiChip format. To evaluate the overall sensitivity of the FlexiChip package (microarray + software), the results of FlexiChip were compared to ResMalChip microarray, using the same extension probes and with the same PCR products. In both cases, sequence results were used as gold standard to calculate sensitivity and specificity. FlexiChip results obtained with a set of field isolates were then compared to those assessed in an independent reference laboratory. RESULTS: The FlexiChip package gave results identical to the ResMalChip results in 92.7% of samples (kappa coefficient 0.8491, with a standard error 0.021) and had a sensitivity of 95.88% and a specificity of 97.68% compared to the sequencing as the reference method. Moreover the method performed well compared to the results obtained in the reference laboratories, with 99.7% of identical results (kappa coefficient 0.9923, S.E. 0.0523). CONCLUSION: Microarrays could be employed to monitor P. falciparum drug resistance markers with greater cost effectiveness and the possibility for high throughput analysis. The FlexiChip package is a promising tool for use in poor resource settings of malaria endemic countries.en_US
dc.rightsMahidol Universityen_US
dc.subjectDrug resistanceen_US
dc.subjectMicroarray analysisen_US
dc.subjectParasitic sensitivity testsen_US
dc.subjectPlasmodium falciparumen_US
dc.subjectPolymorphism, single nucleotideen_US
dc.subjectSensitivity and Specificityen_US
dc.subjectOpen Access articleen_US
dc.titleFlexiChip package: an universal microarray with a dedicated analysis software for high-thoughput SNPs detection linked to anti-malarial drug resistance.en_US
dc.rights.holderBioMed Centralen_US
dc.contributor.correspondenceSteenkeste, Nicolasen_US
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