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Title: Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei
Authors: Direk Limmathurotsakul
ดิเรก ลิ้มมธุรสกุล
Dance, David A. B.
Vanaporn Wuthiekanun
Kaestli, Mirjam
Mayo, Mark
Warner, Jeffrey
Wagner, David M.
Apichai Tuanyok
Wertheim, Heiman
Cheng, Tan Yoke
Mukhopadhyay, Chiranjay
Puthucheary, Savithiri
Day,Nicholas P. J.
Steinmetz, Ivo
Currie, Bart J.
Peacock, Sharon J.
Mahidol University. Faculty of Tropical Medicine. Department of Tropical Hygiene
Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit
Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology
Direk Limmathurotsakul
Keywords: Burkholderia pseudomallei;Environmental microbiology;Guidelines as topic;Open Access article
Issue Date: Mar-2013
Citation: Limmathurotsakul D, Dance DA, Wuthiekanun V, Kaestli M, Mayo M, Warner J, et al. Systematic review and consensus guidelines for environmental sampling of Burkholderia pseudomallei. PLoS Negl Trop Dis. 2013;7(3):e2105.
Abstract: BACKGROUND: Burkholderia pseudomallei, a Tier 1 Select Agent and the cause of melioidosis, is a Gram-negative bacillus present in the environment in many tropical countries. Defining the global pattern of B. pseudomallei distribution underpins efforts to prevent infection, and is dependent upon robust environmental sampling methodology. Our objective was to review the literature on the detection of environmental B. pseudomallei, update the risk map for melioidosis, and propose international consensus guidelines for soil sampling. METHODS/PRINCIPAL FINDINGS: An international working party (Detection of Environmental Burkholderia pseudomallei Working Party (DEBWorP)) was formed during the VIth World Melioidosis Congress in 2010. PubMed (January 1912 to December 2011) was searched using the following MeSH terms: pseudomallei or melioidosis. Bibliographies were hand-searched for secondary references. The reported geographical distribution of B. pseudomallei in the environment was mapped and categorized as definite, probable, or possible. The methodology used for detecting environmental B. pseudomallei was extracted and collated. We found that global coverage was patchy, with a lack of studies in many areas where melioidosis is suspected to occur. The sampling strategies and bacterial identification methods used were highly variable, and not all were robust. We developed consensus guidelines with the goals of reducing the probability of false-negative results, and the provision of affordable and 'low-tech' methodology that is applicable in both developed and developing countries. CONCLUSIONS/SIGNIFICANCE: The proposed consensus guidelines provide the basis for the development of an accurate and comprehensive global map of environmental B. pseudomallei.
ISSN: 1935-2727 (printed)
Appears in Collections:TM-Article

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