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Title: DNA barcode identification of freshwater snails in the family Bithyniidae from Thailand.
Authors: Jutharat Kulsantiwong
จุฑารัตน์ กุลสันติวงศ์
Sattrachai Prasopdee
ศาสตราชัย ประสพดี
Jiraporn Ruangsittichai
จิราภรณ์ เรืองสิทธิชัย
Wipaporn Ruangjirachuporn
วิภาภรณ์ เรืองจิระชูพร
Thidarut Boonma
ธิดารัตน์ บุญมาศ
Vithoon Viyanan
วิฑูรย์ ไวยนันท์
Pierossi, Paola
Hebert, Paul D. N.
Smarn Tesan
สมาน เทศนา
Mahidol University. Faculty of Tropical Medicine. Department of Medical Entomology
Smarn Tesan
Keywords: Animals;DNA, Mitochondrial;Genetic variation;Open Access article
Issue Date: 4-Nov-2013
Citation: Kulsantiwong J, Prasopdee S, Ruangsittichai J, Ruangjirachuporn W, Boonmars T, Viyanant V, et al. DNA barcode identification of freshwater snails in the family Bithyniidae from Thailand. PLoS One. 2013 Nov 4;8(11):e79144.
Abstract: Freshwater snails in the family Bithyniidae are the first intermediate host for Southeast Asian liver fluke (Opisthorchis viverrini), the causative agent of opisthorchiasis. Unfortunately, the subtle morphological characters that differentiate species in this group are not easily discerned by non-specialists. This is a serious matter because the identification of bithyniid species is a fundamental prerequisite for better understanding of the epidemiology of this disease. Because DNA barcoding, the analysis of sequence diversity in the 5' region of the mitochondrial COI gene, has shown strong performance in other taxonomic groups, we decided to test its capacity to resolve 10 species/ subspecies of bithyniids from Thailand. Our analysis of 217 specimens indicated that COI sequences delivered species-level identification for 9 of 10 currently recognized species. The mean intraspecific divergence of COI was 2.3% (range 0-9.2 %), whereas sequence divergences between congeneric species averaged 8.7% (range 0-22.2 %). Although our results indicate that DNA barcoding can differentiate species of these medically-important snails, we also detected evidence for the presence of one overlooked species and one possible case of synonymy.
ISSN: 1932-6203 (electronic)
Appears in Collections:TM-Article

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