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Title: Latticed gold nanoparticle conjugation via monomeric streptavidin in lateral flow assay for detection of autoantibody to interferon-gamma
Authors: Weeraya Thongkum
Umpa Yasamut
Koollawat Chupradit
Supachai Sakkhachornphop
Jiraprapa Wipasa
Kanokporn Sornsuwan
On Anong Juntit
Rawiwan Pornprasit
Wanwisa Thongkamwitoon
Jirapan Chaichanan
Jaruwan Khaoplab
Chonnikarn Chanpradab
Watchara Kasinrerk
Chatchai Tayapiwatana
Mahidol University
Chiang Mai University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 1-Jun-2021
Citation: Diagnostics. Vol.11, No.6 (2021)
Abstract: Adult-onset immunodeficiency syndrome (AOID) patients with autoantibodies (autoAbs) against interferon-gamma (IFN-γ) generally suffer from recurrent and recalcitrant disseminated non-tuberculous mycobacterial diseases. Since the early stages of AOID do not present specific symptoms, diagnosis and treatment of the condition are not practical. A simplified diagnostic method for differentiating AOID from other immunodeficiencies, such as HIV infection, was cre-ated. Anti-IFN-γ is generally identified using enzyme-linked immunosorbent assay (ELISA), which involves an instrument and a cumbersome process. Recombinant IFN-γ indirectly conjugated to colloidal gold was used in the modified immunochromatographic (IC) strips. The biotinylated-IFN-γ was incorporated with colloidal-gold-labeled 6HIS-maltose binding protein-monomeric streptav-idin (6HISMBP-mSA) and absorbed at the conjugate pad. The efficacy of the IC strip upon applying an anti-IFN-γ autoAb cut-off ELISA titer of 2500, the sensitivity and specificity were 84% and 90.24%, respectively. When a cut-off ELISA titer of 500 was applied, the sensitivity and specificity were 73.52% and 100%, respectively.
ISSN: 20754418
Appears in Collections:Scopus 2021

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