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|Title:||Evaluation of a latex agglutination assay for the identification of Burkholderia pseudomallei and Burkholderia mallei.|
|Authors:||Duval, Brea D.|
Elrod, Mindy G.
Gee, Jay E.
Hoffmaster, Alex R.
Mahidol University. Faculty of Tropical Medicine. Department of Microbiology and Immunology.
Mahidol University. Faculty of Tropical Medicine. Mahidol-Oxford Tropical Medicine Research Unit.
Mahidol University. Faculty of Tropical Medicine.Department of Tropical Hygiene.
Hoffmaster, Alex R.
|Keywords:||Burkholderia mallei;Burkholderia pseudomallei;Open Access article|
|Citation:||Duval BD, Elrod MG, Gee JE, Chantratita N, Tandhavanant S, Limmathurotsakul D. et al. Evaluation of a latex agglutination assay for the identificationof Burkholderia pseudomallei and Burkholderia mallei. Am J Trop Med Hyg. 2014 Jun;90(6):1043-6.|
|Abstract:||Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested.|
|Appears in Collections:||TM-Article|
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