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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/929
Title: Altered ATP release and metabolism in dorsal root ganglia of neuropathic rats.
Authors: Somsak Mitrirattanakul
Yoshizo Matsuka
Takeshi Ono
Hirotate Iwase
Kevin S Omoto
Ting Cho
Yan Yan N Lam
Bradley Snyder
สมศักดิ์ ไมตรีรัตนะกุล
Igor Spigelman
Keywords: Neuropathic rats;Dorsal root ganglia;Adenosine 5'-triphosphate;Open Access article
Issue Date: 2008
Citation: Matsuka Y, Ono T, Iwase H, Mitrirattanakul S, Omoto KS, Cho T, et al. Altered ATP release and metabolism in dorsal root ganglia of neuropathic rats. Molecular Pain. 2008; 4:66.
Abstract: Background: Adenosine 5'-triphosphate (ATP) has a ubiquitous role in metabolism and a major role in pain responses after tissue injury. We investigated the changes in basal and KCl-evoked ATP release from rat dorsal root ganglia (DRG) after peripheral neuropathy induction by unilateral sciatic nerve entrapment (SNE). Results: After SNE, rats develop long-lasting decreases in ipsilateral hindpaw withdrawal thresholds to mechanical and thermal stimulation. At 15–21 days after neuropathy induction, excised ipsilateral L4-L5 DRG display significantly elevated basal extracellular ATP levels compared to contralateral or control (naive) DRG. However, KCl-evoked ATP release is no longer observed in ipsilateral DRG. We hypothesized that the differential SNE effects on basal and evoked ATP release could result from the conversion of extracellular ATP to adenosine with subsequent activation of adenosine A1 receptors (A1Rs) on DRG neurons. Adding the selective A1R agonist, 2-chloro-N6-cyclopentyladenosine (100 nM) significantly decreased basal and evoked ATP release in DRG from na?ve rats, indicating functional A1R activation. In DRG ipsilateral to SNE, adding a selective A1R antagonist, 8-cyclopentyl-1,3-dipropylxanthine (30 nM), further increased basal ATP levels and relieved the blockade of KCl-evoked ATP release suggesting that increased A1R activation attenuates evoked ATP release in neurons ipsilateral to SNE. To determine if altered ATP release was a consequence of altered DRG metabolism we compared O2 consumption between control and neuropathic DRG. DRG ipsilateral to SNE consumed O2 at a higher rate than control or contralateral DRG. Conclusion: These data suggest that peripheral nerve entrapment increases DRG metabolism and ATP release, which in turn is modulated by increased A1R activation.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/929
metadata.dc.identifier.url: http://www.molecularpain.com/content/4/1/66
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