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Please use this identifier to cite or link to this item: http://repository.li.mahidol.ac.th/dspace/handle/123456789/942
Title: Overexpression of receptor for advanced glycation end products and high-mobility group box 1 in human dental pulp inflammation.
Authors: Salunya Tancharoen
ศรัณยา ตันเจริญ
Tassanee Tengrungsun
ทัศนีย์ เต็งรังสรรค์
Theeralaksna Suddhasthira
ธีรลักษณ์ สุทธเสถียร
Kikuchi, Kiyoshi
Vechvongvan, Nuttavun
Tokuda, Masayuki
Maruyama, Ikuro
Mahidol University. Faculty of Dentistry. Department of Pharmacology
Mahidol University. Faculty of Dentistry. Department of Advanced General Dentistry
Mahidol University. Faculty of Dentistry. Department of Pharmacology
Keywords: HMGB1 protein;Dental pulp;In vitro techniques;Immunologic;Open Access article
Issue Date: 10-Jul-2014
Citation: Tancharoen S, Tengrungsun T, Suddhasthira T, Kikuchi K, Vechvongvan N, Tokuda M, … et al. Overexpression of receptor for advanced glycation end products and high-mobility group box 1 in human dental pulp inflammation. Mediators Inflamm. 2014;2014:754069.
Abstract: High mobility group box 1 (HMGB1), a nonhistone DNA-binding protein, is released into the extracellular space and promotes inflammation. HMGB1 binds to related cell signaling transduction receptors, including receptor for advanced glycation end products (RAGE), which actively participate in vascular and inflammatory diseases. The aim of this study was to examine whether RAGE and HMGB1 are involved in the pathogenesis of pulpitis and investigate the effect of Prevotella intermedia (P. intermedia) lipopolysaccharide (LPS) on RAGE and HMGB1 expression in odontoblast-like cells (OLC-1). RAGE and HMGB1 expression levels in clinically inflamed dental pulp were higher than those in healthy dental pulp. Upregulated expression of RAGE was observed in odontoblasts, stromal pulp fibroblasts-like cells, and endothelial-like cell lining human pulpitis tissue. Strong cytoplasmic HMGB1 immunoreactivity was noted in odontoblasts, whereas nuclear HMGB1 immunoreactivity was seen in stromal pulp fibroblasts-like cells in human pulpitis tissue. LPS stimulated OLC-1 cells produced HMGB1 in a dose-dependent manner through RAGE. HMGB1 translocation towards the cytoplasm and secretion from OLC-1 in response to LPS was inhibited by TPCA-1, an inhibitor of NF-κB activation. These findings suggest that RAGE and HMGB1 play an important role in the pulpal immune response to oral bacterial infection.
URI: http://repository.li.mahidol.ac.th/dspace/handle/123456789/942
metadata.dc.identifier.url: http://www.hindawi.com/journals/mi/2014/754069/
ISSN: 0962-9351 (printed)
1466-1861 (electronic)
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