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Title: Generalized and mobilizable positive-selection cloning vectors
Authors: Skorn Mongkolsuk
Siritida Rabibhadana
Paiboon Vattanaviboon
Suvit Loprasert
Chulabhorn Research Institute
Mahidol University
Keywords: Biochemistry, Genetics and Molecular Biology
Issue Date: 27-May-1994
Citation: Gene. Vol.143, No.1 (1994), 145-146
Abstract: We have modified the positive-selection cloning vector pUN121 to expand the numbers of unique cloning sites by insertion of a multiple cloning site into the λ, ficI gene without disrupting its repressor function, resulting in plasmid pSKM10. Plasmid pSKM10 has seven restriction enzyme sites suitable for general cloning purposes. A mobilizable version (pSKM11) of pSKM10 was constructed by insertion of the IncP mob sequence which permitted mobilization of the plasmid into a wide variety of Gram - bacteria. © 1994.
ISSN: 03781119
Appears in Collections:Scopus 1991-2000

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