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Title: Flow cytometric three-color determination of CD4 T-lymphocytes on blood specimens from AIDS patients who have a large number of contaminating non-lymphocytes
Authors: K. Pattanapanyasat
V. Pengruangrojanachai
C. Thepthai
S. Suwanagool
C. Wasi
Mahidol University
Keywords: Immunology and Microbiology;Medicine
Issue Date: 1-Dec-1994
Citation: Asian Pacific Journal of Allergy and Immunology. Vol.12, No.2 (1994), 105-109
Abstract: A three-color flow cytometric determination of CD4 T-lymphocytes on whole blood specimens from AIDS patients which contain a high proportion of non-lymphocyte elements is described. Peripheral blood cells were stained by a three-color method using monoclonal antibodies conjugated respectively with fluorescein isothiocyanate (FIT)-CD3, phycoerythrin (PE)-CD4 and peridinin chlorophyll protein (PerCP)-CD45, CD45 stains all leukocytes with the highest fluorescence expression of CD45 antigen in lymphocytes. By combining light scatter with CD45 in the fluorescence 3 (FL3) channel, a light scattering window can be drawn to include almost all bright CD45 lymphocytes. This live gate of lymphocytes was then acquired and analysed simultaneously using other irrelevant two-color (FITC/PE) antibodies of CD3 and CD4 in the ATC and PE channels, respectively, This method is easy and straightforward, and gives successful analysis of CD4 T-lymphocytes in AIDS blood specimens contaminated with an unusually large number of non-lymphocytic cells.
ISSN: 0125877X
Appears in Collections:Scopus 1991-2000

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