Browsing by Author "Julien Pompon"

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    Differential susceptibility and innate immune response of aedes aegypti and aedes albopictus to the haitian strain of the mayaro virus
    (2019-10-09) Fodé Diop; Haoues Alout; Cheikh Tidiane Diagne; Michèle Bengue; Cécile Baronti; Rodolphe Hamel; Loïc Talignani; Florian Liegeois; Julien Pompon; Ronald E.Morales Vargas; Antoine Nougairède; Dorothée Missé; Animal, Santé, Territoires, Risques et Ecosystèmes (ASTRE); Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle; Aix Marseille Université; Mahidol University
    © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Mayaro (MAYV) is an emerging arthropod-borne virus belonging to the Alphavirus genus of the Togaviridae family. Although forest-dwelling Haemagogus mosquitoes have been considered as its main vector, the virus has also been detected in circulating Aedes ssp mosquitoes. Here we assess the susceptibility of Aedes aegypti and Aedes albopictus to infection with MAYV and their innate immune response at an early stage of infection. Aedes albopictus was more susceptible to infection with MAYV than Ae. aegypti. Analysis of transcript levels of twenty immunity-related genes by real-time PCR in the midgut of both mosquitoes infected with MAYV revealed increased expression of several immune genes, including CLIP-domain serine proteases, the anti-microbial peptides defensin A, E, cecropin E, and the virus inducible gene. The regulation of certain genes appeared to be Aedes species-dependent. Infection of Ae. aegypti with MAYV resulted in increased levels of myeloid differentiation2-related lipid recognition protein (ML26A) transcripts, as compared to Ae. albopictus. Increased expression levels of thio-ester-containing protein 22 (TEP22) and Niemann-Pick type C1 (NPC1) gene transcripts were observed in infected Ae. albopictus, but not Ae. aegypti. The differences in these gene expression levels during MAYV infection could explain the variation in susceptibility observed in both mosquito species.
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    New insights into the biology of the emerging tembusu virus
    (2021-01-01) Rodolphe Hamel; Thipruethai Phanitchat; Sineewanlaya Wichit; Ronald Enrique Morales Vargas; Jiraporn Jaroenpool; Cheikh Tidiane Diagne; Julien Pompon; Dorothée Missé; Faculty of Tropical Medicine, Mahidol University; Université de Montpellier; Walailak University; Mahidol University
    Reported for the first time in 1955 in Malaysia, Tembusu virus (TMUV) remained, for a long time, in the shadow of flaviviruses with human health importance such as dengue virus or Japanese encephalitis virus. However, since 2010 and the first large epidemic in duck farms in China, the threat of its emergence on a large scale in Asia or even its spillover into the human population is becoming more and more significant. This review aims to report current knowledge on TMUV from viral particle organization to the development of specific vaccines and therapeutics, with a particular focus on host–virus interactions.
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    Phylogenetic analysis revealed the co-circulation of four dengue virus serotypes in Southern Thailand
    (2019-01-01) Rodolphe Hamel; Pornapat Surasombatpattana; Sineewanlaya Wichit; Alexandra Dauvé; Celeste Donato; Julien Pompon; Dhanasekaran Vijaykrishna; Florian Liegeois; Ronald Morales Vargas; Natthanej Luplertlop; Dorothée Missé; Maladies Infectieuses et Vecteurs : Écologie, Génétique, Évolution et Contrôle; Duke-NUS Medical School Singapore; Monash University; Mahidol University; Prince of Songkla University
    © 2019 Hamel et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Dengue fever is caused by dengue viruses (DENV) from the Flavivirus genus and is the most prevalent arboviral disease. DENV exists in four immunogenically distinct and genetically-related serotypes (DENV-1 to 4), each subdivided in genotypes. Despite the endemicity of all four DENV serotypes in Thailand, no prior study has characterized the circulation of DENV in the southern provinces of the country. To determine the genetic diversity of DENV circulating in Southern Thailand in 2015 and 2016, we investigated 46 viruses from 182 patients’ sera confirmed positive for DENV by serological and Nested RT-PCR tests. Our dataset included 2 DENV-1, 20 DENV-2, 9 DENV-3 and 15 DENV-4. Phylogenetic analysis was performed on viral envelop sequences. This revealed that part of the identified genotypes from DENV-1 and DENV-4 had been predominant in Asia (genotype I for both serotypes), while genotype II for DENV-4 and the Cosmopolitan genotype DENV-2 were also circulating. Whereas DENV-3 genotype II had been predominantly detected in South East Asia during the previous decades, we found genotype III and genotype I in Southern Thailand. All DENV genotype identified in this study were closely related to contemporary strains circulating in Southeast Asian countries, emphasizing the regional circulation of DENV. These results provide new insights into the co-circulation of all four DENV serotypes in Southern Thailand, confirming the hyperendemicity of DENV in the region. These findings also suggest a new trend of dissemination for some DENV serotypes with a possible shift in genotype distribution; as recently observed in other Asian countries.
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    Zika virus infection modulates the metabolomic profile of microglial cells
    (2018-10-01) Fodé Diop; Thomas Vial; Pauline Ferraris; Sineewanlaya Wichit; Michèle Bengue; Rodolphe Hamel; Loïc Talignani; Florian Liegeois; Julien Pompon; Hans Yssel; Guillaume Marti; Dorothée Missé; Université de Toulouse; Mahidol University; CNRS Centre National de la Recherche Scientifique; Inserm
    Copyright: © 2018 Diop et al. Zika virus (ZIKV) is an emerging arbovirus of the Flaviviridae family. Although infection with ZIKV generally leads to mild disease, its recent emergence in the Americas has been associated with an increase in the development of the Guillain-Barré syndrome in adults, as well as with neurological complications, in particular congenital microcephaly, in new-borns. To date, little information is available on neuroinflammation induced by ZIKV, notably in microglial cells in the context of their metabolic activity, a series of chemical transformations that are essential for their growth, reproduction, structural maintenance and environmental responses. Therefore, in the present study we investigated the metabolomic profile of ZIKV-infected microglia. Microglial cells were exposed to ZIKV at different time points and were analyzed by a Liquid Chromatography-High Resolution mass spectrometry-based metabolomic approach. The results show that ZIKV infection in microglia leads to modulation of the expression of numerous metabolites, including lysophospholipids, particulary Lysophosphatidylcholine, and phospholipids such as Phosphatidylcholine, Phosphatidylserine, Ceramide and Sphingomyelin, and carboxylicic acids as Undecanedioic and Dodecanedioic acid. Some of these metabolites are involved in neuronal differentiation, regulation of apoptosis, virion architecture and viral replication. ZIKV infection was associated with concomitant secretion of inflammatory mediators linked with central nervous system inflammation such as IL-6, TNF-α, IL-1β, iNOS and NO. It also resulted in the upregulation of the expression of the gene encoding CX3CR1, a chemokine receptor known to regulate functional synapse plasticity and signaling between microglial cells. These findings highlight an important role for microglia and their metabolites in the process of neuroinflammation that occurs during ZIKV pathogenesis.