Browsing by Author "Marcello O. Sato"

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    Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand
    (2010-09-01) Malinee T. Anantaphruti; Munehiro Okamoto; Tippayarat Yoonuan; Surapol Saguankiat; Teera Kusolsuk; Megumi Sato; Marcello O. Sato; Yasuhito Sako; Jitra Waikagul; Akira Ito; Mahidol University; Asahikawa Medical University; Tottori University
    A community-based field survey on taeniasis and cysticercosis was performed in two villages in Thong Pha Phum District, Kanchanaburi Province, central Thailand, where 3 Taenia species, T. solium, T. saginata and T. asiatica, are sympatrically occurring. Four (0.6%) out of 667 stool samples were egg-positive for Taenia sp. by Kato-Katz technique. Three out of those four persons and other three persons who were Taenia egg-negative but having a recent (<1 year) history of discharging worms in stool were treated with niclosamide. One Taenia egg-positive woman was not treated because of severe ascites. After treatment, three persons expelled long strobilae with scolices and two persons expelled strobilae without scolex. One Taenia egg-positive person did not expel any worms post-treatment. Among 5 persons, four expelled a single worm, whereas one expelled multiple worms, may be 6 worms but not confirmed by detection of scolices. One scolex was armed with hooklets, whereas 2 others did not. Multiplex PCR of 10 expelled proglottids (including 6 estimated worms from one patient) revealed that one sample was T. solium, one T. saginata, and 8 T. asiatica. A total of 159 residents agreed to receive a serological test for cysticercosis. By ELISA using partially purified glycoprotein antigen, 9 cases, 5 and 4 from villages A and B respectively, were found to be sero-positive. The five and an additional sample on the border line from village A were evaluated using confirmative immunoblot using recombinant chimeric antigen. Among the six samples, four including the border line sample were confirmed to be cysticercosis by immunoblotting. One of the 4 persons had neurological symptoms with nodular lesions in the brain by computed tomography. These 4 confirmed or suspected cysticercosis cases were free of T. solium worms, but two of them including confirmed NCC case had a past (>1 year) history of expelling proglottids in the stool. © 2010.
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    Sympatric occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand
    (2007-01-01) Malinee T. Anantaphruti; Hiroshi Yamasaki; Minoru Nakao; Jitra Waikagul; Dorn Watthanakulpanich; Supaporn Nuamtanong; Wanna Maipanich; Somchit Pubampen; Surapol Sanguankiat; Chatree Muennoo; Kazuhiro Nakaya; Marcello O. Sato; Yasuhito Sako; Munehiro Okamoto; Akira Ito; Mahidol University; Asahikawa Medical University; Universidade Federal do Tocantins; Tottori University
    We confirmed sympatric occurrence of Taenia solium, T. saginata, and T. asiatica in western Thailand. DNA analysis of morphologically identified T. saginata, in a dual infection with T. solium, indicated it was T. asiatica. To our knowledge, this report is the first of T. asiatica and a dual Taenia infection from Thailand.