Browsing by Author "Metta Pinthong"

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    Activity and subcellular trafficking of the sodium-coupled choline transporter CHT is regulated acutely by peroxynitrite
    (2008-03-01) Metta Pinthong; Stefanie A G Black; Fabiola M. Ribeiro; Chumpol Pholpramool; Stephen S G Ferguson; Rebecca Jane Rylett; Western University; Robarts Research Institute; Mahidol University
    Excess formation of nitric oxide and superoxide by-products (peroxynitrite, reactive oxygen, and reactive nitrogen species) attenuates cholinergic transmission potentially having a role in Alzheimer disease pathogenesis. In this study, we investigated mechanisms by which acute exposure to peroxynitrite impairs function of the sodium-dependent hemicholinium-3 (HC-3)-sensitive choline transporter (CHT) that provides substrate for acetylcholine synthesis. The peroxynitrite generator 3-morpholinosydnonimine (SIN-1) acutely inhibited choline uptake in cells stably expressing FLAG-tagged rat CHT in a dose- and time-dependent manner, with an IC50 = 0.9 ± 0.14 mM and t 1/2 = 4 min. SIN-1 significantly reduced Vmax of choline uptake without altering the Km. This correlated with a SIN-1-induced decrease in cell surface CHT protein, observed as lowered levels of HC-3 binding and biotinylated CHT at the plasma membrane. It is noteworthy that short-term exposure of cells to SIN-1 accelerated the rate of internalization of CHT from the plasma membrane, but it did not alter return of CHT back to the cell surface. SIN-1 did not disrupt cell membrane integrity or cause cell death. Thus, the inhibitory effect of SIN-1 on choline uptake activity and HC-3 binding was related to enhanced internalization of CHT proteins from the plasma membrane to subcellular organelles. Copyright © 2008 The American Society for Pharmacology and Experimental Therapeutics.
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    Acute physiological responses in pregnant women during exercises in different positions
    (2019-05-04) Rungchai Chaunchaiyakul; Metta Pinthong; Amornpan Ajjimaporn; Mahidol University
    © 2018, © 2018 Taylor & Francis. Objective: This study measured acute physiological responses in pregnant women during short duration exercise in the supine (Sup), side-lying (Side), sitting (Sit), and standing (Std) positions. Methods: In a cross-sectional study, 42 healthy pregnant women were divided into 3 groups of 14 persons each: G1, G2, and G3 (first, second, and third trimester, respectively). Baseline assessments were performed following a 30-min rest in the sitting position. Subsequent measurements were then obtained while exercising, without resistance, in the Sup, Side, Sit, and Std, respectively. Physiological parameters, including heart rate (HR), minute ventilation (V E ), oxygen consumption (VO 2 ), carbon dioxide production (VCO 2 ), and oxygen pulse (VO 2 /HR), were collected using the indirect calorimetry. Results: Comparing resting values, all groups had a significantly increased (1) HR, V E , and (VCO 2 ) during the Std, (2) O 2 values during the Sit and Std, and (3) O 2 pulse values during short duration exercise in all positions except the Std, whereas only G2 and G3 had a significantly decreased HR during the Sup. Conclusion: This study points that acute physiological responses to the positional challenge similarly occur in all trimester of pregnant women. Short duration exercise in the Std positions exerts more physiologic stresses on cardiorespiratory functions than in the Sup, Side, and Sit positions.
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    Does aports bra limit metabolic profiles during constant speed running?
    (Mahidol University. Mahidol University Library and Knowledge Center, 2015) Thanachai Sahaschot; Thyon Chentanez; Rungchai Chaunchaiyakul; Metta Pinthong
    The objective of this study was to investigate whether or not sports bras distress the metabolism during jogging. Fifteen healthy female subjects participated in three exercise trials of no bra (NB), casual bra (CB), and sports bra (SB) conditions. Exercise conditions were exercise conducted on a motor-driven treadmill at 4 mph, 0% grade, and up to 80% of age-predicted maximum heart rate with randomized bra conditions. Anthropometric data and maximum oxygen consumption were determined under the CB condition. The results revealed that there were no changes in metabolic profiles of SB at rest and during exercise when compared with that of CB and NB conditions. With the exception of respiratory exchange ratio (RER), all groups exhibited similar changing patterns of rate of oxygen consumption (V̇ O2), rate of carbon dioxide production (V̇ CO2) and energy expenditure (EE), which related to the intensity of exercise, and these changes declined during the recovery period. Betweengroup comparisons showed no significant differences of metabolic variables among NB, CB, and SB. However, while NB had immediate recovery, SB recovery was delayed for 1-2 min and CB was further delayed for 3 min. In addition, this study demonstrated the critical roles of the glycolytic pathway as a main energy substrate. In conclusion, sports bras do not limit metabolic profiles at rest and during exercise but may delay some recovery processes.
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    Does sports bra limit respiratory function during constant speed exercise?
    (Mahidol University. Mahidol University Library and Knowledge Center, 2014) Kunanya Masodsai; Rungchai Chaunchaiyakul; Metta Pinthong
    The present study aimed to investigate the effect of a sports bra on respiratory function at rest and during exercise. Sixteen healthy females voluntarily and repeatedly participated in three randomized trials using no bra (NB), casual bra (CB) and sports bra (SB). They were tested using standard resting spirometer protocol. Static lung volumes included tidal volume (VT), inspiratory capacity (IC), vital capacity (VC), inspiratory reserve volume (IRV), expiratory reserve volume (ERV), respiratory rate (RR), minute ventilation (V̇ E); and dynamic lung volumes included forced expiratory volume in 1 second (FEV1.0), forced vital capacity (FVC), forced expiratory volume in 1 second and forced vital capacity ratio (%FEV1.0/FVC), maximum voluntary ventilation (MVV) and peak expiratory flow rate (PEFR) before and immediately after putting on bras. Exercise was conducted on a motor-driven treadmill at a constant speed of 4 mph continuously until the heart rate reached 60, 70, and 80% of age-predicted maximal heart rate (MHR). Only some of these lung parameters were detected during exercise. Data were compared at rest, 60, 70, and 80% MHR and every min of the 5 min recovery period. The results showed that FEV1.0, FVC, and MVV were significantly decreased (p<0.05) when compared between pre- and immediate post- SB condition. During exercise, there were no significant differences of respiratory functions between bra conditions at 60, 70, and 80%MHR. In conclusion, the sports bra exhibited reductions only for dynamic components of lung function. This effect disappeared as exercise was commenced. There was no limitation on respiratory function of either static or dynamic components during exercise. The present study indicates that this brand (Wacoal,WR1466) of commercial sports bra does not limit respiratory functions during exercise and may be appropriate for females with an active lifestyle.
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    Effect of ad libitum intake of lactose-free milk on subsequent performance of collegiate badminton athletes
    (2019-09-01) Marla Frances T. Mallari; Alisa Nana; Metta Pinthong; Saiphon Kongkum; Rungchai Chaunchaiyakul; Christian Wisdom Valleser; University of the Philippines Diliman; Mahidol University
    © 2019, Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature. The influence of dehydration on strength after a competitive match of badminton players has been previously studied; however, no research has been done on the effect of milk on performance in badminton. This study therefore aimed to investigate the effects of ad libitum intake of low-fat lactose-free milk (LFM) on subsequent performance of badminton athletes. Eleven collegiate badminton athletes (Mean ± standard deviation for Age = 19.3 ± 1.2 years; Height= 165.4 ± 9.1 cm) participated in this study. A randomized crossover study design was used (with ≥7-day washout between trials) where subjects were assigned to one of the test beverages: lactose-free skim milk (LFM), carbohydrate-electrolyte sports drink (CE) or water (CON). Performance tests were measured before and after subsequent games. Other variables measured included hydration status, perceived recovery and selected subjective perceptions. Palatability and amount ingested was highest for CE and lowest for CON (Mean ± standard deviation for CE 2215.24 ± 690.19 ml >LFM 1530.8 ± 849.9 ml >CON 1341.2 ± 424.2 ml, p = .013) but all beverages affected hydration status and selected performance variables after subsequent games in badminton. Perceived exertion and recovery were similar for the beverages as well. Thirst and gastrointestinal discomfort were however significantly greater for LFM.
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    Effects of sports drink on endurance performance in healthy Thai males after glycogen depletion
    (Mahidol University. Mahidol University Library and Knowledge Center, 2024) Sothida Nantakool; Rungchai Chaunchaiyakul; Metta Pinthong; Saiphon Kongkum
    The purpose of this study was to examine the effects of sports drink intake on endurance performance, in concomitant with cardiorespiratory, blood chemistry, and metabolic variables. Fourteen healthy males with ages ranged from 18-25 years old participated in 3 trials, with 1 week apart. Three consecutive occasions in each trial, including glycogen depletion, recovery period and endurance exercise (cycling at 70%VO2peak), were employed. During 2 hours recovery period, subjects were randomly assigned to three kinds of fluid intake including sports drink (SD, 10%carbohydrate, 0.16%electrolytes, placebo (PL, 10%carbohydrate) and plain water (WT). The time to exhaustion and work done, cardiorespiratory, blood lactate and glucose concentrations and metabolic variables were recorded. The results showed longer time to exhaustion in SD (52.93 ± 6.98 min) than PL (45.05 ± 4.47 min) and WT (37.95 ± 4.92 min), but significant difference was only observed between SD and WT (p<0.05). Significantly higher work done under SD and PL compared with WT, significantly higher ejection fraction (EF) (p<0.05) in SD than WT. Moreover, significantly lower minute ventilation under SD than PL and WT (p<0.05). There was no difference in blood lactate, blood glucose among three groups. It can be concluded that carbohydrate with electrolytes did not affect cardiorespiratory function, as well as blood chemistry profiles. However, additional electrolytes in sports drink tend to exhibit further exhaustive time.
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    Lower body immersion at different water temperatures during recovery period on thermoregulatory profiles and performance in soccer players
    (Mahidol University. Mahidol University Library and Knowledge Center, 2011) Attapol Khwankerd; Rungchai Chaunchaiyakul; Thyon Chentanez; Metta Pinthong
    The objectives of this study were to identify effect of lower body immersion, at different water temperatures (ambient, cold and warm) during 15-min recovery period on thermoregulatory profiles and the subsequent performance in soccer players. Twenty-eight soccer players were voluntarily participated in the study. To simulate soccer game conditions, the subjects were asked to complete 2 sets of 45-min intermittent sprint-cycling activities. Various recovery methods during the 15 min half-time period were randomly intervened with a control (rest without water immersion) and water immersions in ambient (27 °C), warm (39 °C) and cold (15 °C) water temperatures. After recovery intervention the subjects performed a 5-min warm up and repeated the same protocol during another 45- min intermittent sprint-cycling test. The experimental parameters were measured and recorded every five-minutes until the end of the experiment. For data analysis, ANOVA will be used for comparison among groups at specific time. Group comparison was employed using Repeated-Measured ANOVA at different time periods on the same group. The two physical performances, pre- and post-intervention, will be compared using Student t-test. Level of significance is set at p< 0.05. Rectal temperature in all groups of water immersion showed significantly lower than the control at the end of each trial (p<0.05). The rating perceived of exertion (RPE), thermal sensation, peak power, heart rate, systolic and diastolic blood pressures showed no difference from the control (p<0.05). Blood lactate in ambient and warm water immersions showed significantly lower values than in control group (p<0.05). Lower body immersion during 15 min half-time period prevented excessive rectal temperature in the end of second half but did not improve performance during second half of soccer bout.
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    Modes and five minutes duration of recovery on repeated bout of anaerobic performance in healthy young males
    (Mahidol University. Mahidol University Library and Knowledge Center, 2010) Rapeeporn Tiabtiam; Rungchai Chaunchaiyakul; Opas Sinphurmsukskul; Metta Pinthong
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    Psychomotor and cardiovascular physiological changes in elderly by training with interactive video games
    (Mahidol University. Mahidol University Library and Knowledge Center, 2014) Kewalee Wiriyaweerawat; Thyon Chentanez; Waree Widjaja; Metta Pinthong
    The aim of this study was to investigate the effects of exercise training with interactive video games on psychomotor and physiological responses in the elderly and to compare the physiological effects in older people with high blood pressure in the control group (CG) and training group with interactive video games. Thirty elderly females with an average age of 68.4 ± 6.3 years participated in this study. They were divided into two groups: training (TG) and control (CG) groups. The TG were trained by interactive video games (30 min, 3 days per week for 8 weeks) and the CG was provided with normal activity for 8 weeks. The variables in this research were ankle brachial pressure index (ABPI), resting heart rate, tapping speed testing, percentage of fat (%FAT), hip circumference ,waist circumference, hand grip strength, six minute walk test (6MWT) were all measured 3 times, at the baseline, 4th and 8th weeks, respectively. One-way repeated ANOVA and paired t-test were used to analyze the data. Significant differences were considered at the level of p < 0.05. The results showed that there were no significant changes in the hip circumference, waist circumference, grip strength, ABPI, heart rate at rest and finger tapping test of the right, left index fingers and right, left big toe. The results showed a significant decrease in body weight, percentage of fat and BMI. Six minute walk test (6MWT) increased in the TG and after training with interactive video game for 8 weeks. ABPI, muscle strength, systolic, diastolic blood pressure did not change after training for 8 weeks. In conclusion, the six minute walk test (6MWT) increased in the TG. Whereas, these were no differences in body composition and tapping speed testing. Furthermore, interactive video game exercise training did not significantly affect with their quality of life. However, interactive video game may improve the cardiovascular fitness in elderly
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    Sports bra might possibly affect cardiovascular function in active women
    (Mahidol University. Mahidol University Library and Knowledge Center, 2015) Kanchana Taothong; Thyon Chentanez; Rungchai Chaunchaiyakul; Metta Pinthong
    The aim of this study was to investigate the effect of sports bras on cardiovascular functions at rest, during and after exercise. Thirteen young habitual active females volunteered to complete 3 randomized running trials with different bra conditions of no bra (NB), casual bra (CB) and sports bra (SB) on a motor-drive treadmill at a constant speed of 6.5 km/h, 0% grade up to 60, 70 and 80% of age-predicted maximal heart rate (MHR). Continuous cardiovascular variables, including heart rate (HR), stroke volume (SV), cardiac output (CO), cardiac index (CI), end-diastolic volume (EDV), ejection fraction (EF), systemic vascular resistance (SVR), blood pressure (BP), blood perfusion (BPF), were collected at rest, during and after exercise. Results showed that all resting cardiac variables were not significantly different between groups. As exercise was started, all three conditions showed patterns of significant increase in heart rates (HR), stroke volume (SV), cardiac output (CO) and cardiac index (CI) from its corresponding resting values (p<0.05). Within group comparisons, SVR showed reduction at the beginning of exercise, EDV in SB and EF in NB and SB showed no significant differences from initial resting values. Between group comparisons during exercise showed no significant differences of most of the variables with the exception of differences of SV (p<0.05) and CO (p<0.05) between SB and CB at 60%MHR. No significant differences of other variables were detected at any intensity. During recovery period, there was an immediate decline, compared between 80%MHR and 1st min, in HR in all groups (p<0.05), SV in CB (p<0.05), CO and CI in NB (p<0.05) and CB (p<0.05) (Figure1A, 1B, 1C and 1D). There was reduction in EDV in CB (p<0.05). During recovery period, when compared to resting values, SV and EDV in all groups had no significant difference; HR, CI and CO showed significant difference throughout 5 min period (p<0.05). When compared between resting values and 1st min of recovery, EF in all groups showed significant difference (p<0.05); SVR had significant increased in all groups (p<0.05); SBP in all groups showed significant difference only in NB group (p<0.05); DBP in NB and CB showed significant difference (p<0.05); blood perfusion outside the strap in all groups showed no significant differences (p<0.05) and lastly blood perfusion inside the strap in all groups showed significant differences only in CB (p<0.05). In conclusion, both central and peripheral cardiac functions including rate and contractility, systolic and diastolic blood pressures and total vascular resistance exhibit similarly as when subjects were in no bra or casual bra. Thus, it is recommended from this study that sports bras can be used safely with no cardiovascular limitations
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    The landing error scoring system between recreational and collegiate female athletes
    (Mahidol University. Mahidol University Library and Knowledge Center, 2016) Nathasith Chutisira; Kornkit Chaijenkij; Metta Pinthong; Waree Widjaja; Weerawat Limroongreungrat
    Knee is one of the most body parts which can be injured from sport activities. The injury rate in female athletes is higher than male. Low skill athletes had higher injury rate when compared with more skilled athletes. The ACL injury is a serious injury which takes long time and high medical fee to recover. Thus, LESS was used to evaluate risks for ACL injury. The objective of this study was to find the relationship of LESS score between two groups of different skill level female athletes. Forty-four participants were recruited from female undergraduates of Mahidol University. Participants were divided into two groups due to their skill levels. Group I, Thirty participants (age 19.37 ± 1.07 yrs, height 163.40 ± 6.38 cm, weight 55.97 ± 8.37 kg, BMI 20.90 ± 2.27) and Group II, fourteen participants (age 19.29 ± 0.91 yrs, height 163.82 ± 4.82 cm, weight 58.22 ± 8.99 kg, BMI 21.66 ± 2.81). Participants did performance tests and LESS test. LESS videos were recorded by two cameras at 300 fps at frontal and sagittal plane. Pearson X2 test was used to find and independent between LESS score and skill levels. The significant level was set at p < 0.05 There was no independence between LESS score and skill levels (x2 = 8.899, df = 3, p = 0.031). In conclusion, risk for ACL injury was higher in low skill athletes regardless of physical performance.
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    Validation of the Omni Scale of perceived exertion for cycle Ergometer exercise in young female: Thai Version
    (2555) นราวุฒิ นาคคนึง; เมตตา ปิ่นทอง; Dabayebeh, Ibrahim; รุ่งชัย ชวนไชยะกูล; Robertson, Robert; Narawut Nakkanung; Metta Pinthong; Rungchai Chuanchaiyakul; มหาวิทยาลัยมหิดล. วิทยาลัยวิทยาศาสตร์และเทคโนโลยีการกีฬา
    Purpose The purpose of this study was to validate of a Thai translated version of OMNI cycle ergometer exercise scale of perceived exertion among young healthy females (N = 17) (18-25 yrs). Methods Heart rate (HR, b/min), oxygen consumption (VO2, L/min), minute ventilation (VE, L/min), respiratory rate (RR, b/min) and ratings of perceived exertion (OMNI cycle Scale; RPE) for the overall body (RPE-O), legs (RPE-L), and chest (RPE-C) were determined at the end of each of 3-min exercise stages in continuously administered exercise tests. Power output (PO) of cycling intensity started at 25 Watt (w) with 25 w incremented in every stage. Subjects performed the exercise test up to 100 w. Results Exercise responses range was for HR: 99.9-153.9 b/min; VO2: 14.20-26.58 ml/kg/min; VE: 15.1-41.7 L/min; RR: 22.3-33.2 b/min and OMNI RPE RPE-O, RPE-L, and RPE-C: 0.8-7.1. Linear regression analyses showed that RPE-O, RPE-L and RPE-C distributed as a positive linear function for all criterion measures (HR, VO2, VE, and RR) (p < 0.01). Correlation between RPE and HR (r: 0.74-0.79, p < 0.01), RPE and VO2 (r: 0.79- 0.80, p < 0.01), RPE and VE (r: 0.82-0.83, p < 0.01), and RPE and RR (r: 0.47-0.49, p < 0.01) were statistically significant. Two-way ANOVA with repeated measures showed that RPE increased at each exercise stage and RPE-L were higher (p < 0.01) than RPE-O and RPE-C. One-way ANOVA with repeated measures showed that HR, VO2, VE, and RR significantly increased with the progression of workload (p < 0.001). Conclusion The Thai translated version of the OMNI Scale of perceived exertion for cycle ergometer exercise concurrent validity is established for young adult female.