Browsing by Author "Surapol Sanguankiat"
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Publication Metadata only Abnormal Taenia Saginata tapeworms in Thailand(2011-09-01) Wanna Maipanich; Megumi Sato; Somchit Pubampen; Surapol Sanguankiat; Teera Kusolsuk; Urusa Thaenkham; Jitra Waikagul; Mahidol University; Universidade Federal do TocantinsSixty-eight residents of Ban Luang and Ban Pang Kae villages, in Nan Province, northern Thailand, visited our mobile field station in September 2006 and March 2007, seeking treatment for taeniasis. After treatment, 22 cases discharged tapeworm strobila in their fecal samples and 17 scolices were recovered. Among these, 3 were morphologically abnormal, with six suckers on the scolex. To confirm the species of these tapeworms, the mitochondrial cytochrome c oxidase subunit I (COI) gene was used as a molecular marker. The partial COI sequences (800 bp) of the abnormal tapeworms were identical to the sequences of Taenia saginata deposited in Genbank.Publication Metadata only Application of environmental DNA analysis for the detection of Opisthorchis viverrini DNA in water samples(2017-05-01) Hiroki Hashizume; Megumi Sato; Marcello Otake Sato; Sumire Ikeda; Tippayarat Yoonuan; Surapol Sanguankiat; Tiengkham Pongvongsa; Kazuhiko Moji; Toshifumi Minamoto; Kobe University; Niigata University; Dokkyo Medical University; Mahidol University; Savannakhet Provincial Health Department; Nagasaki University© 2017 Elsevier B.V. Opisthorchiasis, which can lead to cholangiocarcinoma in cases of chronic infection, is a major public health problem in Southeast Asian countries. The trematode, Opisthorchis viverrini, is the causative agent of the disease. Accurate and rapid monitoring of O. viverrini is crucial for disease prevention and containment. Therefore, in this study we sought to develop a novel species-specific real-time PCR assay for detecting O. viverrini using environmental DNA (eDNA). The diagnostic sensitivity of the newly developed real-time PCR assay was similar to that of the traditional PCR assay for 50 fecal samples collected in Lao PDR (21 and 19 samples were positive by real-time PCR and traditional PCR, respectively). The efficacy of eDNA analysis and its applicability in the field were tested using a total of 94 environmental water samples collected from 44 sites in Savannakhet, Lao PDR during May and October 2015 and February 2016. O. viverrini eDNA was detected in five samples by real-time PCR, indicating the presence of the fluke in the area and the risk of infection for individuals consuming fish from these water sources. The application of eDNA analysis would facilitate the identification of O. viverrini endemic hotspots and contribute to the ecological control of opisthorchiasis, and this strategy can be applied to other eukaryotic water pathogens.Publication Metadata only Copro-DNA diagnosis of Opisthorchis viverrini and Haplorchis taichui infection in an endemic area of LAO PDR(2010-01-01) Megumi Sato; Tiengkham Pongvongsa; Surapol Sanguankiat; Tipparayat Yoonuan; Paron Dekumyoy; Thareerat Kalambaheti; Malaythong Keomoungkhoun; Inthava Phimmayoi; Boungnong Boupha; Kazuhiko Moji; Jitra Waikagul; Mahidol University; Station of Malariology; Institute of Public Health; National Institutes for the Humanities, Research Institute for Humanity and NatureThe utility of differential copro-DNA diagnosis using modified sample preparation steps of small liver and minute intestinal fluke infections was tested. Fecal samples containing parasite eggs were washed extensively with diluted detergent solution. Parasite eggs were concentrated by sedimentation and broken by microwaving before DNA extraction. PCR targeting ITS1 and ITS2 regions were performed using primer specific for Opisthorchis viverrini, Haplorchis taichui and other related species. Of 125 fecal samples, 94 were positive for small trematode eggs by a modified cellophane thick smear method. By ITS1-PCR, 52 samples were positive for O. viverrini, 12 H. taichui and 7 mixed infection. By ITS2-PCR, 63 were positive for O. viverini, 17 H. taichui, and 19 mixed infection. The ITS-PCR assay identified a higher number of opisthorchiasis cases than those with O. viverrini expelled after treatment, but for H. taichui, ITS-PCR identified less than half of the worm expelled cases. These results showed that copro-DNA diagnosis was useful for the differential diagnosis of O. viverrini and H. taichui infection, which could not be discriminated by microscopy.Publication Metadata only Copro-molecular identification of infections with hookworm eggs in rural Lao PDR(2010-09-01) Megumi Sato; Surapol Sanguankiat; Tippayarat Yoonuan; Tiengkham Pongvongsa; Malaythong Keomoungkhoun; Inthava Phimmayoi; Boungnong Boupa; Kazuhiko Moji; Jitra Waikagul; Mahidol University; Universidade Federal do Tocantins; Malariology; National Institute of Public Health; National Institutes for the Humanities, Research Institute for Humanity and NatureThe prevalence of hookworm eggs in fecal samples, by modified cellophane thick-smear technique, was found to be 30.0% (61/203 samples) in Lahanam Village, Savannakhet Province, Lao PDR. The hookworm eggs were morphologically heterogeneous, so that identification was confirmed by copro-PCR with specific primers for hookworms and Trichostrongylus spp.; 12 samples were positive for Necator americanus, 19 for Ancylostoma spp., with one mixed infection of both. Sequencing of the Ancylostoma spp. copro-PCR products found A. duodenale, and also the animal hookworms, A. caninum and A. ceylanicum. Moreover, Trichostrongylus spp.-specific copro-PCR revealed a significant rate of infection (43/203; 21.2%). Sequencing confirmed the zoonotic species, T. colubriformis. PCR/sequencing is useful for differentiating parasite species. The positives by the Kato-Katz method, and by the combined hookworm/. Trichostrongylus specific-PCR, were comparable, although the agreement between the two methodologies was only 50%. © 2010 Royal Society of Tropical Medicine and Hygiene.Publication Metadata only Discovery of opisthorchis lobatus (Trematoda: Opisthorchiidae): A new record of small liver flukes in the greater mekong sub-region(2011-12-01) Urusa Thaenkham; Supaporn Nuamtanong; Youthanavanh Vonghachack; Tippayarat Yoonuan; Surapol Sanguankiat; Paron Dekumyoy; Bounlay Prommasack; Jun Kobayashi; Jitra Waikagul; Mahidol University; University of Health Sciences; Ministry of Health; National Center for Global Health and MedicineMetacercariae, morphologically similar to those of small liver flukes, were found to parasitize red-tailed snakehead fish, Channa limbata, collected from the city of Vientiane, Lao People's Democratic Republic. Adult worms that were recovered from experimentally-infected hamsters showed characteristics distinctly different from Opisthorchis viverrini, but closely similar to Opisthorchis lobatus, which was first reported in poultry (Anas sp.) from Pakistan. The present study aimed to redescribe O. lobatus based on the adult worms recovered from experimentally-infected hamsters. Additionally, it aimed to document the genetic relationships among O. lobatus and other opisthorchiid liver flukes using the mitochondrial cytochrome c oxidase subunit 1 (COI) gene and the internal transcribed spacer 2 (ITS2) region. DNA alignment of the O. lobatus and O. viverrini COI partial sequences (330 bp) showed 3.03% fixed differences (2.72% of amino acids changed) while the ITS2 region (350 bp) indicated a 0.86% difference for nucleotides. Species boundaries between the 2 parasites were determined by neighbor-joining analysis using the molecular sequence data. The phenogram confirmed that O. lobatus was distinctly different from O. viverrini, representing the first reported instance of O. lobatus in the Greater Mekong Sub-region (GMS) and the first record of C. limbata as the second intermediate host of a small liver fluke. Questions regarding human infection and the extent of the geographic distribution of these species should be investigated further. © 2011 American Society of Parasitologists.Item Metadata only Distribution patterns of opisthorchis viverrini and haplorchis taichui infections in two villages of sava nnakhet province, lao PDR(2013) Megumi Sato; Tiengkham Pongvongsa; Surapol Sanguankiat; สุรพล สงวนเกียรติ; Tippayarat Yoonuan; ทิพยรัตน์ อยู่นวล; Jun Kobayashi; Boungnong Boupha; Futoshi Nishimoto; Kazuhiko Moji; Marcello Otake Sato; Jitra Waikagul; จิตรา ไวคกุล; Mahidol University. Faculty of Tropical Medicine. Department of HelminthologyThe liver fluke, Opisthorchis viverrini, and the minute intestinal fluke, Haplorchis taichui, are prevalent in many Asian countries. In this study, the pattern of infection with Opisthorchis viverrini and Haplorchis tachui in Lahanam and Thakhamrien Villages (Savanakhet Province, Lao PDR) was analysed in two cross sectional investigations. Intermediate hosts fishes acquired in the study area were also analysed. Postanthelminthic treatment positivity rates for expelled worms were 170 (82.1%) for H. taichui and 65 (31.4%) for O. viverrini from a total of 207 participants; these 2 species co-existed in the study villages. Opisthorchiid egg findings initially increased with age, but decreased again from age 30 years. However, when each parasite was analysed separately, H. taichui infection reached a plateau among the age group > 20 years, and the O. viverrini infection rate was highest in the age group 21-30 years, with decreasing infection rates after age 30. Our results indicate that fish-borne trematode infections in particular are more prevalent among adults. Examination of fish intermediate hosts found O. viverrini metacercariae in only 6 species of the 35 examined, and these were mostly found during November. At this time, many farmers stay in the ricefields and obtain their food nearby, including intermediate-host fish, and may be at greater risk of O. viverrini infection. By contrast, H. taichui metacercariae were found in 3 species of fish, and only from the market, which means that anyone could consume them and become infected. If people working in ricefields limit the fish species they consume or avoid consuming raw fish around November, it may help reduce the risk of O. viverrini infection.Publication Metadata only Egg laying capacity of Haplorchis taichui (Digenea: Heterophyidae) in humans(2009-09-23) Megumi Sato; Surapol Sanguankiat; Somchit Pubampen; Teera Kusolsuk; Wanna Maipanich; Jitra Waikagui; Mahidol UniversityQuantitative fecal egg counts represented as the number of eggs per gram of feces (EPG) are generally a reliable parameter to estimate the worm burden of intestinal and hepatic parasitoses. Although Haplorchis taichui (Digenea: Heterophyidae) is one of the most common minute human intestinal flukes, little is known about the relationship between EPG and the actual worm burden in patients or the severity of the disease. In the present study, fecal samples were collected from 25 villagers in northern Thailand before and after praziquantel treatment. The EPG values of each participant were determined by the modified cellophane thick smear method, and adult worms were collected from the whole stool after the treatment. Eggs per day per worm (EPDPW) of H. taichui were estimated 82 from egg counts and expelled worms. The EPG was not well correlated with the worm burden, and a reverse correlation was observed between the EPDPW and the worm burden.Publication Metadata only Enterobiasis: A neglected infection in adults(2008-03-01) Megumi Sato; Surapol Sanguankiat; Somchit Pubampen; Teera Kusolsuk; Mahidol UniversityIn this study, adult patients were treated with praziquantel to expel intestinal flukes. Unexpectedly, dozens of adult Enterobius vermicularis worms with disfigured morphology, which had not been detected on fecal examination using Kat's modified thick-smear technique, were expelled from 6 of 33 patients.Publication Metadata only First record of paramphistomes fischoederius cobboldi and Paramphistomum epiclitum detected in bovine rumen from a local market of Savannakhet province, Lao PDR(2016-08-01) Surapol Sanguankiat; Marcello Otake Sato; Megumi Sato; Wanna Maipanich; Tippayarat Yoonuan; Tiengkham Pongvongsa; Boungnong Boupha; Yuichi Chigusa; Kazuhiko Moji; Jitra Waikagul; Mahidol University; Dokkyo Medical University; Universidade Federal do Tocantins; Niigata University; Station of Malariology; Ministry of Health; Nagasaki University© 2016, Korean Society for Parasitology and Tropical Medicine. In the present study, we report on the occurrence of paramphistomes, Fischoederius cobboldi and Paramphistomum epiclitum, in Lao PDR with the basis of molecular data. Parasite materials were collected from bovines bred in Ban Lahanam area, Savannakhet Province, Lao PDR at Lahanam public market. Morphological observations indicated 2 different species of paramphistomes. The mitochondrial gene cox1 of the specimens was successfully amplified by PCR and DNA sequencing was carried out for diagnosis of 11 specimens. Pairwise alignment of cox1 sequences were performed and confirmed F. cobboldi and P. epiclitum infecting bovines in Laos. Although there were many limiting points, as the small number of worm samples, and the restricted access of the animal host materials, we confirmed for the first time that 2 species of paramphistomes, F. cobboldi and P. epiclitum, are distributed in Lao PDR. More studies are needed to confirm the paramphistome species present in Savannakhet and its hosts to clear the natural history of these parasites of ruminants in the region and measure the impact of this parasite infection in the life and health of the local people.Publication Metadata only Genetic diversity of Taenia asiatica from Thailand and other geographical locations as revealed by cytochrome c oxidase subunit 1 sequences(2013-03-11) Malinee Thairungroj Anantaphruti; Urusa Thaenkham; Dorn Watthanakulpanich; Orawan Phuphisut; Wanna Maipanich; Tippayarat Yoonuan; Supaporn Nuamtanong; Somjit Pubampen; Surapol Sanguankiat; Mahidol UniversityTwelve 924 bp cytochrome c oxidase subunit 1 (cox1) mitochondrial DNA sequences from Taenia asiatica isolates from Thailand were aligned and compared with multiple sequence isolates from Thailand and 6 other countries from the GenBank database. The genetic divergence of T. asiatica was also compared with Taenia saginata database sequences from 6 different countries in Asia, including Thailand, and 3 countries from other continents. The results showed that there were minor genetic variations within T. asiatica species, while high intraspecies variation was found in T. saginata. There were only 2 haplotypes and 1 polymorphic site found in T. asiatica, but 8 haplotypes and 9 polymorphic sites in T. saginata. Haplotype diversity was very low, 0.067, in T. asiatica and high, 0.700, in T. saginata. The very low genetic diver- sity suggested that T. asiatica may be at a risk due to the loss of potential adaptive alleles, resulting in reduced viability and decreased responses to environmental changes, which may endanger the species. 2013, Korean Society for Parasitology and Tropical Medicine.Publication Open Access Genetic Diversity of Taenia asiatica from Thailand and Other Geographical Locations as Revealed by Cytochrome c Oxidase Subunit 1 Sequences(2013-02) Malinee Thairungroj Anantaphruti; มาลินี ไทยรุ่งโรจน์ อนันต์พฤกษ์; Urusa Thaenkham; อุรุษา แทนขำ; Dorn Watthanakulpanich; ดร วัฒนกุลพานิชย์; Orawan Phuphisut; อรวรรณ พู่พิสุทธิ์; Wanna Maipanich; วรรณา ไมพานิช; Tippayarat Yoonuan; ทิพยรัตน์ อยู่นวล; Supaporn Nuamtanong; สุภาพร น่วมทะนง; Somjit Pubampen; สมจิตร ภู่บำเพ็ญ; Surapol Sanguankiat; สุรพล สงวนเกียรติ; Malinee Thairungroj Anantaphruti; Mahidol University. Faculty of Tropical Medicine. Department of Helminthology.Twelve 924 bp cytochrome c oxidase subunit 1 (cox1) mitochondrial DNA sequences from Taenia asiatica isolates from Thailand were aligned and compared with multiple sequence isolates from Thailand and 6 other countries from the GenBank database. The genetic divergence of T. asiatica was also compared with Taenia saginata database sequences from 6 different countries in Asia, including Thailand, and 3 countries from other continents. The results showed that there were minor genetic variations within T. asiatica species, while high intraspecies variation was found in T. saginata. There were only 2 haplotypes and 1 polymorphic site found in T. asiatica, but 8 haplotypes and 9 polymorphic sites in T. saginata. Haplotype diversity was very low, 0.067, in T. asiatica and high, 0.700, in T. saginata. The very low genetic diversity suggested that T. asiatica may be at a risk due to the loss of potential adaptive alleles, resulting in reduced viability and decreased responses to environmental changes, which may endanger the species.Publication Metadata only Haplorchis taichui as a possible etiologic agent of irritable bowel syndrome-like symptoms(2010-09-01) Dorn Watthanakulpanich; Jitra Waikagul; Wanna Maipanich; Supaporn Nuamtanong; Surapol Sanguankiat; Somchit Pubampen; Rangson Praevanit; Srisuchat Mongkhonmu; Yukifumi Nawa; Mahidol UniversityThe aim of this study is to clarify the clinical features of Haplorchis taichui infection in humans in Nan Province, Thailand, and to correlate the clinical features with irritable bowel syndrome (IBS)-like symptoms. In this study area, only H. taichui, but neither other minute intestinal flukes nor small liver flukes were endemic. The degree of infection was determined by fecal egg counts and also by collecting adult worms after deworming. The signs and symptoms of individual patients together with their hematological and biochemical laboratory data were gathered to evaluate the relationship between the clinical features and the severity of infection. Special emphasis was made to elucidate the possible similarities of the clinical features of H. taichui infection and IBS-like symptoms. The results showed useful clinical information and the significant (> 50%) proportion of haplorchiasis patients complained of abdominal pain, lassitude, and flatulence, which were the important diagnostic symptoms of IBS. This study has reported a possible link between H. taichui and IBS, and H. taichui might probably play a role in the etiology of these IBS-like symptoms.Publication Metadata only Hookworm infections of schoolchildren in Southern Thailand(2002-12-01) Malinee T. Anantaphruti; Wanna Maipanich; Chatree Muennoo; Somchit Pubampen; Surapol Sanguankiat; Mahidol UniversityA study of hookworm infections of schoolchildren was conducted in Nakhon Si Thammarat Province, southern Thailand. Of the 2,940 hookworms that were recovered from the children, almost all (99.9%), were Necator americanus, only three (0.1%) were identified as Ancylostoma duodenale, and all were female worms. An estimation of the worm burden of and the worm expulsion from the schoolchildren indicated there were 17 cases of light intensity hookworm infection. Fifteen cases (88.2%) expelled worms in numbers that corresponded with the worm burden that was estimated from the number of eggs per gram of feces. Two cases (11.8%) expelled more worms than predicted. In 16 moderate intensity cases, five (31.3%) expelled worms in a quantity that corresponding with the estimated worm burden. Eleven cases (68.7%) expelled fewer worms than predicted. All cases of heavy intensity infection expelled fewer worms than predicted.Publication Metadata only Intestinal parasitic infections and environmental water contamination in a rural village of northern Lao PDR(2017-10-01) Alexis Ribas; Chloé Jollivet; Serge Morand; Boupha Thongmalayvong; Silaphet Somphavong; Chern Chiang Siew; Pei Jun Ting; Saipin Suputtamongkol; Viengsaene Saensombath; Surapol Sanguankiat; Boon Huan Tan; Phimpha Paboriboune; Kongsap Akkhavong; Kittipong Chaisiri; Universitat de Barcelona; Kasetsart University; National Institutes of Health, Bethesda; Centre d'Infectiologie Christophe Mérieux du Laos; DSO National Laboratories; Thammasat University; Luang Prabang Provincial Hospital; Mahidol University© 2017, Korean Society for Parasitology and Tropical Medicine. A field survey studying intestinal parasites in humans and microbial pathogen contamination at environment was performed in a Laotian rural village to identify potential risks for disease outbreaks. A parasitological investigation was conducted in Ban Lak Sip village, Luang Prabang, Lao PDR involving fecal samples from 305 inhabitants as well as water samples taken from 3 sites of the local stream. Water analysis indicated the presence of several enteric pathogens, i.e., Aeromonas spp., Vibrio spp., E. coli H7, E. coli O157: H7, verocytotoxin-producing E. coli (VTEC), Shigella spp., and enteric adenovirus. The level of microbial pathogens contamination was associated with human activity, with greater levels of contamination found at the downstream site compared to the site at the village and upstream, respectively. Regarding intestinal parasites, the prevalence of helminth and protozoan infections were 68.9% and 27.2%, respectively. Eight helminth taxa were identified in fecal samples, i.e., 2 tapeworm species (Taenia sp. and Hymenolepis diminuta), 1 trematode (Opisthorchis sp.), and 5 nematodes (Ascaris lumbricoides, Trichuris trichiura, Strongyloides stercoralis, trichostrongylids, and hookworms). Six species of intestinal protists were identified, i.e., Blastocystis hominis, Cyclospora spp., Endolimax nana, Entamoeba histolytica/E. dispar, Entamoeba coli, and Giardia lamblia. Questionnaires and interviews were also conducted to determine risk factors of infection. These analyses together with a prevailing infection level suggested that most of villagers were exposed to parasites in a similar degree due to limited socio-economic differences and sharing of similar practices. Limited access to effective public health facilities is also a significant contributing factor.Publication Metadata only Nematode infection among ruminants in monsoon climate (Ban-Lahanam, Lao PDR) and its role as food-borne zoonosis(2014-01-01) Marcello Otake Sato; Megumi Sato; Kittipong Chaisiri; Wanna Maipanich; Tippayarat Yoonuan; Surapol Sanguankiat; Tiengkham Pongvongsa; Boungnong Boupha; Kazuhiko Moji; Jitra Waikagul; Universidade Federal do Tocantins; Niigata University; Mahidol University; Station of Malariology; Ministry of Health; National Institutes for the Humanities, Research Institute for Humanity and NatureTrichostrongylids infection has gained significant public health importance since Trichostrongylus spp. infections have been reported in humans in Lao PDR. In this study, gastrointestinal nematodes were identified and the intensity of infections was determined in goats and cattle, which are animals greatly used for meat production in Lahanam Village, Lao PDR. The total number of goats and bovines was 23 and 29, respectively, pertaining to 32 households surveyed in the area. Feacal samples were randomly collected from 14 goats and 11 bovines. Ninety three percent (13/14) of goats and 36% (3/11) of cattle were infected, with an average of 1,728 and 86 eggs per gram of faeces (EPG), respectively. Coproculture showed Trichostrongylus spp. (goats 16%; bovines 48%), Haemonchus spp. (goats 69%; bovines 37%), Cooperia spp. (bovines 8%) and Oesophagostomum spp. (goats 15%; bovines 6%). After performing the necropsy on an adult goat, Trichuris spp. was also found. We confirmed the presence of Oesophagostomum spp., H. contortus and T. colubriformis by morphology and DNA sequencing analysis of the ITS region of rDNA. Due to interactions between humans and goats in Lahanam Village and high EPG results, the diagnosis of species and the intensity of gastrointestinal nematode infection in these animals are important public-health issues. Other ruminant parasites, such as Oesophagostomum and Haemonchus, found in caprines and bovines, are reported to be causes of zoonosis and their presence in humans should be investigated in future field surveys in this area.Publication Metadata only Nematode infection among ruminants in monsoon climate (Ban-Lahanam, Lao PDR) and its role as food-borne zoonosis(2014-03-01) Marcello O.take Otake; Megumi Sato; Kittipong Chaisiri; Wanna Maipanich; Tippayarat Yoonuan; Surapol Sanguankiat; Tiengkham Pongvongsa; Boungnong Boupha; Kazuhiko Moji; Jitra Waikagul; Universidade Federal do Tocantins; Niigata University School of Medicine; Mahidol University; Station of Malariology; Ministry of Health; National Institutes for the Humanities, Research Institute for Humanity and NatureTrichostrongylids infection has gained significant public health importance since Trichostrongylus spp. infections have been reported in humans in Lao PDR. In this study, gastrointestinal nematodes were identified and the intensity of infections was determined in goats and cattle, which are animals greatly used for meat production in Lahanam Village, Lao PDR. The total number of goats and bovines was 23 and 29, respectively, pertaining to 32 households surveyed in the area. Feacal samples were randomly collected from 14 goats and 11 bovines. Ninety three percent (13/14) of goats and 36% (3/11) of cattle were infected, with an average of 1,728 and 86 eggs per gram of faeces (EPG), respectively. Coproculture showed Trichostrongylus spp. (goats 16%; bovines 48%), Haemonchus spp. (goats 69%; bovines 37%), Cooperia spp. (bovines 8%) and Oesophagostomum spp. (goats 15%; bovines 6%). After performing the necropsy on an adult goat, Trichuris spp. was also found. We confirmed the presence of Oesophagostomum spp., H. contortus and T. colubriformis by morphology and DNA sequencing analysis of the ITS region of rDNA. Due to interactions between humans and goats in Lahanam Village and high EPG results, the diagnosis of species and the intensity of gastrointestinal nematode infection in these animals are important public-health issues. Other ruminant parasites, such as Oesophagostomum and Haemonchus, found in caprines and bovines, are reported to be causes of zoonosis and their presence in humans should be investigated in future field surveys in this area.Publication Metadata only Prevalence and clinical aspects of human Trichostrongylus colubriformis infection in Lao PDR(2013-04-01) Dorn Watthanakulpanich; Tiengkham Pongvongsa; Surapol Sanguankiat; Supaporn Nuamtanong; Wanna Maipanich; Tippayarat Yoonuan; Orawan Phuphisut; Boungnong Boupha; Kazuhiko Moji; Megumi Sato; Jitra Waikagul; Mahidol University; Station of Malariology Parasitology and Entomology; Institute of Public Health; National Institutes for the Humanities, Research Institute for Humanity and Nature; Niigata University School of MedicineThere have been few studies on human trichostrongyliasis in Southeast Asia, information on its clinical manifestations is also sparse. Trichostrongyliasis occurs predominantly in areas where poor hygiene is common especially where human/animal feces are used as a fertilizer, thereby contaminating vegetables and stream water. The intimate coexistence of domestic animals and humans explains the prevalence of Trichostrongylus infection in such areas. The goal of the current study was to determine the prevalence of trichostrongyliasis among villagers in Thakamrien village, Sonkon district, Savannakhet province, Laos, and to investigate potential relationships between clinical features, laboratory data, and severity of infection. Of 272 villagers examined, 160 (58.8%) were determined positive for helminthic infections by fecal examination, and 59 (36.9%) of these were infected with Trichostrongylus. Only 58 cases were in the inclusion criteria of the study and then underwent further assessment, including a questionnaire on personal behaviors, physical examination, and laboratory tests. Villagers in the trichostrongyliasis group were more likely than the control group to have consumed fresh vegetables, not washed their hands before meals or after using the toilet, and to have had close contact with herbivorous animals (goats and cows). Similarly, villagers in the trichostrongyliasis group were more likely than the control group to have a history of loose feces, rash, or abdominal pain; however, no obvious clinical symptoms were observed during physical examination of the trichostrongyliasis patients. The degree of infection was determined by both fecal egg counts and quantification of adult worms after deworming. Laboratory data were evaluated for any relationship with severity of infection. No significant differences were found in laboratory values between the trichostrongyliasis and control groups, with most values being within normal limits; however, both groups had high eosinophil counts. This study demonstrated that the useful clinical characteristics of trichostrongyliasis patients include history of loose feces, rashes, and abdominal pain, as well as in personal behaviors, such as the regular consumption of fresh vegetables, lack of hand washing, and close contact with cattle. © 2013 Elsevier B.V.Publication Metadata only Responses to albendazole treatment for hookworm infection in ethnic Thai and immigrant in west-central Thailand(2007-07-01) Malinee Thairungroj Anantaphruti; Supaporn Nuamtanong; Dorn Watthanakulpanich; Wanna Maipanich; Somchit Pubampen; Surapol Sanguankiat; Teera Kusolsuk; Chatree Muennoo; Jitra Waikagul; Mahidol UniversityEthnic Thai and immigrant schoolchildren and villagers of Bo-ong, a village in Pilok sub-district, Thong Pha Phum District, Kanchanaburi Province, western Thailand, were investigated for helminth infections in September 2003 and July 2004. Among the 143 schoolchildren, total cumulative hookworm prevalence in both surveys was 58.7%, with 47.6% for Thais and 63.4% for immigrants, while among the 183 villagers, it was 69.4% (Thais: 60.6%; immigrants: 75.0%). The efficacy of 400mg single-dose albendazole among different hookworm-infected racial/ethnic groups was analyzed 21 days' post-treatment. Kato-Katz and polyethylene tube cultivation methods were used for stool examination. Among the 211 hookworm-positive cases in both surveys, only 82 cases from the last survey were followed up. By Kato-Katz technique, for the schoolchildren and villagers combined, the cure rate tended to be higher among the immigrants (65.0%) than the Thais (54.6%) (p = 0.445). By Sasa's modified Harada-Mori culture technique, the cure rates also differed by racial grouping, and were higher (46.3%) among the immigrants and lower (27.8%) among the Thais (p = 0.269). However, similar egg reduction rates were found for the two racial groups, at 96.0 and 92.6%, respectively. In addition, a higher intensity of hookworm infection tolerated albendazole therapy, lower cure rates were obtained in moderate-to-heavy infections (56.3%) and higher rates for light infections (63.6%) among the total population. There were no significant differences in drug efficacy among the 2 ethnic groups of Thai and immigrants (p > 0.05) in Kanchanaburi Province, Thailand.Publication Metadata only The role of domestic dogs in the transmission of zoonotic helminthes in a rural area of Mekong river basin(2017-06-01) Marcello Otake Sato; Megumi Sato; Tippayarat Yoonuan; Tiengkham Pongvongsa; Surapol Sanguankiat; Sengchanh Kounnavong; Wanna Maipanich; Yuichi Chigusa; Kazuhiko Moji; Jitra Waikagul; Dokkyo Medical University; Niigata University; Mahidol University; Station of Malariology; Ministry of Health; Nagasaki University© 2017 W. Stefański Institute of Parasitology, PAS. Dogs have been bred since ancient times for companionship, hunting, protection, shepherding and other human activities. Some canine helminth parasites can cause significant clinical diseases in humans as Opisthorchis viverrini causing cholangiocarcinoma in Southeast Asian Countries. In this study, socio-cultural questionnaire, canine parasitological analysis, necropsy, parasite molecular confirmation and dog roaming data were evaluated in Savannakhet, Lao-PDR, a typical Mekong Basin area. Dog owners comprised 48.8% of the studied population, with 61.2% owning one dog, 25.1% 2 dogs, 8.5% 3 dogs and 1.8% owning more than 4 dogs. Data from GPS logger attached to dogs showed they walked from 1.4 to 13.3 km per day, covering an area of 3356.38m2average, with a routine of accessing water sources. Thirteen zoonotic helminth species were observed. Causative agents of visceral and cutaneous larva migrans occurred in 44.1% and 70% of the samples respectively. Spirometra erinaceieuropaei was detected in 44.1% of samples. Importantly, O. viverrini was found in 8.8% of samples. Besides the known importance of dogs in the transmission of Ancylostoma spp., Toxocara spp. and S. erinaceieuropaei, the observed roaming pattern of dogs confirmed it as an important host perpetuating O. viverrini in endemic areas; their routine access to waterbodies may spread O. viverrini eggs in a favorable environment for the fluke development, facilitating the infection of fishes, and consequently infecting humans living in the same ecosystem. Therefore, parasitic NTDs control programs in humans should be done in parallel with parasite control in animals, especially dogs, in the Mekong River basin area.Publication Metadata only Short report: Human trichostrongylus colubriformis infection in a rural village in Laos(2011-01-01) Megumi Sato; Tippayarat Yoonuan; Surapol Sanguankiat; Supaporn Nuamtanong; Tiengkham Pongvongsa; Inthava Phimmayoi; Vilayphone Phanhanan; Boungnong Boupha; Kazuhiko Moji; Jitra Waikagul; Universidade Federal do Tocantins; Mahidol University; Station of Malariology; Ministry of Health; National Institutes for the Humanities, Research Institute for Humanity and NatureIn Lahanam Village, Savannakhet Province, Laos, 125 of 253 villagers (49.4%) were found by fecal examination to harbor hookworm eggs. The eggs were heterogeneous in morphology and size, suggesting infections of mixed nematode species. To confirm the hookworm egg species, on a voluntary basis, 46 hookworm egg-positive participants were treated with albendazole, and post-treatment adult worms were collected from purged fecal samples. The common human hookworm was found in only 3 participants; 1 case of Necator americanus, and 2 cases of Ancylostoma duodenale. In contrast, adult Trichostrongylus worms were expelled from most participants (43 of 46, 93.5%). The Trichostrongylus species were confirmed by morphology and internal transcribed spacer 2 sequences; all worms were of the same species (T. colubriformis). In addition, some Trichostrongylus worms were obtained from a goat in the same village and identified as T. colubriformis. The results suggested that T. colubriformis was the main zoonotic species causing hookworm infections in the village. Copyright © 2011 by The American Society of Tropical Medicine and Hygiene.