Browsing by Author "Tanasak Changbunjong"
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Publication Metadata only Blastocystis infection and subtype distribution in humans, cattle, goats, and pigs in central and western Thailand(2018-11-01) Ruenruetai Udonsom; Rapeepan Prasertbun; Aongart Mahittikorn; Hirotake Mori; Tanasak Changbunjong; Chalit Komalamisra; Ai rada Pintong; Yaowalark Sukthana; Supaluk Popruk; Mahidol University© 2018 The Authors Blastocystis is a common intestinal pathogen of humans and a variety of animals, with various host-specific subtypes. The aim of this study was to determine the prevalence and subtype distribution of Blastocystis in humans and domestic animals, Thailand. 113 stool samples were collected from pigs, goats, and cattle in Ayutthaya Province (AP; central Thailand) and 218 stool samples were collected from pigs, dogs, cats, chickens, and humans in Kanchanaburi Province (KP; western Thailand). Blastocystis was detected by nested PCR targeting the SSU rRNA gene. Subtypes were identified by DNA sequencing, and phylogenetic analysis was conducted. The overall prevalence of Blastocystis in animals was 76.1% (86/113) and 11.88% (12/101) in AP and KP, respectively, and the prevalence in humans was 12.82% (15/117) in KP. The prevalence of Blastocystis in the AP and KP pigs were 87.88% (29/33) and 20.37% (11/54), respectively. Blastocystis ST5 was the most abundant in pigs in both areas while Blastocystis ST10 and ST12 were most frequently found in cattle and goats. In addition, low percentage of Blastocystis ST1 and Blastocystis ST14 were found in pigs and goats, respectively. In this study, Blastocystis ST3, followed by ST2 and ST1 were predominantly found in humans. In conclusion, pigs may be a natural host of Blastocystis and this ST may be the pig-adapted ST in the studied areas, in this study.Publication Metadata only Blastocystis subtype 5: Predominant subtype on pig farms, Thailand(2018-12-01) Ai rada Pintong; Suparat Sunyanusin; Rapeepan Prasertbun; Aongart Mahittikorn; Hirotake Mori; Tanasak Changbunjong; Chalit Komalamisra; Yaowalark Sukthana; Supaluk Popruk; Mahidol University© 2018 The Authors Blastocystis is a unicellular protist most commonly detected in humans and a variety of animals. The predominant mode of its transmission is the fecal–oral route, but its zoonotic potential is not completely understood. The objective of this study was to determine the presence and genetic diversity of Blastocystis on pig farms in Nakhon Pathom Province, Central Thailand. A total of 154 human and 90 pig stool samples were collected and analyzed. Nested PCR detected Blastocystis in 35.55% of the pig samples and 6.49% of the human samples. Subtyping based on regions of the small-subunit ribosomal RNA (SSU rRNA) gene identified three Blastocystis subtypes in pigs and humans: ST1, ST3, and ST5. Blastocystis ST5 was the predominant subtype, followed by ST1 and then ST3. All the sequences from the Blastocystis-positive samples from both pigs and humans were closely related. This study reveals a possibility of low host specificity of Blastocystis STs (ST1, ST3 and ST5) on pig farms in Thailand. We tentatively suggest that close contact with or exposure to pig stools may be a significant source of Blastocystis detected in pig handlers. Further studies are required to confirm the zoonotic transmission of this organism in Thailand, because pigs may play an important role in the transmission of Blastocystis.Publication Metadata only Distribution and abundance of Stomoxyini flies (Diptera: Muscidae) in Thailand(2012-11-01) Tanasak Changbunjong; Thekhawet Weluwanarak; Parntep Ratanakorn; Pattarapon Maneeon; Manoch Ganpanakngan; Chamnarn Apiwathnasorn; Sungsit Sungvornyothin; Patchara Sriwichai; Suchada Sumruayphol; Jiraporn Ruangsittichai; Mahidol University; Ministry of Natural Resources and EnvironmentStomoxyini flies (Diptera: Muscidae) include species of parasitic flies of medical and veterinary importance. The adult flies feed on the blood of mammals and may transmit several parasites and pathogens. We conducted an entomological survey of Stomoxyini flies from different sites in Thailand. Stomoxyini flies were collected at four major types of sites: zoos, livestock farms, wildlife conservation areas and a national park using vavoua traps between November 2010 and April 2011. A total of 3,314 Stomoxyini flies belonging to the genera Stomoxys, Haematobosca, Haematostoma and Haematobia were collected. Eight species were identified: S. calcitrans (46.6%), S. uruma (26.8%), S. pulla (4.3%), S. indicus (0.7%), S. sitiens (0.1%), H. sanguinolenta (11.2 %), H. austeni (0.5%) and H. irritans exigua (9.8%). The diversity of Stomoxyini flies in the livestock farms was higher than the other sites. Altitude correlated with the number of flies. This study provides information that may be useful for Stomoxyini flies control.Publication Open Access The distribution of Kitti's hog-nosed Bat (Craseonycteris thonglongyai) in Kanchanaburi Province(2013) Poonyapat Sedwisai; Tanasak Changbunjong; Tatiyanuch Chamsai; Siriporn Tangsudjai; Sukuman Rittem; Thekhawet Weluwanarak; Sineenard Jiemtaweeboon; Kanokporn Triwitayakorn; Pitchapat Hanprab; Surachit Waengsothorn; Ruangrat Buddhirongawatr; Kridsada Chaichoun; Mahidol University. Faculty of Veterinary Science; Mahidol University. Institute of Molecular BiosciencesThe distribution of Kitti's Hog-nosed Bat (Craseonycteris thonglongyai) was conducted in Saiyok District, Kanchanaburi Province using harp traps between April 2010 and April 2013. This species was found in 11 out of 24 caves; 9 in military area and 2 in conservation area. A total of 672 bats were captured in this survey. The length of the bat forearms were 23.0-26.3 mm and adults weighed between 1.22 and 5.94 g. Results of this study provide information for conservation of this bat in the area.Publication Metadata only DNA barcode identification and molecular detection of bluetongue virus in Culicoides biting midges (Diptera: Ceratopogonidae) from western Thailand(2021-12-01) Yuki Fujisawa; Thanyaporn Homat; Arunrat Thepparat; Tanasak Changbunjong; Kripitch Sutummaporn; Sudsaijai Kornmatitsuk; Bunlue Kornmatitsuk; Ramkhamhaeng University; Mahidol UniversityBiting midges of the genus Culicoides Latreille are biological vectors of bluetongue virus (BTV), a member of family Reoviridae, genus Orbivirus. About 30 species of Culicoides have been identified as competent BTV vectors worldwide. Even though high seroprevalence of BTV has been reported among livestock ruminants from western Thailand, the Culicoides species which contribute to BTV transmission remain unclear. In the present study, Culicoides were collected from eight sampling sites, located in two BTV prevalent provinces in western Thailand. Adult Culicoides were identified using wing morphology and cytochrome c oxidase subunit I (COI) mtDNA molecular marker. A total of 9,677 Culicoides specimens belonging to 7 subgenera, 3 species groups, and 23 species were identified. After comparing sequencing results with available data from GenBank, COI sequences of five species were reported for the first time from Thailand. The most abundant potential BTV vector species collected were C. peregrinus, followed by C. orientalis, C. imicola, C. oxystoma, and C. fulvus. Out of 72 Culicoides pools, 9 pools (4 from C. orientalis, 2 from C. imicola, 2 from C. oxystoma, and 1 from C. fulvus) were positive by BTV RT-PCR analyses. These results are new to Culicoides BTV vector knowledge in Thailand and will contribute to further BTV studies in this particular region.Item Metadata only DNA barcoding of the parasitic flies, stomoxys flies (diptera: muscidae), from natural sources in Thailand(2013) Jiraporn Ruangsittichai; จิราภรณ์ เรืองสิทธิชัย; Tanasak Changbunjong; Thekhawet Weluwanarak; Chamnarn Apiwathnasorn; ชำนาญ อภิวัฒนศร; Sungsit Sungvornyothin; สังสิทธิ์ สังวรโยธิน; Patchara Sriwichai; พัชรา ศรีวิชัย; Suchada Sumruayphol; สุชาดา สำรวยผล; Mahidol University. Faculty of Tropical Medicine. Department of Medical EntomologyThe Stomoxys flies are known as parasitic flies allecting livestock and wildlife. They have determined as high impact in economic lost especially dairy farm. Adult flies feed on blood causing blood loss and reducing milk product. They are major irritant pests of livestock and transmit many pathogens to cattle. The Stomoxys flies were collected from many parts of Thailand. Species were firstly identified based on morphological characteristics. There are some Stomoxys species, Stomoxys indicus, Stomoxys pulla and Stomoxys uruma, which are poorly identified by morphology. DNA barcoding has been developed to solve this problem. A 657 bp of COI barcodes from five species, Stomoxys calcitrans Stomoxys indicus, Stomoxys pulla, Stomoxys sitiens and Stomoxys uruma, could be amplified. Their DNA sequences were made phylogenetic analysis and calculated sequence divergence by MEGA 5.1 program. The average of interspecific sequence divergence ranged 5.2- 12.0%. The neighbor-joining (NJ) analysis among five species of the Stomoxyini flies was analysed using K2P parameter. All of Stomoxys flies were clearly separated into each species. The DNA barcoding can be used to confirm the poorly discriminated species and is applied for managing control programs of parasitic flies.Publication Metadata only Ectoparasitic fauna of birds, and volant and non-volant small mammals captured at Srinakarin Dam, Kanchanaburi, Thailand(2010-05-01) Tanasak Changbunjong; Charoonluk Jirapattharasate; Ruangrat Buddhirongawatr; Kacha Chewajon; Pavinee Charoenyongyoo; Sarin Suwanapakdee; Surachit Waengsothorn; Kanokporn Triwitayakorn; Kridsada Chaichoun; Parntep Ratanakorn; Mahidol University; Thailand Institute of Scientific and Technological Research (TISTR)The investigation of ectoparasitic fauna on birds and volant and nonvolant small mammals at srinakarin dam Kanchanaburi province Thailand was carried out under a national biodiversity and disease surveillance program for four consecutive months: January February May and june 2009. a total of 122 animals Comprised of 15 species of birds, 9 species of volant small mammals and 8 species of non-volant small mammals were examined for ectoparasite infestation. of these animals 1 genus of hard ticks (ixodidae), 2 species of mesostigmatid mites (laelapidae), 4 genera in three families of astigmatid mites (proctophyllodidae, Pteronyssidae and trouessartiidae),4 species in three families of lice (philopteridae Polyplacidae and trichodectidae) and 2 families of batflies (nycteribiidae and streblidae) were collected. This is the first survey conducted to determine ectoparasites infesting birds and small mammals living in the reserved forest of srinakarin dam Thailand. a lower infestation rate of ectoparasites was observed in mammals, Ranging from 3.5% to 10.3% than birds With infestation rates between 7.3% and 34.2%. no major potential health risks to people who lived in this area were found.Publication Metadata only Effect of crude extract of Solanum xanthocarpum against snails and mosquito larvae(2010-03-01) Tanasak Changbunjong; Waranya Wongwit; Somjai Leemingsawat; Yuwadee Tongtokit; Vanida Deesin; Mahidol UniversityThe ethanolic crude extract from Solanum xanthocarpum was investigated for its molluscicidal activity against Biomphalaria glabrata, the snail vector of Schistosoma mansoni, and Indoplanorbis exustus, the snail vector of intestinal echinostomiasis and Schistosoma spindale, together with the larvicidal activity against the larvae of Aedes aegypti, mosquito vector of dengue hemorrhagic fever and Culex quinquefasciatus, the mosquito vector of urban bancroftian filariasis. The bioassays were carried out following the methods recommended by the World Health Organization. For molluscicidal activity, the LC50 against Bi. glabrata and I. exustus were reported at 163.85 and 198.00 mg/l while the LC90 were 219.33 and 236.80 mg/l, respectively. Regarding mosquito larvicidal activity, the LC50 against the larvae of Ae. aegypti and Cx. quinquefasciatus were 788.10 and 573.20 mg/l, while the LC 90 were 1,288.91 and 1,066.93 mg/l, respectively. These results suggest a preparation of ingredients from this plant may be used as a biological larvicide for these vectors in the field.Publication Metadata only Erratum to Ectoparasitic fauna of birds, and volant and non-volant small mammals captured at srinakarin dam, Kanchanaburi, Thailand (The Southeast Asian Journal of Tropical Medicine and Public Health, (2010), 41, 3, (526-535))(2010-09-01) Tanasak Changbunjong; Charoonluk Jirapattharasate; Ruangrat Buddhirongawatr; Kacha Chewajon; Pavinee Charoenyongyoo; Sarin Suwanapakdee; Surachit Waengsothorn; Kanokporn Triwitayakorn; Kridsada Chaichoun; Parntep Ratanakorn; Mahidol University; Thailand Institute of Scientific and Technological Research (TISTR)Publication Metadata only First report of Ctenocephalides felis felis on the Asiatic Golden Cat, Catopuma temminckii in Thailand(2011-08-08) Tanasak Changbunjong; Nareerat Sangkachai; Siriporn Tangsudjai; Mahidol UniversityThe cat flea, Ctenocephalides felis felis (Siphonaptera: Pulicidae) has been reported to parasitize many species of wild and domestic animals and could serve as a vector of zoonotic pathogens. This is the first report of the presence of cat fleas on the Asiatic Golden Cat, Catopuma temminckii (Carnivora: Felidae), quarantined in Khao Pratabchang Wildlife and Breeding Center, Ratchaburi Province, Thailand. The findings of this report may be helpful in evaluating the potential risks associated with increased contact between wild and domestic animals and humans in this region.Publication Open Access Heavy Infestation of the Sticktight Flea (Echidnophaga gallinacea) in Dog(2009) Thekhawet Weluwanarak; Siriporn Tangsudjai; Seni Ngamloephochit; Tanasak Changbunjong; Mahidol University. Faculty of Veterinary SciencePublication Metadata only Identification of ehrlichia spp in canines in Thailand(2012-11-19) Charoonluk Jirapattharasate; Jarin Chatsiriwech; Parut Suksai; Tanasak Changbunjong; Thanakorn Rawangchue; Walasinee Moonarmart; Sivapong Sungpradit; Mahidol UniversityCanine ehrlichiosis is an endemic parasitic disease widely found in Thailand. The causative microorganism is tick-borne Ehrlichia spp, an obligate intracellular rickettsia residing in leukocytes. Ehrlichia spp in morulae-positive canine blood samples were identified using polymerase chain reaction amplification and direct sequencing of Ehrlichia spp. 16S rDNA 396 bp fragment and 36 of 59 were positive for E. canis. E. chaffeensis and E. ewingii were not detected. Sequencing alignment and phylogenetic analysis showed that 16S rDNA sequences of E. canis strains are 99.1-100% identical among E. canis strains from different countries worldwide. Further studies are required in order to determine new target sequence for genotyping of E. canis strains in the dog population in Thailand.Item Metadata only Laboratory investigation of the molluscicidal and mosquito larvicidal activities of yellow-berried nightshade, Solanum xanthocarpum schrad. & wendl.(Mahidol University. Mahidol University Library and Knowledge Center, 2007) Tanasak Changbunjong; Waranya WongwitPublication Metadata only Landmark and outline-based geometric morphometrics analysis of three Stomoxys flies (Diptera: Muscidae)(2016-01-01) Tanasak Changbunjong; Suchada Sumruayphol; Thekhawet Weluwanarak; Jiraporn Ruangsittichai; Jean Pierre Dujardin; Mahidol University; IRD Centre de Montpellier© Institute of Parasitology, Biology Centre CAS. Adult flies of the genus Stomoxys Geoffroy, 1762 (Diptera: Muscidae), especially S. pullus Austen, 1909, S. uruma Shinonaga et Kano, 1966 and S. indicus Picard, 1908, are morphologically similar and sometimes difficult to distinguish when using external morphological characteristics. These species may act as vectors and/or potential vectors of many pathogens (virus, bacteria and protozoa). Their correct identification is important to target the vectors involved in the transmission of the pathogens and also helps in the fly control program.The aim of the present study was to distinguish three species which are difficult to separate using traditional diagnostic characters for species of Stomoxys such as colour patterns and body proportions. Modern morphometrics, both landmark and outline-based, was used to access wing geometry of S. pullus, S. uruma and S. indicus. A total of 198 and 190 wing pictures were analysed for landmark- and outline-based approaches, respectively. Wing shape was able to separate species and sexes of the three Stomoxys flies with highly significant difference of Mahalanobis distances. The cross-validated classification scores ranged from 76% to 100% for landmark and 77% to 96% for outline-based morphometrics. The geometry of wing features appears to be a very useful, low-cost tool to distinguish among the vectors S. pullus, S. uruma and S. indicus.Publication Metadata only Landmark data to distinguish and identify morphologically close tabanus spp. (diptera: Tabanidae)(2021-11-01) Tanasak Changbunjong; Nutnicha Prakaikowit; Photchanun Maneephan; Tipparat Kaewwiset; Thekhawet Weluwanarak; Tanawat Chaiphongpachara; Jean Pierre Dujardin; Suan Sunandha Rajabhat University; IRD Centre de Montpellier; Mahidol UniversityTabanus spp., also known as horse flies (Diptera: Tabanidae), are important vectors of several animal pathogens. Adult females of Tabanus megalops and Tabanus striatus, which are members of the T. striatus complex, are morphologically similar and hence difficult to distinguish using morphological characteristics. In addition, molecular identification by DNA barcoding is also unable to distinguish these species. These two species can occur sympatrically with Tabanus rubidus, which is morphologically similar to T. megalops and T. striatus. Wing geometric morphometrics has been widely used in various insects to distinguish morphologically similar species. This study explored the effectiveness of landmark-based geometrics at distinguishing and identifying T. megalops, T. rubidus, and T. striatus in Thailand. Specimens were collected from different geographical regions of Thailand, and only unambiguously identified specimens were used for geometric morphometric analyses. Left wings of females of T. megalops (n = 160), T. rubidus (n = 165), and T. striatus (n = 85) were photographed, and 22 wing landmarks were used for the analysis. Wing shape was able to distinguish among species with high accuracy scores, ranging from 94.38% to 99.39%. We showed that morphologically very close species of Tabanus can be reliably distinguished by the geometry of their wing venation, and we showed how our experimental material could be used as a reference to tentatively identify new field collected specimens.Publication Metadata only Molecular characterization of Hepatozoon felis in Rhipicephalus sanguineus ticks infested on captive lions (Panthera leo)(2017-09-01) Benjaporn Bhusri; Ladawan Sariya; Chalisa Mongkolphan; Parut Suksai; Supakarn Kaewchot; Tanasak Changbunjong; Mahidol University; Department of Natural Parks© 2017, Indian Society for Parasitology. Hepatozoon spp. are protozoan parasites that infect a wide range of domestic and wild animals. The infection occurs by ingestion of an infected tick. This study was carried out to detect and characterize Hepatozoon spp. in ticks collected from captive lions (Panthera leo) in Thailand based on the partial 18S rRNA gene sequence. A total of 30 ticks were collected and identified as Rhipicephalus sanguineus. The collected ticks were separated into 10 tick pools by sex and life stages. Of the 10 tick pools examined, only one (10%) was found to be infected with the Hepatozoon species. Sequencing and phylogenetic analysis showed a clustering of the partial 18S rRNA gene sequence like that of H. felis from the GenBank database. This is the first report of H. felis in R. sanguineus ticks collected from captive lions in Thailand. Our results indicated that R. sanguineus may be a possible vector of feline Hepatozoon in Thailand.Publication Metadata only Molecular detection and genetic diversity of bovine Babesia spp., Theileria orientalis, and Anaplasma marginale in beef cattle in Thailand(2017-02-01) Charoonluk Jirapattharasate; Paul Franck Adjou Moumouni; Shinuo Cao; Aiko Iguchi; Mingming Liu; Guanbo Wang; Mo Zhou; Patrick Vudriko; Artemis Efstratiou; Tanasak Changbunjong; Sivapong Sungpradit; Parntep Ratanakorn; Walasinee Moonarmart; Poonyapat Sedwisai; Thekhawet Weluwanarak; Witsanu Wongsawang; Hiroshi Suzuki; Xuenan Xuan; Obihiro University of Agriculture and Veterinary Medicine; Mahidol University© 2016, Springer-Verlag Berlin Heidelberg. Babesia spp., Theileria orientalis, and Anaplasma marginale are significant tick-borne pathogens that affect the health and productivity of cattle in tropical and subtropical areas. In this study, we used PCR to detect the presence of Babesia bovis, Babesia bigemina, and T. orientalis in 279 beef cattle from Western Thailand and A. marginale in 608 beef cattle from the north, northeastern, and western regions. The PCRs were performed using species-specific primers based on the B. bovis spherical body protein 2 (BboSBP2), B. bigemina rhoptry-associated protein 1a (BbiRAP-1a), T. orientalis major piroplasm surface protein (ToMPSP), and A. marginale major surface protein 4 (AmMSP4) genes. To determine the genetic diversity of the above parasites, amplicons of B. bovis and B. bigemina ITS1-5.8s rRNA gene-ITS2 regions (B. bovis ITS, B. bigemina ITS), ToMPSP, and AmMSP4 genes were sequenced for phylogenetic analysis. PCR results revealed that the prevalence of B. bovis, B. bigemina, T. orientalis, and A. marginale in the Western region was 11.1, 12.5, 7.8, and 39.1 %, respectively. Coinfections of two or three parasites were observed in 17.9 % of the animals sampled. The study revealed that the prevalence of A. marginale in the western region was higher than in the north and northeastern regions (7 %). Sequence analysis showed the BboSBP2 gene to be more conserved than B. bovis ITS in the different isolates and, similarly, the BbiRAP-1a was more conserved than B. bigemina ITS. In the phylogenetic analysis, T. orientalis MPSP sequences were classified into types 3, 5, and 7 as previously reported. A. marginale MSP4 gene sequences shared high identity and similarity with each other and clustered with isolates from other countries. This study provides information on the prevalence and genetic diversity of tick-borne pathogens in beef cattle and highlights the need for effective strategies to control these pathogens in Thailand.Publication Metadata only Molecular epidemiology of bovine Babesia spp. and Theileria orientalis parasites in beef cattle from northern and northeastern Thailand(2016-02-01) Charoonluk Jirapattharasate; Paul Franck Adjou Moumouni; Shinuo Cao; Aiko Iguchi; Mingming Liu; Guanbo Wang; Mo Zhou; Patrick Vudriko; Tanasak Changbunjong; Sivapong Sungpradit; Parntep Ratanakorn; Walasinee Moonarmart; Poonyapat Sedwisai; Thekhawet Weluwanarak; Witsanu Wongsawang; Hiroshi Suzuki; Xuenan Xuan; Obihiro University of Agriculture and Veterinary Medicine; Mahidol University© 2015 Elsevier Ireland Ltd. Beef cattle production represents the largest cattle population in Thailand. Their productivity is constrained by tick-borne diseases such as babesiosis and theileriosis. In this study, we determined the prevalence of Babesia bigemina, Babesia bovis and Theileria orientalis using polymerase chain reaction (PCR). The genetic markers that were used for detection of the above parasites were sequenced to determine identities and similarity for Babesia spp. and genetic diversity of T. orientalis. Furthermore the risk factors for the occurrence of the above protozoan parasites in beef cattle from northern and northeastern parts of Thailand were assessed. A total of 329 blood samples were collected from beef cattle in 6 provinces. The study revealed that T. orientalis was the most prevalent (30.1%) parasite in beef cattle followed by B. bigemina (13.1%) and B. bovis (5.5%). Overall, 78.7% of the cattle screened were infected with at least one of the above parasites. Co-infection with Babesia spp. and T. orientalis was 30.1%. B. bigemina and T. orientalis were the most prevalent (15.1%) co-infection although triple infection with the three parasites was observed in 3.0% of the samples. Sequencing analysis revealed that B. bigemina RAP1 gene and B. bovis SBP2 gene were conserved among the parasites from different cattle samples. Phylogenetic analysis showed that the T. orientalis MPSP gene from parasites isolated from cattle in north and northeast Thailand was classified into types 5 and 7 as reported previously. Lack of tick control program was the universal risk factor of the occurrence of Babesia spp. and T. orientalis infection in beef cattle in northern and northeastern Thailand. We therefore recommend training of farmers on appropriate tick control strategies and further research on potential vectors for T. orientalis and elucidate the effect of co-infection with Babesia spp. on the pathogenicity of T. orientalis infection on beef in northern and northeastern Thailand.Publication Metadata only Molecular identification and geometric morphometric analysis of haematobosca aberrans (Diptera: Muscidae)(2020-07-01) Tanasak Changbunjong; Jiraporn Ruangsittichai; Gerard Duvallet; Adrian C. Pont; University of Oxford; Mahidol University; Universite Paul-Valery Montpellier III© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The genus Haematobosca Bezzi, 1907 (Diptera: Muscidae) contains haematophagous flies of veterinary importance. A new fly species of this genus was recognised from northern Thailand based on morphological characters and described as Haematobosca aberrans Pont, Duvallet & Changbunjong, 2020. In the present study, the mitochondrial cytochrome c oxidase I (COI) gene was used to confirm the morphological identification of H. aberrans. In addition, landmark-based geometric morphometrics was used to determine sexual dimorphism. The molecular analysis was conducted with 10 COI sequences. The results showed that all sequences were 100% identical. The sequence was not highly similar to reference sequences from GenBank and did not match any identified species from Barcode of Life Data Systems (BOLD). Phylogenetic analysis clearly differentiated this species from other species within the subfamily Stomoxyinae. For geometric morphometric analysis, a total of 16 wing pictures were analysed using the landmark-based approach. The results showed significant differences in wing shape between males and females, with a cross-validated classification score of 100%. The allometric analysis showed that wing shape has no correlation with size. Therefore, the COI gene is effective in species identification of H. aberrans, and geometric morphometrics is also effective in determining sexual dimorphism.Publication Metadata only Mosquito distribution and West Nile virus infection in zoos and in important sites of migratory and resident birds, Thailand(2012-08-01) Tanasak Changbunjong; Thekhawet Weluwanarak; Namaoy Toawan; Parut Suksai; Poonyapat Sedwisai; Tatiyanuch Chamsai; Charoonluk Jirapattharasate; Sivapong Sungpradit; Yudthana Samung; Parntep Ratanakorn; Mahidol UniversityObjective: To investigate the distribution of mosquito species in the zoos and in important sites of migratory and resident birds and evaluate West Nile virus (WNV) infection in mosquito species. Methods: Mosquitoes distribution investigation was carried out bimonthly from January 2009 to December 2010 in five areas of birds, Thailand by using Centers for Disease Control, light traps, and gravid traps. Mosquitoes were identified, pooled into groups of up to 50 mosquitoes by species, places and time of collection and tested for WNV infection by viral isolation and reverse transcriptase polymerase chain reaction. Results: A total of 66 597 mosquitoes comprising 26 species in 8 genera were collected. The five most abundant mosquito species collected were Culex tritaeniorhynchus (79.3%), Culex vishnui (8.2%), Culex sitiens (6%), Culex quinquefasciatus (3.3%) and Anopheles peditaeniatus (1.1%). All 1 736 mosquito pools were negative for viral isolation and reverse transcriptase polymerase chain reaction. Conclusion: This study provides new information on number of mosquito species present and their relative abundance. Although our s tudy found no evidence of WNV in the avifaunal sources of Thailand, mosquito active surveillance should be continuously conducted. The cooperation between related organizations is needed for early detection of WNV disease and development of effective veterinary and public health policies in this region. © 2012 Asian Pacific Tropical Medicine Press.