Browsing by Author "Universidade Federal do Tocantins"

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    Abnormal Taenia Saginata tapeworms in Thailand
    (2011-09-01) Wanna Maipanich; Megumi Sato; Somchit Pubampen; Surapol Sanguankiat; Teera Kusolsuk; Urusa Thaenkham; Jitra Waikagul; Mahidol University; Universidade Federal do Tocantins
    Sixty-eight residents of Ban Luang and Ban Pang Kae villages, in Nan Province, northern Thailand, visited our mobile field station in September 2006 and March 2007, seeking treatment for taeniasis. After treatment, 22 cases discharged tapeworm strobila in their fecal samples and 17 scolices were recovered. Among these, 3 were morphologically abnormal, with six suckers on the scolex. To confirm the species of these tapeworms, the mitochondrial cytochrome c oxidase subunit I (COI) gene was used as a molecular marker. The partial COI sequences (800 bp) of the abnormal tapeworms were identical to the sequences of Taenia saginata deposited in Genbank.
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    Copro-molecular identification of infections with hookworm eggs in rural Lao PDR
    (2010-09-01) Megumi Sato; Surapol Sanguankiat; Tippayarat Yoonuan; Tiengkham Pongvongsa; Malaythong Keomoungkhoun; Inthava Phimmayoi; Boungnong Boupa; Kazuhiko Moji; Jitra Waikagul; Mahidol University; Universidade Federal do Tocantins; Malariology; National Institute of Public Health; National Institutes for the Humanities, Research Institute for Humanity and Nature
    The prevalence of hookworm eggs in fecal samples, by modified cellophane thick-smear technique, was found to be 30.0% (61/203 samples) in Lahanam Village, Savannakhet Province, Lao PDR. The hookworm eggs were morphologically heterogeneous, so that identification was confirmed by copro-PCR with specific primers for hookworms and Trichostrongylus spp.; 12 samples were positive for Necator americanus, 19 for Ancylostoma spp., with one mixed infection of both. Sequencing of the Ancylostoma spp. copro-PCR products found A. duodenale, and also the animal hookworms, A. caninum and A. ceylanicum. Moreover, Trichostrongylus spp.-specific copro-PCR revealed a significant rate of infection (43/203; 21.2%). Sequencing confirmed the zoonotic species, T. colubriformis. PCR/sequencing is useful for differentiating parasite species. The positives by the Kato-Katz method, and by the combined hookworm/. Trichostrongylus specific-PCR, were comparable, although the agreement between the two methodologies was only 50%. © 2010 Royal Society of Tropical Medicine and Hygiene.
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    First record of paramphistomes fischoederius cobboldi and Paramphistomum epiclitum detected in bovine rumen from a local market of Savannakhet province, Lao PDR
    (2016-08-01) Surapol Sanguankiat; Marcello Otake Sato; Megumi Sato; Wanna Maipanich; Tippayarat Yoonuan; Tiengkham Pongvongsa; Boungnong Boupha; Yuichi Chigusa; Kazuhiko Moji; Jitra Waikagul; Mahidol University; Dokkyo Medical University; Universidade Federal do Tocantins; Niigata University; Station of Malariology; Ministry of Health; Nagasaki University
    © 2016, Korean Society for Parasitology and Tropical Medicine. In the present study, we report on the occurrence of paramphistomes, Fischoederius cobboldi and Paramphistomum epiclitum, in Lao PDR with the basis of molecular data. Parasite materials were collected from bovines bred in Ban Lahanam area, Savannakhet Province, Lao PDR at Lahanam public market. Morphological observations indicated 2 different species of paramphistomes. The mitochondrial gene cox1 of the specimens was successfully amplified by PCR and DNA sequencing was carried out for diagnosis of 11 specimens. Pairwise alignment of cox1 sequences were performed and confirmed F. cobboldi and P. epiclitum infecting bovines in Laos. Although there were many limiting points, as the small number of worm samples, and the restricted access of the animal host materials, we confirmed for the first time that 2 species of paramphistomes, F. cobboldi and P. epiclitum, are distributed in Lao PDR. More studies are needed to confirm the paramphistome species present in Savannakhet and its hosts to clear the natural history of these parasites of ruminants in the region and measure the impact of this parasite infection in the life and health of the local people.
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    Foodborne trematodes
    (2015-06-04) J. Waikagul; M. Sato; M. O. Sato; Mahidol University; Niigata University; Universidade Federal do Tocantins
    © 2015 Elsevier Ltd All rights reserved. Trematodes are reviewed in groups, based on the source of their infective stage, either vegetables or animals (crustaceans, fish, snails, insects, amphibians, and reptiles). The biology, epidemiology, pathogenesis, and symptoms of infection of species reported in humans are discussed. The chapter concludes with a summary of information on diagnosis, treatment, prevention, and control of these trematodes.
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    Nematode infection among ruminants in monsoon climate (Ban-Lahanam, Lao PDR) and its role as food-borne zoonosis
    (2014-01-01) Marcello Otake Sato; Megumi Sato; Kittipong Chaisiri; Wanna Maipanich; Tippayarat Yoonuan; Surapol Sanguankiat; Tiengkham Pongvongsa; Boungnong Boupha; Kazuhiko Moji; Jitra Waikagul; Universidade Federal do Tocantins; Niigata University; Mahidol University; Station of Malariology; Ministry of Health; National Institutes for the Humanities, Research Institute for Humanity and Nature
    Trichostrongylids infection has gained significant public health importance since Trichostrongylus spp. infections have been reported in humans in Lao PDR. In this study, gastrointestinal nematodes were identified and the intensity of infections was determined in goats and cattle, which are animals greatly used for meat production in Lahanam Village, Lao PDR. The total number of goats and bovines was 23 and 29, respectively, pertaining to 32 households surveyed in the area. Feacal samples were randomly collected from 14 goats and 11 bovines. Ninety three percent (13/14) of goats and 36% (3/11) of cattle were infected, with an average of 1,728 and 86 eggs per gram of faeces (EPG), respectively. Coproculture showed Trichostrongylus spp. (goats 16%; bovines 48%), Haemonchus spp. (goats 69%; bovines 37%), Cooperia spp. (bovines 8%) and Oesophagostomum spp. (goats 15%; bovines 6%). After performing the necropsy on an adult goat, Trichuris spp. was also found. We confirmed the presence of Oesophagostomum spp., H. contortus and T. colubriformis by morphology and DNA sequencing analysis of the ITS region of rDNA. Due to interactions between humans and goats in Lahanam Village and high EPG results, the diagnosis of species and the intensity of gastrointestinal nematode infection in these animals are important public-health issues. Other ruminant parasites, such as Oesophagostomum and Haemonchus, found in caprines and bovines, are reported to be causes of zoonosis and their presence in humans should be investigated in future field surveys in this area.
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    Nematode infection among ruminants in monsoon climate (Ban-Lahanam, Lao PDR) and its role as food-borne zoonosis
    (2014-03-01) Marcello O.take Otake; Megumi Sato; Kittipong Chaisiri; Wanna Maipanich; Tippayarat Yoonuan; Surapol Sanguankiat; Tiengkham Pongvongsa; Boungnong Boupha; Kazuhiko Moji; Jitra Waikagul; Universidade Federal do Tocantins; Niigata University School of Medicine; Mahidol University; Station of Malariology; Ministry of Health; National Institutes for the Humanities, Research Institute for Humanity and Nature
    Trichostrongylids infection has gained significant public health importance since Trichostrongylus spp. infections have been reported in humans in Lao PDR. In this study, gastrointestinal nematodes were identified and the intensity of infections was determined in goats and cattle, which are animals greatly used for meat production in Lahanam Village, Lao PDR. The total number of goats and bovines was 23 and 29, respectively, pertaining to 32 households surveyed in the area. Feacal samples were randomly collected from 14 goats and 11 bovines. Ninety three percent (13/14) of goats and 36% (3/11) of cattle were infected, with an average of 1,728 and 86 eggs per gram of faeces (EPG), respectively. Coproculture showed Trichostrongylus spp. (goats 16%; bovines 48%), Haemonchus spp. (goats 69%; bovines 37%), Cooperia spp. (bovines 8%) and Oesophagostomum spp. (goats 15%; bovines 6%). After performing the necropsy on an adult goat, Trichuris spp. was also found. We confirmed the presence of Oesophagostomum spp., H. contortus and T. colubriformis by morphology and DNA sequencing analysis of the ITS region of rDNA. Due to interactions between humans and goats in Lahanam Village and high EPG results, the diagnosis of species and the intensity of gastrointestinal nematode infection in these animals are important public-health issues. Other ruminant parasites, such as Oesophagostomum and Haemonchus, found in caprines and bovines, are reported to be causes of zoonosis and their presence in humans should be investigated in future field surveys in this area.
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    Patterns of trematode infections of Opisthorchis viverrini (Opisthorchiidae) and Haplorchis taichui (Heterophyidae) in human populations from two villages in Savannakhet Province, Lao PDR
    (2015-01-01) M. Sato; T. Pongvongsa; S. Sanguankiat; T. Yoonuan; J. Kobayashi; B. Boupha; F. Nishimoto; K. Moji; M. O. Sato; J. Waikagul; Niigata University School of Medicine; Station of Malariology; Mahidol University; University of the Ryukyus; Institute of Public Health; National Institutes for the Humanities, Research Institute for Humanity and Nature; Universidade Federal do Tocantins
    © Cambridge University Press 2014. The liver fluke, Opisthorchis viverrini, and the minute intestinal fluke, Haplorchis taichui, are prevalent in many Asian countries. This study analysed the patterns of infections of O. viverrini and H. taichui in Lahanam and Thakhamlien villages (Savannakhet Province, Lao PDR), in two cross-sectional investigations. Out of a total of 207 human participants, post-anthelmintic treatment positivity rates for expelled worms were 170 (82.1%) for H. taichui and 65 (31.4%) for O. viverrini. Both these species co-exist in the study villages. When each parasite was analysed separately, H. taichui infections reached a plateau among people aged >20 years. Opisthorchis viverrini infection rates were highest in the age group 21-30 years, with decreasing infection rates after the age of 30. Our findings indicated that fish-borne trematode infections were more prevalent among adults. Fish, common intermediate hosts, were acquired in the study area for analysis. The examination of 35 species of fish as intermediate hosts found O. viverrini metacercariae in only six species, and these were found mostly during the month of November. Many farmers who live on the rice fields obtain their food from their immediate environment, including these intermediate-host fish, potentially putting them at greater risk of O. viverrini infection. By contrast, H. taichui metacercariae were found in three species of fish obtained from the market, meaning that anyone could consume them and become infected. If people who work in rice fields limit the species of fish they consume, or avoid consuming raw fish during the month of November, they may reduce their risk of O. viverrini infection.
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    Short report: Human trichostrongylus colubriformis infection in a rural village in Laos
    (2011-01-01) Megumi Sato; Tippayarat Yoonuan; Surapol Sanguankiat; Supaporn Nuamtanong; Tiengkham Pongvongsa; Inthava Phimmayoi; Vilayphone Phanhanan; Boungnong Boupha; Kazuhiko Moji; Jitra Waikagul; Universidade Federal do Tocantins; Mahidol University; Station of Malariology; Ministry of Health; National Institutes for the Humanities, Research Institute for Humanity and Nature
    In Lahanam Village, Savannakhet Province, Laos, 125 of 253 villagers (49.4%) were found by fecal examination to harbor hookworm eggs. The eggs were heterogeneous in morphology and size, suggesting infections of mixed nematode species. To confirm the hookworm egg species, on a voluntary basis, 46 hookworm egg-positive participants were treated with albendazole, and post-treatment adult worms were collected from purged fecal samples. The common human hookworm was found in only 3 participants; 1 case of Necator americanus, and 2 cases of Ancylostoma duodenale. In contrast, adult Trichostrongylus worms were expelled from most participants (43 of 46, 93.5%). The Trichostrongylus species were confirmed by morphology and internal transcribed spacer 2 sequences; all worms were of the same species (T. colubriformis). In addition, some Trichostrongylus worms were obtained from a goat in the same village and identified as T. colubriformis. The results suggested that T. colubriformis was the main zoonotic species causing hookworm infections in the village. Copyright © 2011 by The American Society of Tropical Medicine and Hygiene.
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    Sympatric occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand
    (2007-01-01) Malinee T. Anantaphruti; Hiroshi Yamasaki; Minoru Nakao; Jitra Waikagul; Dorn Watthanakulpanich; Supaporn Nuamtanong; Wanna Maipanich; Somchit Pubampen; Surapol Sanguankiat; Chatree Muennoo; Kazuhiro Nakaya; Marcello O. Sato; Yasuhito Sako; Munehiro Okamoto; Akira Ito; Mahidol University; Asahikawa Medical University; Universidade Federal do Tocantins; Tottori University
    We confirmed sympatric occurrence of Taenia solium, T. saginata, and T. asiatica in western Thailand. DNA analysis of morphologically identified T. saginata, in a dual infection with T. solium, indicated it was T. asiatica. To our knowledge, this report is the first of T. asiatica and a dual Taenia infection from Thailand.
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    Systematics of the subfamily Haplorchiinae (Trematoda: Heterphyidae), based on nuclear ribosomal DNA genes and ITS2 region
    (2010-09-01) Urusa Thaenkham; Paron Dekumyoy; Chalit Komalamisra; Megumi Sato; Do Trung Dung; Jitra Waikagul; Mahidol University; Universidade Federal do Tocantins; National Institute of Malariology and Entomology
    Phylogenetic relationships of 6 species in the trematode subfamily Haplorchiinae were analyzed using small and large subunit of ribosomal DNA genes (18S rDNA and 28S rDNA) and internal transcribed spacer subunit II (ITS2) region as molecular markers. Maximum Likelihood and Bayesian inference analyses of combined rDNAs and ITS2 indicated a close relationship between the genera Haplorchis and Procerovum, while these two genera were distinct from Stellantchasmus falcatus. These phylogenetic relationships were consistent with the number of testes but not with the characters of the modification of the seminal vesicle or of the ventral sucker. Although three Haplorchis spp. were, together with Procerovum, in the same cluster, their mutual topology was incongruent between rDNA and ITS2 trees. Phylogenetic analyses using other molecular markers with more species are necessary to work out solid phylogenetic relationships among the species in this subfamily. © 2010 Elsevier Ireland Ltd.
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    Taenia
    (2015-01-01) Marcello Otake Sato; Caris Maroni Nunes; Megumi Sato; Jitra Waikagul; Universidade Federal do Tocantins; UNESP-Universidade Estadual Paulista; Niigata University School of Medicine; Mahidol University
    © 2015 by Taylor & Francis Group, LLC. Tapeworms are one of the most interesting causative agents of disease in humans and animals and have coexisted with humans since ancient times. Three Taenia species are currently accepted as human tapeworms (T. solium, T. saginata, and T. asiatica), and three Taenia species are classified as zoonotic species (T. multiceps, T. taeniaeformis, and T. crassiceps). In humans, two types of foodborne diseases are caused by Taenia: the adult worm infection called taeniasis and the infection caused by the metacestode forms. The infection caused by metacestodes of T. solium, T. saginata, T. asiatica, T. crassiceps, and T. taeniaeformis is called cysticercosis and if caused by T. multiceps is called coenurosis.