Browsing by Author "Yasuhito Sako"

Filter results by typing the first few letters
Now showing 1 - 6 of 6
  • No Thumbnail Available
    PublicationMetadata only
    Cysticercosis: IgG-ELISA evaluations of peak1 antigen and <30 kDa antigen of delipidized extract of Taenia solium metacestodes
    (2004-03-01) Paron Dekumyoy; Jitra Waikagul; Sirivan Vanijanonta; Malinee Thairungroj; Minoru Nakao; Yasuhito Sako; Sonoyo Watanabe; Akira Ito; Mahidol University; Asahikawa Medical University
    The antigenicity of ether-delipidized Taenia solium metacestode extract (DLPAg) was investigated by IgG-ELISA. The antigen showed higher antigenicity than that of non-delipidized antigen (NDLPAg). Then the DLPAg was subjected to Sephacryl S-200 gel chromatography and a partially purified antigen (DLPP1Ag) was identified as the promised antigen by IgG-ELISA using 25 sera from cysticercosis cases, 177 cases of 24 heterologous infections, and healthy controls. Sensitivity was 52% and specificity was 91.8% at the cut-off value (X̄+ 7SD), 0.399. Cross-reactivity occurred with 17 cases of eight diseases: cystic echinococcosis (7/11), taeniasis (1/16), gnathostomiasis (2/8), strongyloidiasis (1/12), angiostrongyliasis (1/12), paragonimiasis heterotremus (2/15), opisthorchiasis (1/9) and fascioliasis (2/7). When DLPP1Ag was fractionated through Ultra free centrifugal tube (retained 30 kDa) and Amicon (PM10), MWCOP1Ag (<30-10> kDa) was obtained; the antigen showed better results than DLPP1Ag with 88% sensitivity and 95.6% specificity at the cut-off value (X̄ + 4SD), 0.264. Nine cases of six diseases cross-reacted with this antigen: cystic echinococcosis (2/ 11), gnathostomiasis (2/8), trichinellosis (2/12), toxocariasis (1/5), schistosomiasis (1/6), and fascioliasis (1/7). MWCOP1Ag gave higher sensitivity than that of DLPP1Ag but some cross-reactivity occurred.
  • No Thumbnail Available
    PublicationMetadata only
    The first workshop towards the control of cestode zoonoses in Asia and Africa
    (2011-06-23) Akira Ito; Munehiro Okamoto; Tiaoying Li; Toni Wandra; Nyoman S. Dharmawan; Kadek I. Swastika; Paron Dekumyoy; Teera Kusolsuk; Abmed Davvajav; Anu Davaasuren; Temuulen Dorjsuren; Sissay M. Mekonnen; Zerihun H. Negasi; Tetsuya Yanagida; Yasuhito Sako; Minoru Nakao; Kazuhiro Nakaya; Antti J. Lavikainen; Agathe Nkouawa; Tahereh Mohammadzadeh; Asahikawa Medical University; Kyoto University; Sichuan Provincial CDC; Ministry of Health, Republic of Indonesia; Universitas Udayana; Mahidol University; Ministry of Health; Health Science University of Mongolia; Haramaya University; University of Helsinki Haartman Institute
    The first workshop towards the control of cestode zoonoses in Asia and Africa was held in Asahikawa Medical University, Japan on 15 and 16 Feb 2011. This meeting was fully supported by the Asian Science and Technology Strategic Cooperation Promotion Programs sponsored by the Special Coordination Funds for Promoting Science and Technology, the Ministry of Education Japan (MEXT) for 3 years from 2010 to Akira Ito. A total of 24 researchers from 9 countries joined together and discussed the present situation and problems towards the control of cestode zoonoses. As the meeting was simultaneously for the establishment of joint international, either bilateral or multilateral collaboration projects, the main purposes were directed to 1) how to detect taeniasis/cysticercosis infected patients, 2) how to differentiate Taenia solium from two other human Taenia species, T. saginata and T. asiatica, 3) how to evaluate T. asiatica based on the evidence of hybrid and hybrid-derived adult tapeworms from Thailand and China, 4) how to evaluate T. solium and T. hyaenae and other Taenia species from the wild animals in Ethiopia, and 5) how to detect echinococcosis patients and 6) how to differentiate Echinococcus species worldwide. Such important topics are summarized in this meeting report. © 2011 Ito et al; licensee BioMed Central Ltd.
  • No Thumbnail Available
    PublicationMetadata only
    Genotypic relationships between taenia saginata, taenia asiatica and their hybrids
    (2013-11-01) Kanako Yamane; Tetsuya Yanagida; Tiaoying Li; Xingwang Chen; Paron Dekumyoy; Jitra Waikagul; Agathe Nkouawa; Minoru Nakao; Yasuhito Sako; Akira Ito; Hiroshi Sato; Munehiro Okamoto; Yamaguchi University; Asahikawa Medical University; Centers for Disease Control and Prevention; Mahidol University; Kyoto University
    SUMMARY Partial sequences of the DNA polymerase delta (pold) gene from Taenia saginata-like adult worms were sequenced. Phylogenetic analysis revealed that pold gene sequences were clearly divided into two clades, differing from each other in five to seven nucleotides. There is little doubt that T. saginata and Taenia asiatica were once separated into two distinct taxa as has been concluded in previous studies. On the other hand, most of the adult worms, which were identified as T. asiatica using mitochondrial DNA, were homozygous for an allele that originated from the allele of T. saginata via single nucleotide substitution. These results indicate that most of the adult worms, which had been called T. asiatica, are not actually 'pure T. asiatica' but instead originated from the hybridization of 'pure T. saginata' and 'pure T. asiatica'. © 2013 Cambridge University Press.
  • No Thumbnail Available
    PublicationMetadata only
    Molecular and serological survey on taeniasis and cysticercosis in Kanchanaburi Province, Thailand
    (2010-09-01) Malinee T. Anantaphruti; Munehiro Okamoto; Tippayarat Yoonuan; Surapol Saguankiat; Teera Kusolsuk; Megumi Sato; Marcello O. Sato; Yasuhito Sako; Jitra Waikagul; Akira Ito; Mahidol University; Asahikawa Medical University; Tottori University
    A community-based field survey on taeniasis and cysticercosis was performed in two villages in Thong Pha Phum District, Kanchanaburi Province, central Thailand, where 3 Taenia species, T. solium, T. saginata and T. asiatica, are sympatrically occurring. Four (0.6%) out of 667 stool samples were egg-positive for Taenia sp. by Kato-Katz technique. Three out of those four persons and other three persons who were Taenia egg-negative but having a recent (<1 year) history of discharging worms in stool were treated with niclosamide. One Taenia egg-positive woman was not treated because of severe ascites. After treatment, three persons expelled long strobilae with scolices and two persons expelled strobilae without scolex. One Taenia egg-positive person did not expel any worms post-treatment. Among 5 persons, four expelled a single worm, whereas one expelled multiple worms, may be 6 worms but not confirmed by detection of scolices. One scolex was armed with hooklets, whereas 2 others did not. Multiplex PCR of 10 expelled proglottids (including 6 estimated worms from one patient) revealed that one sample was T. solium, one T. saginata, and 8 T. asiatica. A total of 159 residents agreed to receive a serological test for cysticercosis. By ELISA using partially purified glycoprotein antigen, 9 cases, 5 and 4 from villages A and B respectively, were found to be sero-positive. The five and an additional sample on the border line from village A were evaluated using confirmative immunoblot using recombinant chimeric antigen. Among the six samples, four including the border line sample were confirmed to be cysticercosis by immunoblotting. One of the 4 persons had neurological symptoms with nodular lesions in the brain by computed tomography. These 4 confirmed or suspected cysticercosis cases were free of T. solium worms, but two of them including confirmed NCC case had a past (>1 year) history of expelling proglottids in the stool. © 2010.
  • No Thumbnail Available
    PublicationMetadata only
    Sympatric occurrence of Taenia solium, T. saginata, and T. asiatica, Thailand
    (2007-01-01) Malinee T. Anantaphruti; Hiroshi Yamasaki; Minoru Nakao; Jitra Waikagul; Dorn Watthanakulpanich; Supaporn Nuamtanong; Wanna Maipanich; Somchit Pubampen; Surapol Sanguankiat; Chatree Muennoo; Kazuhiro Nakaya; Marcello O. Sato; Yasuhito Sako; Munehiro Okamoto; Akira Ito; Mahidol University; Asahikawa Medical University; Universidade Federal do Tocantins; Tottori University
    We confirmed sympatric occurrence of Taenia solium, T. saginata, and T. asiatica in western Thailand. DNA analysis of morphologically identified T. saginata, in a dual infection with T. solium, indicated it was T. asiatica. To our knowledge, this report is the first of T. asiatica and a dual Taenia infection from Thailand.
  • No Thumbnail Available
    PublicationMetadata only
    Taenia solium, Taenia saginata, Taenia asiatica, their hybrids and other helminthic infections occurring in a neglected tropical diseases' highly endemic area in Lao PDR
    (2018-02-08) Marcello Otake Sato; Megumi Sato; Tetsuya Yanagida; Jitra Waikagul; Tiengkham Pongvongsa; Yasuhito Sako; Surapol Sanguankiat; Tipparayat Yoonuan; Sengchanh Kounnavang; Satoru Kawai; Akira Ito; Munehiro Okamoto; Kazuhiko Moji; Niigata University; Yamaguchi University; Mahidol University; Nagasaki University; Kyoto University; Asahikawa Medical University; Dokkyo Medical University; Station of Malariology; Ministry of Health
    © 2018 Sato et al. Most part of Southeast Asia is considered endemic for human-infecting Taenia tapeworms; Taenia solium, T. saginata, and T. asiatica. However, until now there was no report of the occurrence of human cases of T. asiatica in Lao PDR. This study, conducted in Savannakhet Province, Lao PDR, microscopically examined a total of 470 fecal samples by Kato Katz method and found 86% of people harboring at least one helminth. Hookworms were detected in 56% of the samples besides Opisthorchis like eggs (42%), Trichuris trichiura (27%), Ascaris spp. (14%), and Taenia spp. (4%) eggs. Serology for cysticercosis showed 6.8% positives with results varying from 3% to 14.3% in Ethnic School students and Kalouk Kao village respectively. Species-specific PCR targeting mitochondrial DNA (mtDNA) of 28 tapeworms, recovered from 16 patients, revealed T. solium (n = 2), T. saginata (n = 21), and T. asiatica (n = 5). Two patients were confirmed to be coinfected with T. saginata and T. asiatica, indicating the endemicity of the 3 human Taenia in Lao PDR. However, nucleotide sequencing of a nuclear DNA gene, DNA polymerase delta (pold) revealed that all the tapeworms identified as T. asiatica using mtDNA had T. saginata type allele at pold locus, demonstrating that they are not “pure T. asiatica” but the hybrid descendants between the two species, confirming the wide distribution of hybrids of T. saginata/ T. asiatica in Southeast Asia. The high prevalence of several helminthic NTDs in east Savannakhet area even with conventional control measures indicates the importance to establish wide and multifaceted health programs to sustainably improve the quality of life of the populations living in these communities.