Publication: Redox mechanisms of AVS022, an oriental polyherbal formula, and its component herbs in protection against induction of matrix metalloproteinase-1 in UVA-irradiated keratinocyte HaCaT cells
Submitted Date
Received Date
Accepted Date
Issued Date
2013-10-21
Copyright Date
Announcement No.
Application No.
Patent No.
Valid Date
Resource Type
Edition
Resource Version
Language
File Type
No. of Pages/File Size
ISBN
ISSN
17414288
1741427X
1741427X
eISSN
Scopus ID
WOS ID
Pubmed ID
arXiv ID
Call No.
Other identifier(s)
2-s2.0-84885673285
Journal Title
Volume
Issue
item.page.oaire.edition
Start Page
End Page
Access Rights
Access Status
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Physical Location
Bibliographic Citation
Evidence-based Complementary and Alternative Medicine. Vol.2013, (2013)
Citation
Thanyawan Pluemsamran, Pinpat Tripatara, Rattana Phadungrakwittaya, Pravit Akarasereenont, Tawee Laohapand, Uraiwan Panich (2013). Redox mechanisms of AVS022, an oriental polyherbal formula, and its component herbs in protection against induction of matrix metalloproteinase-1 in UVA-irradiated keratinocyte HaCaT cells. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/32108.
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Redox mechanisms of AVS022, an oriental polyherbal formula, and its component herbs in protection against induction of matrix metalloproteinase-1 in UVA-irradiated keratinocyte HaCaT cells
Alternative Title(s)
Author's Affiliation
Author's E-mail
Editor(s)
Editor's Affiliation
Corresponding Author(s)
Creator(s)
Compiler
Advisor(s)
Illustrator(s)
Applicant(s)
Inventor(s)
Issuer
Assignee
Other Contributor(s)
Series
Has Part
Abstract
Ayurved Siriraj HaRak (AVS022) formula has been used for topical remedy of dermatologic disorders. Oxidative stress induced by ultraviolet (UV) A irradiation could be implicated in photoaged skin through triggering matrix metalloproteinase-1 (MMP-1). We, therefore, explored the antioxidant mechanisms by which AVS022 formulation and its individual components protected against UVA-dependent MMP-1 upregulation in keratinocyte HaCaT cells. TLC analysis revealed the presence of multiple phenolics including gallic acid (GA) in the AVS022 extracts. We demonstrated that pretreatment with the whole formula and individual herbal components except T. triandra protected against increased MMP-1 activity in irradiated HaCaT cells. Moreover, all herbal extracts and GA, used as the reference compound, were able to reverse cytotoxicity, oxidant production, glutathione (GSH) loss, and inactivation of catalase and glutathione peroxidase (GPx). F. racemosa was observed to yield the strongest abilities to abolish UVA-mediated induction of MMP-1 and impairment of antioxidant defenses including GSH and catalase. Our observations suggest that upregulation of endogenous antioxidants could be the mechanisms by which AVS022 and its herbal components suppressed UVA-stimulated MMP-1 in HaCaT cells. In addition, pharmacological actions of AVS022 formula may be attributed to the antioxidant potential of its components, in particular F. racemosa, and several phenolics including GA. © 2013 Thanyawan Pluemsamran et al.