Comparison of two algorithms of APTT-based lupus anticoagulant assay, two Dilute Russell viper venom time reagents, and silica clotting time
Issued Date
2026-01-01
Resource Type
eISSN
19326203
Scopus ID
2-s2.0-105043874292
Pubmed ID
42361075
Journal Title
Plos One
Volume
21
Issue
6
Rights Holder(s)
SCOPUS
Bibliographic Citation
Plos One Vol.21 No.6 (2026) , e0352430
Suggested Citation
Wongkrajang P., Pientong T., Hanyongyuth R., Tientadakul P. Comparison of two algorithms of APTT-based lupus anticoagulant assay, two Dilute Russell viper venom time reagents, and silica clotting time. Plos One Vol.21 No.6 (2026) , e0352430. doi:10.1371/journal.pone.0352430 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/117957
Title
Comparison of two algorithms of APTT-based lupus anticoagulant assay, two Dilute Russell viper venom time reagents, and silica clotting time
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Abstract
Lupus anticoagulants (LA) are heterogeneous antiphospholipid antibodies that interfere with phospholipid-dependent coagulation assays, resulting in considerable variability among detection methods. Although international guidelines recommend a stepwise approach incorporating screening, mixing, and confirmatory testing, integrated strategies omitting routine mixing studies are widely used. Different percentile-based cutoffs have been proposed for defining LA positivity.We retrospectively analyzed 135 citrated plasma samples requested for LA testing. Five LA detection approaches were evaluated: 4 integrated assays and 1 activated partial thromboplastin time (APTT)-based approach following the ISTH-recommended stepwise algorithm with a mixing study. The integrated assays comprised silica clotting time, dilute Russell viper venom time (dRVVT) using 2 different reagent systems, and an APTT-based assay. Precision studies and reference intervals were established, and LA positivity rates were compared using 97.5th and 99th percentile cutoffs. Inter-assay agreement and associations with anticardiolipin (aCL) and anti-β2-glycoprotein I (aβ2GPI) antiphospholipid antibodies were assessed. LA positivity rates varied across procedures (19.3%-35.6%) at the 97.5th percentile. Application of the 99th percentile decreased positivity for dRVVT-HemosIL and APTT-based assays. Positivity increased at the 97.5th percentile in patients tested according to ISTH indications, with minimal impact in noncompliant cases. Inter-assay agreement ranged from fair to substantial and was influenced by assay type and cutoff definition. APTT-based assays showed the strongest associations with aCL and aβ2GPI antibodies. LA detection is strongly influenced by assay selection and cutoff strategy. Positivity rate-based evaluation provides a practical framework for comparing LA assays in laboratory practice, particularly in the absence of a reference standard.