Ryosuke SuzukiTomohiro IshikawaEiji KonishiMami MatsudaKoichi WatashiHideki AizakiTomohiko TakasakiTakaji WakitaNational Institute of Infectious DiseasesDokkyo Medical UniversityMahidol University2018-11-092018-11-092014-01-01Journal of General Virology. Vol.95, No.PART 1 (2014), 60-6514652099002213172-s2.0-84890277182https://repository.li.mahidol.ac.th/handle/20.500.14594/34022A method for rapid production of single-round infectious particles (SRIPs) of flavivirus would be useful for viral mutagenesis studies. Here, we established a DNA-based production system for SRIPs of flavivirus. We constructed a Japanese encephalitis virus (JEV) subgenomic replicon plasmid, which lacked the C-prM-E (capsid-pre-membrane-envelope) coding region, under the control of the cytomegalovirus promoter. When the JEV replicon plasmid was transiently co-transfected with a JEV C-prM-E expression plasmid into 293T cells, SRIPs were produced, indicating successful trans-complementation with JEV structural proteins. Equivalent production levels were observed when C and prM-E proteins were provided separately. Furthermore, dengue types 1-4, West Nile, yellow fever or tick-borne encephalitis virus prM-E proteins could be utilized for production of chimaeric flavivirus SRIPs, although the production was less efficient for dengue and yellow fever viruses. These results indicated that our plasmid-based system is suitable for investigating the life cycles of flaviviruses, diagnostic applications and development of safer vaccine candidates. © 2014 SGM.Mahidol UniversityImmunology and MicrobiologyArticleSCOPUS10.1099/vir.0.058008-0