Chia seed mucilage biosorbent synergism with hydrophobic iron (III)-natural phenolic nanoparticles for dispersive solid-phase extraction of tetracycline residues in milk and honey samples before HPLC analysis

Nakhonchai N.Ponhong K.Sweedler J.V.Lee C.Y.Supharoek S.a.Mahidol University2025-09-132025-09-132025-12-01Food Chemistry Vol.494 (2025)03088146https://repository.li.mahidol.ac.th/handle/123456789/112024This study utilized the synergistic effectiveness of chia seed mucilage and iron (III)-natural phenolic nanoparticles as biosorbents for the first time in the dispersive solid-phase extraction (DSPE) of oxytetracycline, tetracycline, chlortetracycline, and doxycycline followed by HPLC-UV quantification. An in-situ iron (III)-natural phenolic solid adsorbent was created using natural phenolics found in the copper pod tree bark. An ultrasonic-assisted extraction was performed to enhance the extraction efficiency of DSPE-based biosorbents. Excellent linearity with R2 greater than 0.9957 was achieved, with detection limits between 0.7 and 2.0 μg L−1 for milk, 2.5 and 7.5 μg kg−1 for honey, and precisions below 9.1 %. The DSPE-based biosorbent was effectively applied to quantify tetracycline residues in milk and honey samples, yielding recoveries from 72.1 to 116.2 %. This method showed potential as an alternative simple, low-cost, eco-friendly, biodegradable, and reusable biosorbent to determine tetracycline residues in food.ChemistryAgricultural and Biological SciencesChia seed mucilage biosorbent synergism with hydrophobic iron (III)-natural phenolic nanoparticles for dispersive solid-phase extraction of tetracycline residues in milk and honey samples before HPLC analysisArticleSCOPUS10.1016/j.foodchem.2025.1462392-s2.0-10501514460718737072